Matches in SemOpenAlex for { <https://semopenalex.org/work/W2114633404> ?p ?o ?g. }
- W2114633404 endingPage "918" @default.
- W2114633404 startingPage "912" @default.
- W2114633404 abstract "Simian virus 40 T antigen (TAg) exhibits nonspecific and origin-specific DNA binding (ori binding) and ATPase and helicase activities, all of which are related to its roles in viral DNA replication. We have characterized some of the properties of four replication-defective but transformation-competent mutant TAgs, C6-2, T22, C11, and C8A. C6-2 and T22 TAgs were each previously determined to lack ori-binding properties, while C11 TAg was reported to lack ATPase activity. The C8A TAg did not exhibit defects in either ori-binding or ATPase functions. We have analyzed additional aspects of these mutant TAgs pertaining to their helicase, DNA-binding, and immunological properties. With the exception of the C11 TAg, all the other TAgs exhibited helicase activity. The lack of helicase activity by C11 TAg was consistent with its previously shown inability to hydrolyze ATP or to replicate viral DNA. These results therefore show that ori-binding and helicase activities are separate functions of TAg. Wild-type and mutant TAgs bound with similar efficiency to either native or denatured calf thymus DNA-cellulose, indicating no marked differences in their nonspecific DNA-binding properties. We also tested the binding of wild-type and mutant TAgs to a monoclonal antibody, PAb 100, that was previously shown to recognize an extremely small class of TAg that may represent a unique conformational form of the protein. Interestingly, while less than 10% of the wild-type, C6-2, C11, and T22 mutant TAgs were recognized by PAb 100, more than 60% of the C8A mutant TAg was bound by this antibody. Therefore, although no defect in biochemical function was observed with the C8A TAg, its deficiency in viral DNA replication may be related to an unusual conformation, as detected by its dramatically increased recognition by PAb 100. These results show that the helicase activity of TAg is not required for its transformation function." @default.
- W2114633404 created "2016-06-24" @default.
- W2114633404 creator A5017182319 @default.
- W2114633404 creator A5059190769 @default.
- W2114633404 creator A5080267048 @default.
- W2114633404 date "1989-02-01" @default.
- W2114633404 modified "2023-09-24" @default.
- W2114633404 title "Helicase, DNA-binding, and immunological properties of replication-defective simian virus 40 mutant T antigens" @default.
- W2114633404 cites W112111172 @default.
- W2114633404 cites W1482051809 @default.
- W2114633404 cites W1522917905 @default.
- W2114633404 cites W1542007689 @default.
- W2114633404 cites W1546632589 @default.
- W2114633404 cites W1565314684 @default.
- W2114633404 cites W1568744107 @default.
- W2114633404 cites W1571850698 @default.
- W2114633404 cites W1594566711 @default.
- W2114633404 cites W1665387657 @default.
- W2114633404 cites W175043000 @default.
- W2114633404 cites W1851443011 @default.
- W2114633404 cites W1856881370 @default.
- W2114633404 cites W1874646775 @default.
- W2114633404 cites W1892859196 @default.
- W2114633404 cites W1899811795 @default.
- W2114633404 cites W1912533594 @default.
- W2114633404 cites W1921957534 @default.
- W2114633404 cites W1934622753 @default.
- W2114633404 cites W1966340370 @default.
- W2114633404 cites W1968010633 @default.
- W2114633404 cites W1980198249 @default.
- W2114633404 cites W1981943850 @default.
- W2114633404 cites W1988202882 @default.
- W2114633404 cites W1991508530 @default.
- W2114633404 cites W2027697827 @default.
- W2114633404 cites W2031125682 @default.
- W2114633404 cites W2031875867 @default.
- W2114633404 cites W2039653473 @default.
- W2114633404 cites W2040412368 @default.
- W2114633404 cites W2050142983 @default.
- W2114633404 cites W2064204579 @default.
- W2114633404 cites W2067419418 @default.
- W2114633404 cites W2105386435 @default.
- W2114633404 cites W2105967953 @default.
- W2114633404 cites W2125026993 @default.
- W2114633404 cites W2125695327 @default.
- W2114633404 cites W2134871100 @default.
- W2114633404 cites W2135890914 @default.
- W2114633404 cites W2139768549 @default.
- W2114633404 cites W2167489116 @default.
- W2114633404 cites W2208707498 @default.
- W2114633404 cites W2291923656 @default.
- W2114633404 cites W2471675115 @default.
- W2114633404 cites W4253280851 @default.
- W2114633404 cites W93885486 @default.
- W2114633404 doi "https://doi.org/10.1128/jvi.63.2.912-918.1989" @default.
- W2114633404 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/247765" @default.
- W2114633404 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2536112" @default.
- W2114633404 hasPublicationYear "1989" @default.
- W2114633404 type Work @default.
- W2114633404 sameAs 2114633404 @default.
- W2114633404 citedByCount "24" @default.
- W2114633404 countsByYear W21146334042016 @default.
- W2114633404 crossrefType "journal-article" @default.
- W2114633404 hasAuthorship W2114633404A5017182319 @default.
- W2114633404 hasAuthorship W2114633404A5059190769 @default.
- W2114633404 hasAuthorship W2114633404A5080267048 @default.
- W2114633404 hasBestOaLocation W21146334041 @default.
- W2114633404 hasConcept C104317684 @default.
- W2114633404 hasConcept C107824862 @default.
- W2114633404 hasConcept C140704245 @default.
- W2114633404 hasConcept C143065580 @default.
- W2114633404 hasConcept C153911025 @default.
- W2114633404 hasConcept C159047783 @default.
- W2114633404 hasConcept C161223559 @default.
- W2114633404 hasConcept C181199279 @default.
- W2114633404 hasConcept C23265538 @default.
- W2114633404 hasConcept C2522874641 @default.
- W2114633404 hasConcept C552990157 @default.
- W2114633404 hasConcept C55493867 @default.
- W2114633404 hasConcept C67705224 @default.
- W2114633404 hasConcept C68592252 @default.
- W2114633404 hasConcept C73573662 @default.
- W2114633404 hasConcept C77400797 @default.
- W2114633404 hasConcept C86339819 @default.
- W2114633404 hasConcept C86803240 @default.
- W2114633404 hasConcept C94966510 @default.
- W2114633404 hasConceptScore W2114633404C104317684 @default.
- W2114633404 hasConceptScore W2114633404C107824862 @default.
- W2114633404 hasConceptScore W2114633404C140704245 @default.
- W2114633404 hasConceptScore W2114633404C143065580 @default.
- W2114633404 hasConceptScore W2114633404C153911025 @default.
- W2114633404 hasConceptScore W2114633404C159047783 @default.
- W2114633404 hasConceptScore W2114633404C161223559 @default.
- W2114633404 hasConceptScore W2114633404C181199279 @default.
- W2114633404 hasConceptScore W2114633404C23265538 @default.
- W2114633404 hasConceptScore W2114633404C2522874641 @default.
- W2114633404 hasConceptScore W2114633404C552990157 @default.
- W2114633404 hasConceptScore W2114633404C55493867 @default.