FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2114288032> ?p ?o ?g. }

• W2114288032 endingPage "3368" @default.
• W2114288032 startingPage "3359" @default.
• W2114288032 abstract "ABSTRACT Much of the gene content of the human gastric pathogen Helicobacter pylori (∼1.7-Mb genome) is considered essential. This view is based on the completeness of metabolic pathways, infrequency of nutritional auxotrophies, and paucity of pathway redundancies typically found in bacteria with larger genomes. Thus, genetic analysis of gene function is often hampered by lethality. In the absence of controllable promoters, often used to titrate gene function, we investigated the feasibility of an antisense RNA interference strategy. To test the antisense approach, we targeted alkyl hydroperoxide reductase (AhpC), one of the most abundant proteins expressed by H. pylori and one whose function is essential for both in vitro growth and gastric colonization. Here, we show that antisense ahpC (as- ahpC ) RNA expression from shuttle vector pDH37::as- ahpC achieved an ∼72% knockdown of AhpC protein levels, which correlated with increased susceptibilities to hydrogen peroxide, cumene, and tert -butyl hydroperoxides but not with growth efficiency. Compensatory increases in catalase levels were not observed in the knockdowns. Expression of single-copy antisense constructs (expressed under the urease promoter and containing an fd phage terminator) from the rdxA locus of mouse-colonizing strain X47 achieved a 32% knockdown of AhpC protein levels (relative to wild-type X47 levels), which correlated with increased susceptibility to organic peroxides but not with mouse colonization efficiency. Our studies indicate that high levels of AhpC are not required for in vitro growth or for primary gastric colonization. Perhaps AhpC, like catalase, assumes a greater role in combating exogenous peroxides arising from lifelong chronic inflammation. These studies also demonstrate the utility of antisense RNA interference in the evaluation of gene function in H. pylori ." @default.
• W2114288032 created "2016-06-24" @default.
• W2114288032 creator A5005001853 @default.
• W2114288032 creator A5013551932 @default.
• W2114288032 creator A5030923798 @default.
• W2114288032 date "2007-05-01" @default.
• W2114288032 modified "2023-09-30" @default.
• W2114288032 title "Antisense RNA Modulation of Alkyl Hydroperoxide Reductase Levels in <i>Helicobacter pylori</i> Correlates with Organic Peroxide Toxicity but Not Infectivity" @default.
• W2114288032 cites W1488299617 @default.
• W2114288032 cites W1584239323 @default.
• W2114288032 cites W1668019941 @default.
• W2114288032 cites W1768247331 @default.
• W2114288032 cites W1940842012 @default.
• W2114288032 cites W1980958737 @default.
• W2114288032 cites W1984499682 @default.
• W2114288032 cites W1995078929 @default.
• W2114288032 cites W1999650747 @default.
• W2114288032 cites W2003760248 @default.
• W2114288032 cites W2014560396 @default.
• W2114288032 cites W2020834967 @default.
• W2114288032 cites W2022472102 @default.
• W2114288032 cites W2027983757 @default.
• W2114288032 cites W2036408983 @default.
• W2114288032 cites W2039095577 @default.
• W2114288032 cites W2039654209 @default.
• W2114288032 cites W2048220832 @default.
• W2114288032 cites W2064156350 @default.
• W2114288032 cites W2080553284 @default.
• W2114288032 cites W2088570910 @default.
• W2114288032 cites W2091296748 @default.
• W2114288032 cites W2098795538 @default.
• W2114288032 cites W2099596694 @default.
• W2114288032 cites W2100422393 @default.
• W2114288032 cites W2101166931 @default.
• W2114288032 cites W2101736537 @default.
• W2114288032 cites W2102713587 @default.
• W2114288032 cites W2105277740 @default.
• W2114288032 cites W2108380588 @default.
• W2114288032 cites W2110132014 @default.
• W2114288032 cites W2111909489 @default.
• W2114288032 cites W2112758830 @default.
• W2114288032 cites W2115567113 @default.
• W2114288032 cites W2116056203 @default.
• W2114288032 cites W2121455793 @default.
• W2114288032 cites W2125311076 @default.
• W2114288032 cites W2126888285 @default.
• W2114288032 cites W2128079705 @default.
• W2114288032 cites W2130168792 @default.
• W2114288032 cites W2134314271 @default.
• W2114288032 cites W2134581757 @default.
• W2114288032 cites W2139350259 @default.
• W2114288032 cites W2140431060 @default.
• W2114288032 cites W2142136966 @default.
• W2114288032 cites W2145928913 @default.
• W2114288032 cites W2149770337 @default.
• W2114288032 cites W2150834599 @default.
• W2114288032 cites W2154901892 @default.
• W2114288032 cites W2155544436 @default.
• W2114288032 cites W2158657807 @default.
• W2114288032 cites W2158815960 @default.
• W2114288032 cites W2159595936 @default.
• W2114288032 cites W2169674958 @default.
• W2114288032 cites W2169758355 @default.
• W2114288032 cites W2323815710 @default.
• W2114288032 doi "https://doi.org/10.1128/jb.00012-07" @default.
• W2114288032 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1855876" @default.
• W2114288032 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17337572" @default.
• W2114288032 hasPublicationYear "2007" @default.
• W2114288032 type Work @default.
• W2114288032 sameAs 2114288032 @default.
• W2114288032 citedByCount "27" @default.
• W2114288032 countsByYear W21142880322012 @default.
• W2114288032 countsByYear W21142880322013 @default.
• W2114288032 countsByYear W21142880322014 @default.
• W2114288032 countsByYear W21142880322015 @default.
• W2114288032 countsByYear W21142880322016 @default.
• W2114288032 countsByYear W21142880322021 @default.
• W2114288032 countsByYear W21142880322022 @default.
• W2114288032 crossrefType "journal-article" @default.
• W2114288032 hasAuthorship W2114288032A5005001853 @default.
• W2114288032 hasAuthorship W2114288032A5013551932 @default.
• W2114288032 hasAuthorship W2114288032A5030923798 @default.
• W2114288032 hasBestOaLocation W21142880322 @default.
• W2114288032 hasConcept C104317684 @default.
• W2114288032 hasConcept C153911025 @default.
• W2114288032 hasConcept C166703698 @default.
• W2114288032 hasConcept C173396325 @default.
• W2114288032 hasConcept C181199279 @default.
• W2114288032 hasConcept C202416437 @default.
• W2114288032 hasConcept C203233990 @default.
• W2114288032 hasConcept C2778979269 @default.
• W2114288032 hasConcept C55493867 @default.
• W2114288032 hasConcept C67705224 @default.
• W2114288032 hasConcept C86803240 @default.
• W2114288032 hasConcept C89423630 @default.
• W2114288032 hasConceptScore W2114288032C104317684 @default.
• W2114288032 hasConceptScore W2114288032C153911025 @default.
• W2114288032 hasConceptScore W2114288032C166703698 @default.

• next