Matches in SemOpenAlex for { <https://semopenalex.org/work/W2114624178> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2114624178 endingPage "7462" @default.
- W2114624178 startingPage "7457" @default.
- W2114624178 abstract "ABSTRACT Aminotransferases (ATs) interacting with l -alanine are the least studied bacterial ATs. Whereas AlaT converts pyruvate to l -alanine in a glutamate-dependent reaction, AvtA is able to convert pyruvate to l -alanine in an l -valine-dependent manner. We show here that the wild type of Corynebacterium glutamicum with a deletion of either of the corresponding genes does not exhibit an explicit growth deficiency. However, a double mutant was auxotrophic for l -alanine, showing that both ATs can provide l -alanine and that they are the only ATs involved. Kinetic studies with isolated enzymes demonstrate that the catalytic efficiency, k cat / K m , of AlaT is higher than 1 order of magnitude in the direction of l -alanine formation (3.5 × 10 4 M −1 s −1 ), but no preference was apparent for AvtA, suggesting that AlaT is the principal l -alanine-supplying enzyme. This is in line with the cytosolic l -alanine concentration, which is reduced in the exponential growth phase from 95 mM to 18 mM by a deletion of alaT , whereas avtA deletion decreases the l -alanine concentration only to 76 mM. The combined data show that the presence of both ATs has subtle but obvious consequences on balancing intracellular amino acid pools in the wild type. The consequences are more obvious in an l -valine production strain where a high intracellular drain-off of the l -alanine precursor pyruvate prevails. We therefore used deletion of alaT to successfully reduce the contaminating l -alanine in extracellular accumulated l -valine by 80%." @default.
- W2114624178 created "2016-06-24" @default.
- W2114624178 creator A5027890414 @default.
- W2114624178 creator A5085419424 @default.
- W2114624178 date "2008-12-15" @default.
- W2114624178 modified "2023-10-07" @default.
- W2114624178 title "Metabolic Function of <i>Corynebacterium glutamicum</i> Aminotransferases AlaT and AvtA and Impact on <scp>l</scp> -Valine Production" @default.
- W2114624178 cites W1487753561 @default.
- W2114624178 cites W1500652345 @default.
- W2114624178 cites W1504884169 @default.
- W2114624178 cites W1508709132 @default.
- W2114624178 cites W1550383119 @default.
- W2114624178 cites W1847296879 @default.
- W2114624178 cites W1878018588 @default.
- W2114624178 cites W1905714928 @default.
- W2114624178 cites W1971064600 @default.
- W2114624178 cites W1984345785 @default.
- W2114624178 cites W2019796789 @default.
- W2114624178 cites W2020631324 @default.
- W2114624178 cites W2030463997 @default.
- W2114624178 cites W2038663142 @default.
- W2114624178 cites W2055628150 @default.
- W2114624178 cites W2059352446 @default.
- W2114624178 cites W2072278470 @default.
- W2114624178 cites W2089041533 @default.
- W2114624178 cites W2090959570 @default.
- W2114624178 cites W2092727311 @default.
- W2114624178 cites W2115955917 @default.
- W2114624178 cites W2131240172 @default.
- W2114624178 cites W2139424846 @default.
- W2114624178 cites W2145213365 @default.
- W2114624178 cites W2153076508 @default.
- W2114624178 cites W2158195451 @default.
- W2114624178 cites W4248664566 @default.
- W2114624178 doi "https://doi.org/10.1128/aem.01025-08" @default.
- W2114624178 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2607172" @default.
- W2114624178 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18931286" @default.
- W2114624178 hasPublicationYear "2008" @default.
- W2114624178 type Work @default.
- W2114624178 sameAs 2114624178 @default.
- W2114624178 citedByCount "54" @default.
- W2114624178 countsByYear W21146241782012 @default.
- W2114624178 countsByYear W21146241782013 @default.
- W2114624178 countsByYear W21146241782014 @default.
- W2114624178 countsByYear W21146241782015 @default.
- W2114624178 countsByYear W21146241782016 @default.
- W2114624178 countsByYear W21146241782017 @default.
- W2114624178 countsByYear W21146241782018 @default.
- W2114624178 countsByYear W21146241782019 @default.
- W2114624178 countsByYear W21146241782020 @default.
- W2114624178 countsByYear W21146241782021 @default.
- W2114624178 countsByYear W21146241782023 @default.
- W2114624178 crossrefType "journal-article" @default.
- W2114624178 hasAuthorship W2114624178A5027890414 @default.
- W2114624178 hasAuthorship W2114624178A5085419424 @default.
- W2114624178 hasBestOaLocation W21146241781 @default.
- W2114624178 hasConcept C104317684 @default.
- W2114624178 hasConcept C143065580 @default.
- W2114624178 hasConcept C185592680 @default.
- W2114624178 hasConcept C20950039 @default.
- W2114624178 hasConcept C2776260651 @default.
- W2114624178 hasConcept C2777573094 @default.
- W2114624178 hasConcept C2779856020 @default.
- W2114624178 hasConcept C515207424 @default.
- W2114624178 hasConcept C55493867 @default.
- W2114624178 hasConcept C86803240 @default.
- W2114624178 hasConceptScore W2114624178C104317684 @default.
- W2114624178 hasConceptScore W2114624178C143065580 @default.
- W2114624178 hasConceptScore W2114624178C185592680 @default.
- W2114624178 hasConceptScore W2114624178C20950039 @default.
- W2114624178 hasConceptScore W2114624178C2776260651 @default.
- W2114624178 hasConceptScore W2114624178C2777573094 @default.
- W2114624178 hasConceptScore W2114624178C2779856020 @default.
- W2114624178 hasConceptScore W2114624178C515207424 @default.
- W2114624178 hasConceptScore W2114624178C55493867 @default.
- W2114624178 hasConceptScore W2114624178C86803240 @default.
- W2114624178 hasIssue "24" @default.
- W2114624178 hasLocation W21146241781 @default.
- W2114624178 hasLocation W21146241782 @default.
- W2114624178 hasLocation W21146241783 @default.
- W2114624178 hasLocation W21146241784 @default.
- W2114624178 hasOpenAccess W2114624178 @default.
- W2114624178 hasPrimaryLocation W21146241781 @default.
- W2114624178 hasRelatedWork W1983804884 @default.
- W2114624178 hasRelatedWork W2020631324 @default.
- W2114624178 hasRelatedWork W2045859423 @default.
- W2114624178 hasRelatedWork W2052955587 @default.
- W2114624178 hasRelatedWork W2055669739 @default.
- W2114624178 hasRelatedWork W2074663367 @default.
- W2114624178 hasRelatedWork W2382497309 @default.
- W2114624178 hasRelatedWork W2948341197 @default.
- W2114624178 hasRelatedWork W4206983360 @default.
- W2114624178 hasRelatedWork W4289754849 @default.
- W2114624178 hasVolume "74" @default.
- W2114624178 isParatext "false" @default.
- W2114624178 isRetracted "false" @default.
- W2114624178 magId "2114624178" @default.
- W2114624178 workType "article" @default.