FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2114835737> ?p ?o ?g. }

• W2114835737 endingPage "44" @default.
• W2114835737 startingPage "34" @default.
• W2114835737 abstract "Burkholderia pseudomallei, the etiologic agent of melioidosis, is responsible for a broad spectrum of illnesses in humans and animals particularly in Southeast Asia and northern Australia, where it is endemic. Burkholderia thailandensis is a nonpathogenic environmental organism closely related to B. pseudomallei. Subtractive hybridization was carried out between these two species to identify genes encoding virulence determinants in B. pseudomallei. Screening of the subtraction library revealed A-T-rich DNA sequences unique to B. pseudomallei, suggesting they may have been acquired by horizontal transfer. One of the subtraction clones, pDD1015, encoded a protein with homology to a glycosyltransferase from Pseudomonas aeruginosa. This gene was insertionally inactivated in wild-type B. pseudomallei to create SR1015. It was determined by enzyme-linked immunosorbent assay and immunoelectron microscopy that the inactivated gene was involved in the production of a major surface polysaccharide. The 50% lethal dose (LD(50)) for wild-type B. pseudomallei is <10 CFU; the LD(50) for SR1015 was determined to be 3.5 x 10(5) CFU, similar to that of B. thailandensis (6.8 x 10(5) CFU). DNA sequencing of the region flanking the glycosyltransferase gene revealed open reading frames similar to capsular polysaccharide genes in Haemophilus influenzae, Escherichia coli, and Neisseria meningitidis. In addition, DNA from Burkholderia mallei and Burkholderia stabilis hybridized to a glycosyltransferase fragment probe, and a capsular structure was identified on the surface of B. stabilis via immunoelectron microscopy. Thus, the combination of PCR-based subtractive hybridization, insertional inactivation, and animal virulence studies has facilitated the identification of an important virulence determinant in B. pseudomallei." @default.
• W2114835737 created "2016-06-24" @default.
• W2114835737 creator A5000934588 @default.
• W2114835737 creator A5047031403 @default.
• W2114835737 creator A5051585692 @default.
• W2114835737 creator A5073886576 @default.
• W2114835737 date "2001-01-01" @default.
• W2114835737 modified "2023-09-26" @default.
• W2114835737 title "Detection of Bacterial Virulence Genes by Subtractive Hybridization: Identification of Capsular Polysaccharide of <i>Burkholderia pseudomallei</i> as a Major Virulence Determinant" @default.
• W2114835737 cites W124504601 @default.
• W2114835737 cites W1498935064 @default.
• W2114835737 cites W1543592544 @default.
• W2114835737 cites W1772749380 @default.
• W2114835737 cites W1787690277 @default.
• W2114835737 cites W1865769860 @default.
• W2114835737 cites W1874905358 @default.
• W2114835737 cites W18987091 @default.
• W2114835737 cites W1929621894 @default.
• W2114835737 cites W1954057403 @default.
• W2114835737 cites W1967604721 @default.
• W2114835737 cites W1974470044 @default.
• W2114835737 cites W1975304761 @default.
• W2114835737 cites W1987020412 @default.
• W2114835737 cites W2003824924 @default.
• W2114835737 cites W2013071065 @default.
• W2114835737 cites W2019333220 @default.
• W2114835737 cites W2020878190 @default.
• W2114835737 cites W2027229485 @default.
• W2114835737 cites W2030188525 @default.
• W2114835737 cites W2033976505 @default.
• W2114835737 cites W2040581338 @default.
• W2114835737 cites W2070903001 @default.
• W2114835737 cites W2074879055 @default.
• W2114835737 cites W2079778372 @default.
• W2114835737 cites W2081247540 @default.
• W2114835737 cites W2082345749 @default.
• W2114835737 cites W2086010696 @default.
• W2114835737 cites W2087606064 @default.
• W2114835737 cites W2088836626 @default.
• W2114835737 cites W2098598894 @default.
• W2114835737 cites W2101891378 @default.
• W2114835737 cites W2102217124 @default.
• W2114835737 cites W2102484565 @default.
• W2114835737 cites W2102558489 @default.
• W2114835737 cites W2103535426 @default.
• W2114835737 cites W2108723412 @default.
• W2114835737 cites W2109889137 @default.
• W2114835737 cites W2109911118 @default.
• W2114835737 cites W2124958702 @default.
• W2114835737 cites W2126006315 @default.
• W2114835737 cites W2126342660 @default.
• W2114835737 cites W2126761720 @default.
• W2114835737 cites W2128639098 @default.
• W2114835737 cites W2129524689 @default.
• W2114835737 cites W2134153749 @default.
• W2114835737 cites W2135261732 @default.
• W2114835737 cites W2137217076 @default.
• W2114835737 cites W2140596577 @default.
• W2114835737 cites W2143110971 @default.
• W2114835737 cites W2155155224 @default.
• W2114835737 cites W2158023121 @default.
• W2114835737 cites W2166034141 @default.
• W2114835737 cites W2171862804 @default.
• W2114835737 cites W2244667908 @default.
• W2114835737 cites W2418866887 @default.
• W2114835737 cites W2486937903 @default.
• W2114835737 cites W4236067310 @default.
• W2114835737 doi "https://doi.org/10.1128/iai.69.1.34-44.2001" @default.
• W2114835737 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/97852" @default.
• W2114835737 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11119486" @default.
• W2114835737 hasPublicationYear "2001" @default.
• W2114835737 type Work @default.
• W2114835737 sameAs 2114835737 @default.
• W2114835737 citedByCount "247" @default.
• W2114835737 countsByYear W21148357372012 @default.
• W2114835737 countsByYear W21148357372013 @default.
• W2114835737 countsByYear W21148357372014 @default.
• W2114835737 countsByYear W21148357372015 @default.
• W2114835737 countsByYear W21148357372016 @default.
• W2114835737 countsByYear W21148357372017 @default.
• W2114835737 countsByYear W21148357372018 @default.
• W2114835737 countsByYear W21148357372019 @default.
• W2114835737 countsByYear W21148357372020 @default.
• W2114835737 countsByYear W21148357372021 @default.
• W2114835737 countsByYear W21148357372022 @default.
• W2114835737 countsByYear W21148357372023 @default.
• W2114835737 crossrefType "journal-article" @default.
• W2114835737 hasAuthorship W2114835737A5000934588 @default.
• W2114835737 hasAuthorship W2114835737A5047031403 @default.
• W2114835737 hasAuthorship W2114835737A5051585692 @default.
• W2114835737 hasAuthorship W2114835737A5073886576 @default.
• W2114835737 hasBestOaLocation W21148357372 @default.
• W2114835737 hasConcept C104317684 @default.
• W2114835737 hasConcept C159654299 @default.
• W2114835737 hasConcept C187882448 @default.
• W2114835737 hasConcept C189482833 @default.
• W2114835737 hasConcept C196436469 @default.
• W2114835737 hasConcept C2779110727 @default.

• next