FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2114297659> ?p ?o ?g. }

• W2114297659 endingPage "1488" @default.
• W2114297659 startingPage "1479" @default.
• W2114297659 abstract "Abstract The culture levels of glucose and CO 2 have been reported to independently have important influences on mammalian cell processes. In this work the combined effects of glucose limitation and CO 2 partial pressure ( p CO 2 ) on monoclonal antibody (IgG) producing Chinese Hamster Ovary cells were investigated in a perfusion reactor operated with controlled cell specific medium feed rate, pH and osmolality. Under high glucose conditions (14.3 ± 0.8 mM), the apparent growth rate decreased (from 0.021 to 0.009 h −1 ) as the p CO 2 increased to ∼220 mmHg, while the cell specific IgG productivity was almost unchanged. The lactate yield from glucose was not affected by p CO 2 up to ∼220 mmHg and glucose was mainly converted to lactate. A feed medium modification from high (33 mM) to low (6 mM) glucose resulted in <0.1 mM glucose in the culture. As a result of apparently shifting metabolism towards the conversion of pyruvate to CO 2 , both the ratio of lactate to glucose and the alanine production rate were lowered (1.51–1.14 and 17.7–0.56 nmol/10 6 cells h, respectively). Interestingly, when the p CO 2 was increased to ∼140 mmHg, limiting glucose resulted in 1.7‐fold higher growth rates, compared to high glucose conditions. However, at ∼220 mmHg p CO 2 this beneficial effect of glucose limitation on these CHO cells was lost as the growth rate dropped dramatically to 0.008 h −1 and the IgG productivity was lowered by 15% ( P < 0.01) relative to the high glucose condition. The IgG galactosylation increased under glucose‐ limited compared to high‐glucose conditions Biotechnol. Bioeng. 2007;97:1479–1488. © 2007 Wiley Periodicals, Inc." @default.
• W2114297659 created "2016-06-24" @default.
• W2114297659 creator A5021231351 @default.
• W2114297659 creator A5067339209 @default.
• W2114297659 creator A5085736100 @default.
• W2114297659 date "2007-02-22" @default.
• W2114297659 modified "2023-10-15" @default.
• W2114297659 title "Dependence on glucose limitation of thepCO2 influences on CHO cell growth, metabolism and IgG production" @default.
• W2114297659 cites W1270599943 @default.
• W2114297659 cites W1513373195 @default.
• W2114297659 cites W1974010414 @default.
• W2114297659 cites W1980767285 @default.
• W2114297659 cites W1995067802 @default.
• W2114297659 cites W2002029926 @default.
• W2114297659 cites W2002172128 @default.
• W2114297659 cites W2004715096 @default.
• W2114297659 cites W2020840252 @default.
• W2114297659 cites W2023178000 @default.
• W2114297659 cites W2025138920 @default.
• W2114297659 cites W2028813771 @default.
• W2114297659 cites W2060901195 @default.
• W2114297659 cites W2061226704 @default.
• W2114297659 cites W2066174543 @default.
• W2114297659 cites W2067282541 @default.
• W2114297659 cites W2067747765 @default.
• W2114297659 cites W2083198296 @default.
• W2114297659 cites W2083441961 @default.
• W2114297659 cites W2087022377 @default.
• W2114297659 cites W2087058811 @default.
• W2114297659 cites W2089696140 @default.
• W2114297659 cites W2107597623 @default.
• W2114297659 cites W2109355820 @default.
• W2114297659 cites W2117057407 @default.
• W2114297659 cites W2119641678 @default.
• W2114297659 cites W2121811155 @default.
• W2114297659 cites W2122236129 @default.
• W2114297659 cites W2130250535 @default.
• W2114297659 cites W2196751920 @default.
• W2114297659 doi "https://doi.org/10.1002/bit.21376" @default.
• W2114297659 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17318909" @default.
• W2114297659 hasPublicationYear "2007" @default.
• W2114297659 type Work @default.
• W2114297659 sameAs 2114297659 @default.
• W2114297659 citedByCount "32" @default.
• W2114297659 countsByYear W21142976592012 @default.
• W2114297659 countsByYear W21142976592013 @default.
• W2114297659 countsByYear W21142976592014 @default.
• W2114297659 countsByYear W21142976592015 @default.
• W2114297659 countsByYear W21142976592016 @default.
• W2114297659 countsByYear W21142976592017 @default.
• W2114297659 countsByYear W21142976592018 @default.
• W2114297659 countsByYear W21142976592019 @default.
• W2114297659 countsByYear W21142976592020 @default.
• W2114297659 countsByYear W21142976592021 @default.
• W2114297659 crossrefType "journal-article" @default.
• W2114297659 hasAuthorship W2114297659A5021231351 @default.
• W2114297659 hasAuthorship W2114297659A5067339209 @default.
• W2114297659 hasAuthorship W2114297659A5085736100 @default.
• W2114297659 hasBestOaLocation W21142976591 @default.
• W2114297659 hasConcept C126322002 @default.
• W2114297659 hasConcept C134018914 @default.
• W2114297659 hasConcept C165220095 @default.
• W2114297659 hasConcept C170493617 @default.
• W2114297659 hasConcept C17093226 @default.
• W2114297659 hasConcept C175656101 @default.
• W2114297659 hasConcept C185592680 @default.
• W2114297659 hasConcept C2779306644 @default.
• W2114297659 hasConcept C2780963292 @default.
• W2114297659 hasConcept C54355233 @default.
• W2114297659 hasConcept C55493867 @default.
• W2114297659 hasConcept C62231903 @default.
• W2114297659 hasConcept C71924100 @default.
• W2114297659 hasConcept C81885089 @default.
• W2114297659 hasConcept C86803240 @default.
• W2114297659 hasConceptScore W2114297659C126322002 @default.
• W2114297659 hasConceptScore W2114297659C134018914 @default.
• W2114297659 hasConceptScore W2114297659C165220095 @default.
• W2114297659 hasConceptScore W2114297659C170493617 @default.
• W2114297659 hasConceptScore W2114297659C17093226 @default.
• W2114297659 hasConceptScore W2114297659C175656101 @default.
• W2114297659 hasConceptScore W2114297659C185592680 @default.
• W2114297659 hasConceptScore W2114297659C2779306644 @default.
• W2114297659 hasConceptScore W2114297659C2780963292 @default.
• W2114297659 hasConceptScore W2114297659C54355233 @default.
• W2114297659 hasConceptScore W2114297659C55493867 @default.
• W2114297659 hasConceptScore W2114297659C62231903 @default.
• W2114297659 hasConceptScore W2114297659C71924100 @default.
• W2114297659 hasConceptScore W2114297659C81885089 @default.
• W2114297659 hasConceptScore W2114297659C86803240 @default.
• W2114297659 hasIssue "6" @default.
• W2114297659 hasLocation W21142976591 @default.
• W2114297659 hasLocation W21142976592 @default.
• W2114297659 hasOpenAccess W2114297659 @default.
• W2114297659 hasPrimaryLocation W21142976591 @default.
• W2114297659 hasRelatedWork W1581020121 @default.
• W2114297659 hasRelatedWork W1980130043 @default.
• W2114297659 hasRelatedWork W2019371398 @default.
• W2114297659 hasRelatedWork W2036340686 @default.

• next