Matches in SemOpenAlex for { <https://semopenalex.org/work/W2114514993> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2114514993 endingPage "268" @default.
- W2114514993 startingPage "261" @default.
- W2114514993 abstract "Cell sorting and cytotoxic depletion procedures were used to subdivide the population of CD4- CD8- (double-negative) thymocytes from adult CBA mice on the basis of expression of Ly-1, HSA (the heat-stable antigen M1/69 or B2A2), Pgp-1 glycoprotein, Thy-1, MEL-14 and the PC61 antigenic determinant on the IL2 receptor (IL2R). The level of dividing cells within these subsets was assessed by brief in vivo administration of [3H]-thymidine, followed by radioautography, or by flow cytometric cell cycle analysis after DNA staining. The capacity of the subsets to proliferate in culture, in response to stimulation with concanavalin A (Con A), or with phorbol myristate acetate (PMA) and the calcium ionophore ionomycin, was assessed in high cloning efficiency single-cell culture systems. In general, the proliferative response in culture was inversely related to the rate of cell division in vivo. Response of the double-negative subsets to Con A correlated with expression of the T cell antigen receptor complex; although a high cloning efficiency was obtained from the receptor-positive fractions, very few of the clones were cytotoxic. In particular, a major Ly-1+ HSA- Pgp-1+ double-negative subset, as well as minor Ly-1- HSA- Pgp-1+ subsets, contained very few cells in cycle in vivo, but showed a high cloning efficiency in both culture systems. Conversely, the other major double-negative subset, Ly-1- HSA+ Pgp-1-, included most of the cells in cycle, but showed a reduced cloning efficiency in response to PMA and ionomycin and failed to respond to Con A. The dividing cells within the Ly-1- HSA+ Pgp-1- group were strongly enriched in the IL2R- rather than in the IL2R+ subset, suggesting IL2 was not the growth factor maintaining their proliferation in vivo." @default.
- W2114514993 created "2016-06-24" @default.
- W2114514993 creator A5029150372 @default.
- W2114514993 creator A5031157327 @default.
- W2114514993 creator A5062144279 @default.
- W2114514993 creator A5063329786 @default.
- W2114514993 creator A5064135515 @default.
- W2114514993 date "1988-02-01" @default.
- W2114514993 modified "2023-10-14" @default.
- W2114514993 title "Subpopulations of CD4-CD8-murine thymocytes: differences in proliferation rate in vivo and proliferative responses in vitro" @default.
- W2114514993 cites W1999817097 @default.
- W2114514993 cites W2001035036 @default.
- W2114514993 cites W2005291107 @default.
- W2114514993 cites W2015968307 @default.
- W2114514993 cites W2019513091 @default.
- W2114514993 cites W2035397258 @default.
- W2114514993 cites W2037155025 @default.
- W2114514993 cites W2038879439 @default.
- W2114514993 cites W2044001333 @default.
- W2114514993 cites W2061140050 @default.
- W2114514993 cites W2062766097 @default.
- W2114514993 cites W2127598081 @default.
- W2114514993 cites W2162032607 @default.
- W2114514993 doi "https://doi.org/10.1002/eji.1830180213" @default.
- W2114514993 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2894996" @default.
- W2114514993 hasPublicationYear "1988" @default.
- W2114514993 type Work @default.
- W2114514993 sameAs 2114514993 @default.
- W2114514993 citedByCount "32" @default.
- W2114514993 countsByYear W21145149932013 @default.
- W2114514993 countsByYear W21145149932023 @default.
- W2114514993 crossrefType "journal-article" @default.
- W2114514993 hasAuthorship W2114514993A5029150372 @default.
- W2114514993 hasAuthorship W2114514993A5031157327 @default.
- W2114514993 hasAuthorship W2114514993A5062144279 @default.
- W2114514993 hasAuthorship W2114514993A5063329786 @default.
- W2114514993 hasAuthorship W2114514993A5064135515 @default.
- W2114514993 hasConcept C144024400 @default.
- W2114514993 hasConcept C147483822 @default.
- W2114514993 hasConcept C149923435 @default.
- W2114514993 hasConcept C150903083 @default.
- W2114514993 hasConcept C153911025 @default.
- W2114514993 hasConcept C154317977 @default.
- W2114514993 hasConcept C167672396 @default.
- W2114514993 hasConcept C19869320 @default.
- W2114514993 hasConcept C202751555 @default.
- W2114514993 hasConcept C203014093 @default.
- W2114514993 hasConcept C207001950 @default.
- W2114514993 hasConcept C2776090121 @default.
- W2114514993 hasConcept C2776989580 @default.
- W2114514993 hasConcept C2908647359 @default.
- W2114514993 hasConcept C2992881475 @default.
- W2114514993 hasConcept C55493867 @default.
- W2114514993 hasConcept C86803240 @default.
- W2114514993 hasConcept C8891405 @default.
- W2114514993 hasConceptScore W2114514993C144024400 @default.
- W2114514993 hasConceptScore W2114514993C147483822 @default.
- W2114514993 hasConceptScore W2114514993C149923435 @default.
- W2114514993 hasConceptScore W2114514993C150903083 @default.
- W2114514993 hasConceptScore W2114514993C153911025 @default.
- W2114514993 hasConceptScore W2114514993C154317977 @default.
- W2114514993 hasConceptScore W2114514993C167672396 @default.
- W2114514993 hasConceptScore W2114514993C19869320 @default.
- W2114514993 hasConceptScore W2114514993C202751555 @default.
- W2114514993 hasConceptScore W2114514993C203014093 @default.
- W2114514993 hasConceptScore W2114514993C207001950 @default.
- W2114514993 hasConceptScore W2114514993C2776090121 @default.
- W2114514993 hasConceptScore W2114514993C2776989580 @default.
- W2114514993 hasConceptScore W2114514993C2908647359 @default.
- W2114514993 hasConceptScore W2114514993C2992881475 @default.
- W2114514993 hasConceptScore W2114514993C55493867 @default.
- W2114514993 hasConceptScore W2114514993C86803240 @default.
- W2114514993 hasConceptScore W2114514993C8891405 @default.
- W2114514993 hasIssue "2" @default.
- W2114514993 hasLocation W21145149931 @default.
- W2114514993 hasLocation W21145149932 @default.
- W2114514993 hasOpenAccess W2114514993 @default.
- W2114514993 hasPrimaryLocation W21145149931 @default.
- W2114514993 hasRelatedWork W1505145311 @default.
- W2114514993 hasRelatedWork W1513178816 @default.
- W2114514993 hasRelatedWork W1987331268 @default.
- W2114514993 hasRelatedWork W2008949385 @default.
- W2114514993 hasRelatedWork W2018916009 @default.
- W2114514993 hasRelatedWork W2054101033 @default.
- W2114514993 hasRelatedWork W2089075738 @default.
- W2114514993 hasRelatedWork W2164750677 @default.
- W2114514993 hasRelatedWork W2417036897 @default.
- W2114514993 hasRelatedWork W2182050838 @default.
- W2114514993 hasVolume "18" @default.
- W2114514993 isParatext "false" @default.
- W2114514993 isRetracted "false" @default.
- W2114514993 magId "2114514993" @default.
- W2114514993 workType "article" @default.