FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2114077995> ?p ?o ?g. }

• W2114077995 endingPage "280" @default.
• W2114077995 startingPage "271" @default.
• W2114077995 abstract "Hypertrophic scarring remains the most disabling sequela for burn survivors. Little is known about its pathogenesis. Collagen accumulation, however, has been consistently observed in burn hypertrophic scars (HS).We have studied collagen production in the dermal fibroblasts derived from HS, which has developed for 9 months to 2 years. Reconstructive surgery was performed to remove HS from which the fibroblasts were cultured. Similarly, the normal cells were grown from the patient's donor site (DS), which provided autografting to the HS site. Collagen production in HS and DS fibroblasts was compared and analyzed in minimal essential amino acid medium containing 5% fetal bovine serum with inclusion of L-ascorbic acid (100 microg/mL) and beta-aminopropoinitrile (100 microg/mL) by monitoring a 20-h [3H]proline incorporation into bacterial collagenase III-digestible protein in the conditioned media.We failed to detect any significant difference in collagen production in vitro between HS and DS. Irrespective of the fibroblasts from HS or DS, collagen production was substantially stimulated by human recombinant transforming growth factor beta-1 (TGF-beta1) (20 ng/mL) by approximately 250% after a 3-day pretreatment. In contrast, sodium nitroprusside (SNP) at 100 microM exhibited significant suppression (68%), which was rescued by hemoglobin (10 microM). TGF-beta1 significantly decreased nitric oxide (NO) production by 55%. In contrast, NO level drastically increased by 350% following SNP treatment. Epidermal growth factor showed no effect on either collagen production or NO level. The linear regression analysis shows a significant inverse correlation (r = 0.72; p < 0.05) of NO level with collagen production, suggesting the involvement of NO signaling in the modulation of collagen production. Consistent with the notion, we further showed that N-nitro-L-arginine methyl ester (100 microM) caused a synergistic stimulation and an arrested inhibition of collagen production in the presence of TGFbeta-1 and SNP, respectively. 8-BrcGMP (300 microM) mimicked the NO inhibitory action, while methylene blue (50 microM) restored the collagen production which was inhibited by SNP. Moreover, 8-BrcGMP offset the stimulation of collagen production.The dermal fibroblasts derived from HS were not different from normals with respect to collagen production and their responses to regulations. The inhibition of collagen production was achieved by a cGMP-dependent NO action. TGFbeta-1 inhibited NO/cGMP signaling to ensure its stimulatory effect on collagen production in the dermal fibroblasts." @default.
• W2114077995 created "2016-06-24" @default.
• W2114077995 creator A5049826824 @default.
• W2114077995 creator A5075128319 @default.
• W2114077995 date "1999-03-01" @default.
• W2114077995 modified "2023-10-02" @default.
• W2114077995 title "Up-regulation by human recombinant transforming growth factor β-1 of collagen production in cultured dermal fibroblasts is mediated by the inhibition of nitric oxide signaling" @default.
• W2114077995 cites W1547758697 @default.
• W2114077995 cites W1718691538 @default.
• W2114077995 cites W1775749144 @default.
• W2114077995 cites W1834041853 @default.
• W2114077995 cites W1883906051 @default.
• W2114077995 cites W1964195356 @default.
• W2114077995 cites W1964501206 @default.
• W2114077995 cites W1973535328 @default.
• W2114077995 cites W1980937371 @default.
• W2114077995 cites W1981595227 @default.
• W2114077995 cites W1990802251 @default.
• W2114077995 cites W2007237158 @default.
• W2114077995 cites W2041994889 @default.
• W2114077995 cites W2070005444 @default.
• W2114077995 cites W2073973931 @default.
• W2114077995 cites W2081480546 @default.
• W2114077995 cites W2084162464 @default.
• W2114077995 cites W2084419824 @default.
• W2114077995 cites W2085804760 @default.
• W2114077995 cites W2107732949 @default.
• W2114077995 cites W2114633584 @default.
• W2114077995 cites W2114696344 @default.
• W2114077995 cites W2124559417 @default.
