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- W2114639573 abstract "The development of luminal organs begins with the formation of spherical cysts composed of a single layer of epithelial cells. Using a model three-dimensional cell culture, this study examines the role of a cytoskeletal motor, myosin II, in cyst formation. Caco-2 and SK-CO15 intestinal epithelial cells were embedded into Matrigel, and myosin II was inhibited by blebbistatin or siRNA-mediated knockdown. Whereas control cells formed spherical cysts with a smooth surface, inhibition of myosin II induced the outgrowth of F-actin-rich surface protrusions. The development of these protrusions was abrogated after inhibition of F-actin polymerization or of phospholipase C (PLC) activity, as well as after overexpression of a dominant-negative ADF/cofilin. Surface protrusions were enriched in microtubules and their formation was prevented by microtubule depolymerization. Myosin II inhibition caused a loss of peripheral F-actin bundles and a submembranous extension of cortical microtubules. Our findings suggest that inhibition of myosin II eliminates the cortical F-actin barrier, allowing microtubules to reach and activate PLC at the plasma membrane. PLC-dependent stimulation of ADF/cofilin creates actin-filament barbed ends and promotes the outgrowth of F-actin-rich protrusions. We conclude that myosin II regulates the spherical shape of epithelial cysts by controlling actin polymerization at the cyst surface." @default.
- W2114639573 created "2016-06-24" @default.
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- W2114639573 date "2008-06-01" @default.
- W2114639573 modified "2023-10-18" @default.
- W2114639573 title "Myosin II regulates the shape of three-dimensional intestinal epithelial cysts" @default.
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- W2114639573 doi "https://doi.org/10.1242/jcs.015842" @default.
- W2114639573 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18460584" @default.
- W2114639573 hasPublicationYear "2008" @default.
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