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- W2114459943 abstract "In this study, the effects of capsaicin on the immunohistochemical distribution and gene expression of transforming growth factor alpha (TGF-alpha) in ovarian tissue of rats at the pubertal age were examined. Materials and methods: Rats (50 days old) were divided into experimental, alimentation, and sham groups. The experimental group was injected with 1 mg/kg doses of capsaicin (10% ethanol and 1% Tween in an 80% distilled water mixture) subcutaneously every day for a period of 1 week. The alimentation group received 1 mg/kg doses of capsaicin (10% ethanol and 1% Tween in an 80% distilled water mixture). The sham group was injected with a solution of 10% ethanol and 1% Tween in a mixture of 80% distilled water. Body weights and ovary weights were compared within the groups. Triple staining methods were used for the histological examinations. The tissue localization of TGF-alpha was analyzed using an immunohistochemical method and gene expression level was determined using the reverse transcriptase-polymerase chain reaction (RT-PCR) technique. Results: No difference in the structure of the 3 groups was observed histologically. Immunohistochemically, TGF-alpha immunoreactivity was detected in the primordial follicles, primary follicles, theca cells, and interstitial cells at a medium density in all groups. The effect of capsaicin on TGF-alpha with mRNA was not found to be significantly different among the 3 groups. Conclusion: The results showed that capsaicin had no effects on ovaries at different developmental stages." @default.
- W2114459943 created "2016-06-24" @default.
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- W2114459943 date "2013-01-01" @default.
- W2114459943 modified "2023-09-25" @default.
- W2114459943 title "Immunohistochemical distribution and gene expression of transforming growth factor alpha in ovarian tissue of rats treated with capsaicin in puberty" @default.
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- W2114459943 doi "https://doi.org/10.3906/sag-1205-120" @default.
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