• W2114077995 cites W2155615934 @default.
• W2114077995 cites W2169858199 @default.
• W2114077995 cites W2188060873 @default.
• W2114077995 cites W2215360874 @default.
• W2114077995 cites W2419357110 @default.
• W2114077995 cites W39614084 @default.
• W2114077995 cites W4211052802 @default.
• W2114077995 doi "https://doi.org/10.1016/s1072-7515(98)00303-2" @default.
• W2114077995 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10065816" @default.
• W2114077995 hasPublicationYear "1999" @default.
• W2114077995 type Work @default.
• W2114077995 sameAs 2114077995 @default.
• W2114077995 citedByCount "43" @default.
• W2114077995 countsByYear W21140779952012 @default.
• W2114077995 countsByYear W21140779952013 @default.
• W2114077995 countsByYear W21140779952014 @default.
• W2114077995 countsByYear W21140779952015 @default.
• W2114077995 countsByYear W21140779952016 @default.
• W2114077995 countsByYear W21140779952017 @default.
• W2114077995 countsByYear W21140779952018 @default.
• W2114077995 countsByYear W21140779952020 @default.
• W2114077995 countsByYear W21140779952021 @default.
• W2114077995 countsByYear W21140779952022 @default.
• W2114077995 crossrefType "journal-article" @default.
• W2114077995 hasAuthorship W2114077995A5049826824 @default.
• W2114077995 hasAuthorship W2114077995A5075128319 @default.
• W2114077995 hasConcept C118131993 @default.
• W2114077995 hasConcept C126322002 @default.
• W2114077995 hasConcept C134018914 @default.
• W2114077995 hasConcept C142724271 @default.
• W2114077995 hasConcept C16685009 @default.
• W2114077995 hasConcept C170493617 @default.
• W2114077995 hasConcept C202751555 @default.
• W2114077995 hasConcept C2775960820 @default.
• W2114077995 hasConcept C2777624698 @default.
• W2114077995 hasConcept C2778698245 @default.
• W2114077995 hasConcept C2779404806 @default.
• W2114077995 hasConcept C2779610285 @default.
• W2114077995 hasConcept C2780381497 @default.
• W2114077995 hasConcept C2780942790 @default.
• W2114077995 hasConcept C2986274086 @default.
• W2114077995 hasConcept C31903555 @default.
• W2114077995 hasConcept C519581460 @default.
• W2114077995 hasConcept C55493867 @default.
• W2114077995 hasConcept C64348721 @default.
• W2114077995 hasConcept C71924100 @default.
• W2114077995 hasConcept C86803240 @default.
• W2114077995 hasConceptScore W2114077995C118131993 @default.
• W2114077995 hasConceptScore W2114077995C126322002 @default.
• W2114077995 hasConceptScore W2114077995C134018914 @default.
• W2114077995 hasConceptScore W2114077995C142724271 @default.
• W2114077995 hasConceptScore W2114077995C16685009 @default.
• W2114077995 hasConceptScore W2114077995C170493617 @default.
• W2114077995 hasConceptScore W2114077995C202751555 @default.
• W2114077995 hasConceptScore W2114077995C2775960820 @default.
• W2114077995 hasConceptScore W2114077995C2777624698 @default.
• W2114077995 hasConceptScore W2114077995C2778698245 @default.
• W2114077995 hasConceptScore W2114077995C2779404806 @default.
• W2114077995 hasConceptScore W2114077995C2779610285 @default.
• W2114077995 hasConceptScore W2114077995C2780381497 @default.
• W2114077995 hasConceptScore W2114077995C2780942790 @default.
• W2114077995 hasConceptScore W2114077995C2986274086 @default.
• W2114077995 hasConceptScore W2114077995C31903555 @default.
• W2114077995 hasConceptScore W2114077995C519581460 @default.
• W2114077995 hasConceptScore W2114077995C55493867 @default.
• W2114077995 hasConceptScore W2114077995C64348721 @default.
• W2114077995 hasConceptScore W2114077995C71924100 @default.
• W2114077995 hasConceptScore W2114077995C86803240 @default.

• next