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• W2114070149 endingPage "2309" @default.
• W2114070149 startingPage "2298" @default.
• W2114070149 abstract "ABSTRACT In this study, infection of 293/ACE2 cells with severe acute respiratory syndrome coronavirus (SARS-CoV) activated several apoptosis-associated events, namely, cleavage of caspase-3, caspase-8, and poly(ADP-ribose) polymerase 1 (PARP), and chromatin condensation and the phosphorylation and hence inactivation of the eukaryotic translation initiation factor 2α (eIF2α). In addition, two of the three cellular eIF2α kinases known to be virus induced, protein kinase R (PKR) and PKR-like endoplasmic reticulum kinase (PERK), were activated by SARS-CoV. The third kinase, general control nonderepressible-2 kinase (GCN2), was not activated, but late in infection the level of GCN2 protein was significantly reduced. Reverse transcription-PCR analyses revealed that the reduction of GCN2 protein was not due to decreased transcription or stability of GCN2 mRNA. The specific reduction of PKR protein expression by antisense peptide-conjugated phosphorodiamidate morpholino oligomers strongly reduced cleavage of PARP in infected cells. Surprisingly, the knockdown of PKR neither enhanced SARS-CoV replication nor abrogated SARS-CoV-induced eIF2α phosphorylation. Pretreatment of cells with beta interferon prior to SARS-CoV infection led to a significant decrease in PERK activation, eIF2α phosphorylation, and SARS-CoV replication. The various effects of beta interferon treatment were found to function independently on the expression of PKR. Our results show that SARS-CoV infection activates PKR and PERK, leading to sustained eIF2α phosphorylation. However, virus replication was not impaired by these events, suggesting that SARS-CoV possesses a mechanism to overcome the inhibitory effects of phosphorylated eIF2α on viral mRNA translation. Furthermore, our data suggest that viral activation of PKR can lead to apoptosis via a pathway that is independent of eIF2α phosphorylation." @default.
• W2114070149 created "2016-06-24" @default.
• W2114070149 creator A5009334726 @default.
• W2114070149 creator A5030646943 @default.
• W2114070149 creator A5049658579 @default.
• W2114070149 creator A5073408781 @default.
• W2114070149 creator A5088808959 @default.
• W2114070149 date "2009-03-01" @default.
• W2114070149 modified "2023-10-14" @default.
• W2114070149 title "Severe Acute Respiratory Syndrome Coronavirus Triggers Apoptosis via Protein Kinase R but Is Resistant to Its Antiviral Activity" @default.
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• W2114070149 doi "https://doi.org/10.1128/jvi.01245-08" @default.
• W2114070149 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2643707" @default.
• W2114070149 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19109397" @default.
• W2114070149 hasPublicationYear "2009" @default.
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