FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2114523902> ?p ?o ?g. }

• W2114523902 endingPage "31" @default.
• W2114523902 startingPage "23" @default.
• W2114523902 abstract "Abstract Introduction Leonurus sibiricus L. is regularly used in traditional Mongolian medicine including for the treatment of symptoms of diabetes mellitus. Objectives To provide a validated quantitation method for the quality control of Leonurus sibiricus and to prove in vitro insulin‐sensitisation, thereby supporting the traditional use of Leonurus sibiricus . Methodology Pulverised Leonurus sibiricus material was either extracted with methanol or methanol:water (25:75, v / v ). HPLC‐CAD (charged aerosol detector) separations were performed on a Luna Phenyl‐Hexyl column with water and acetonitrile (both modified with 0.1% formic acid) as mobile phase. Gradient elution was employed using theophylline as internal standard. Tentative peak identification was facilitated by HPLC‐MS. Validation was carried out according to ICH (International Conference on Harmonisation) guidelines. Potential insulin‐sensitisation of accordant extracts was assessed in glucose uptake experiments in C2C12 myocytes and protein tyrosine phosphatase 1B (PTP1B) enzyme assays. Results Thirty‐six compounds were tentatively identified based on their retention times, UV spectra, MS fragments and data from literature. They comprise phenolcarboxylic acids, flavonoids, iridoid glycosides, and phenylpropanoids, among which acetylharpagide, ajugoside, lavandulifolioside, and verbascoside were selected for quantitation. The methanol extract contained 0.42% combined iridoids, and 1.58% combined phenylpropanoids. Validation showed good accuracy, intermediate precision and robustness. The methanol extract of Leonurus sibiricus led to a 1.5 fold increase in insulin‐stimulated cellular glucose uptake and inhibition of PTP1B by 40% at a concentration of 10 µg/mL. Conclusion HPLC‐CAD analysis allowed sensitive quantitation of the selected marker compounds in Leonurus sibiricus , thereby providing a reliable tool for its quality control. Copyright © 2015 John Wiley & Sons, Ltd." @default.
• W2114523902 created "2016-06-24" @default.
• W2114523902 creator A5002164427 @default.
• W2114523902 creator A5007899489 @default.
• W2114523902 creator A5028937904 @default.
• W2114523902 creator A5052003385 @default.
• W2114523902 creator A5070340122 @default.
• W2114523902 creator A5084218913 @default.
• W2114523902 creator A5086995430 @default.
• W2114523902 date "2015-09-02" @default.
• W2114523902 modified "2023-10-16" @default.
• W2114523902 title "Quantitation of phenylpropanoids and iridoids in insulin-sensitising extracts of <i>Leonurus sibiricus</i> L. (Lamiaceae)" @default.
• W2114523902 cites W1540942663 @default.
• W2114523902 cites W1981793995 @default.
• W2114523902 cites W2005397735 @default.
• W2114523902 cites W2023244415 @default.
• W2114523902 cites W2040917322 @default.
• W2114523902 cites W2050305665 @default.
• W2114523902 cites W2070946881 @default.
• W2114523902 cites W2082012564 @default.
• W2114523902 cites W2086911046 @default.
• W2114523902 cites W2144327106 @default.
• W2114523902 cites W2168823172 @default.
• W2114523902 cites W2170040165 @default.
• W2114523902 doi "https://doi.org/10.1002/pca.2583" @default.
• W2114523902 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26333151" @default.
• W2114523902 hasPublicationYear "2015" @default.
• W2114523902 type Work @default.
• W2114523902 sameAs 2114523902 @default.
• W2114523902 citedByCount "19" @default.
• W2114523902 countsByYear W21145239022016 @default.
• W2114523902 countsByYear W21145239022017 @default.
• W2114523902 countsByYear W21145239022019 @default.
• W2114523902 countsByYear W21145239022020 @default.
• W2114523902 countsByYear W21145239022021 @default.
• W2114523902 countsByYear W21145239022022 @default.
• W2114523902 crossrefType "journal-article" @default.
• W2114523902 hasAuthorship W2114523902A5002164427 @default.
• W2114523902 hasAuthorship W2114523902A5007899489 @default.
• W2114523902 hasAuthorship W2114523902A5028937904 @default.
• W2114523902 hasAuthorship W2114523902A5052003385 @default.
• W2114523902 hasAuthorship W2114523902A5070340122 @default.
• W2114523902 hasAuthorship W2114523902A5084218913 @default.
• W2114523902 hasAuthorship W2114523902A5086995430 @default.
• W2114523902 hasConcept C142724271 @default.
• W2114523902 hasConcept C178790620 @default.
• W2114523902 hasConcept C179998833 @default.
• W2114523902 hasConcept C185592680 @default.
• W2114523902 hasConcept C194671627 @default.
• W2114523902 hasConcept C204787440 @default.
• W2114523902 hasConcept C2777379063 @default.
• W2114523902 hasConcept C2778413156 @default.
• W2114523902 hasConcept C2779607525 @default.
• W2114523902 hasConcept C2780439029 @default.
• W2114523902 hasConcept C2781347565 @default.
• W2114523902 hasConcept C43617362 @default.
• W2114523902 hasConcept C71240020 @default.
• W2114523902 hasConcept C71924100 @default.
• W2114523902 hasConceptScore W2114523902C142724271 @default.
• W2114523902 hasConceptScore W2114523902C178790620 @default.
• W2114523902 hasConceptScore W2114523902C179998833 @default.
• W2114523902 hasConceptScore W2114523902C185592680 @default.
• W2114523902 hasConceptScore W2114523902C194671627 @default.
• W2114523902 hasConceptScore W2114523902C204787440 @default.
• W2114523902 hasConceptScore W2114523902C2777379063 @default.
• W2114523902 hasConceptScore W2114523902C2778413156 @default.
• W2114523902 hasConceptScore W2114523902C2779607525 @default.
• W2114523902 hasConceptScore W2114523902C2780439029 @default.
• W2114523902 hasConceptScore W2114523902C2781347565 @default.
• W2114523902 hasConceptScore W2114523902C43617362 @default.
• W2114523902 hasConceptScore W2114523902C71240020 @default.
• W2114523902 hasConceptScore W2114523902C71924100 @default.
• W2114523902 hasFunder F4320324069 @default.
• W2114523902 hasIssue "1" @default.
• W2114523902 hasLocation W21145239021 @default.
• W2114523902 hasLocation W21145239022 @default.
• W2114523902 hasOpenAccess W2114523902 @default.
• W2114523902 hasPrimaryLocation W21145239021 @default.
• W2114523902 hasRelatedWork W1968618570 @default.
• W2114523902 hasRelatedWork W1973456085 @default.
• W2114523902 hasRelatedWork W2020294797 @default.
• W2114523902 hasRelatedWork W2024609460 @default.
• W2114523902 hasRelatedWork W2033611131 @default.
• W2114523902 hasRelatedWork W2036494804 @default.
• W2114523902 hasRelatedWork W2093161064 @default.
• W2114523902 hasRelatedWork W4245109017 @default.
• W2114523902 hasRelatedWork W4285480625 @default.
• W2114523902 hasRelatedWork W2100219638 @default.
• W2114523902 hasVolume "27" @default.
• W2114523902 isParatext "false" @default.
• W2114523902 isRetracted "false" @default.
• W2114523902 magId "2114523902" @default.
• W2114523902 workType "article" @default.