SemOpenAlex |

SemOpenAlex

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2114393976> ?p ?o ?g. }

Showing items 1 to 100 of ±136 with 100 items per page.

W2114393976 endingPage "434" @default.
W2114393976 startingPage "423" @default.
W2114393976 abstract "Hedgehog (HH) signaling has recently been associated with cartilage degradation in osteoarthritis (OA). Because interleukin-1β (IL-1β) has been implicated as a principal instigator of OA, we sought to determine whether IL-1β induces the expression of sonic HH (SHH) and its regulation by microRNAs (miRNAs) in human chondrocytes.Expression of SHH protein in human OA cartilage and in an animal model of OA was determined by immunohistochemical analysis and immunofluorescence analysis, respectively. Gene and protein expression in IL-1β- or SHH-stimulated OA chondrocytes was determined by TaqMan assays and Western blotting, respectively. The effect of overexpression of miRNA-602 (miR-602) and miR-608 or their antagomirs on SHH expression was evaluated by transient transfection of human chondrocytes and HEK 293 cells. The role of signaling pathways was evaluated using small molecule inhibitors. Binding of miRNAs with the putative seed sequence in SHH messenger RNA (mRNA) was validated using a luciferase reporter assay.Expression of SHH, patched 1, Gli-1, HH-interacting protein, matrix metalloproteinase 13 (MMP-13), and Colα1(X) was high in damaged OA cartilage. In damaged cartilage and in IL-1β-stimulated OA chondrocytes, expression of SHH was inversely correlated with expression of miR-608. Cotransfection of OA chondrocytes with miR-608 or miR-602 mimic inhibited reporter activity, and mutation of the miRNA seed sequences abolished the repression of reporter activity. Overexpression of miR-602 or miR-608 inhibited the expression of SHH mRNA and protein, and this was abrogated by antagomirs. Stimulation with recombinant human SHH protein up-regulated MMP-13 expression, and inhibition of HH signaling blocked MMP-13 expression in OA chondrocytes.MiR-602 and miR-608 are important posttranscription regulators of SHH expression in OA chondrocytes, and their suppression by IL-1β may contribute to the enhanced expression of SHH and MMP-13 in OA." @default.
W2114393976 created "2016-06-24" @default.
W2114393976 creator A5022360851 @default.
W2114393976 creator A5037021649 @default.
W2114393976 creator A5057245262 @default.
W2114393976 date "2015-01-28" @default.
W2114393976 modified "2023-10-12" @default.
W2114393976 title "MicroRNA‐602 and MicroRNA‐608 Regulate Sonic Hedgehog Expression via Target Sites in the Coding Region in Human Chondrocytes" @default.
W2114393976 cites W144423133 @default.
W2114393976 cites W1511680446 @default.
W2114393976 cites W1785629757 @default.
W2114393976 cites W1982163159 @default.
W2114393976 cites W1983460322 @default.
W2114393976 cites W1985612711 @default.
W2114393976 cites W1986370183 @default.
W2114393976 cites W1987226609 @default.
W2114393976 cites W1992461587 @default.
W2114393976 cites W1998666504 @default.
W2114393976 cites W2011227409 @default.
W2114393976 cites W2012774801 @default.
W2114393976 cites W2014028055 @default.
W2114393976 cites W2021387873 @default.
W2114393976 cites W2023967851 @default.
W2114393976 cites W2038773696 @default.
W2114393976 cites W2050177842 @default.
W2114393976 cites W2051654549 @default.
W2114393976 cites W2052994887 @default.
W2114393976 cites W2058147305 @default.
W2114393976 cites W2076419655 @default.
W2114393976 cites W2081733368 @default.
W2114393976 cites W2089233425 @default.
W2114393976 cites W2089605568 @default.
W2114393976 cites W2091167062 @default.
W2114393976 cites W2096388167 @default.
W2114393976 cites W2103890520 @default.
W2114393976 cites W2103917047 @default.
W2114393976 cites W2106582491 @default.
W2114393976 cites W2107552781 @default.
W2114393976 cites W2108707301 @default.
W2114393976 cites W2116099686 @default.
W2114393976 cites W2116799486 @default.
W2114393976 cites W2118335497 @default.
W2114393976 cites W2128652401 @default.
W2114393976 cites W2132345567 @default.
W2114393976 cites W2146571398 @default.
W2114393976 cites W2152217106 @default.
W2114393976 cites W2156512494 @default.
W2114393976 cites W2157936237 @default.
W2114393976 cites W2159325140 @default.
W2114393976 cites W2162406636 @default.
W2114393976 cites W2314044888 @default.
W2114393976 cites W4248592944 @default.
W2114393976 doi "https://doi.org/10.1002/art.38952" @default.
W2114393976 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4539020" @default.
W2114393976 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25385442" @default.
W2114393976 hasPublicationYear "2015" @default.
W2114393976 type Work @default.
W2114393976 sameAs 2114393976 @default.
W2114393976 citedByCount "61" @default.
W2114393976 countsByYear W21143939762015 @default.
W2114393976 countsByYear W21143939762016 @default.
W2114393976 countsByYear W21143939762017 @default.
W2114393976 countsByYear W21143939762018 @default.
W2114393976 countsByYear W21143939762019 @default.
W2114393976 countsByYear W21143939762020 @default.
W2114393976 countsByYear W21143939762021 @default.
W2114393976 countsByYear W21143939762022 @default.
W2114393976 countsByYear W21143939762023 @default.
W2114393976 crossrefType "journal-article" @default.
W2114393976 hasAuthorship W2114393976A5022360851 @default.
W2114393976 hasAuthorship W2114393976A5037021649 @default.
W2114393976 hasAuthorship W2114393976A5057245262 @default.
W2114393976 hasBestOaLocation W21143939762 @default.
W2114393976 hasConcept C104317684 @default.
W2114393976 hasConcept C105580179 @default.
W2114393976 hasConcept C105702510 @default.
W2114393976 hasConcept C145059251 @default.
W2114393976 hasConcept C145837895 @default.
W2114393976 hasConcept C150194340 @default.
W2114393976 hasConcept C153911025 @default.
W2114393976 hasConcept C161733203 @default.
W2114393976 hasConcept C174510640 @default.
W2114393976 hasConcept C2776637008 @default.
W2114393976 hasConcept C2778539099 @default.
W2114393976 hasConcept C2780550940 @default.
W2114393976 hasConcept C2781403057 @default.
W2114393976 hasConcept C54009773 @default.
W2114393976 hasConcept C55493867 @default.
W2114393976 hasConcept C62478195 @default.
W2114393976 hasConcept C86803240 @default.
W2114393976 hasConcept C88498014 @default.
W2114393976 hasConcept C95444343 @default.
W2114393976 hasConceptScore W2114393976C104317684 @default.
W2114393976 hasConceptScore W2114393976C105580179 @default.
W2114393976 hasConceptScore W2114393976C105702510 @default.
W2114393976 hasConceptScore W2114393976C145059251 @default.
W2114393976 hasConceptScore W2114393976C145837895 @default.
W2114393976 hasConceptScore W2114393976C150194340 @default.