Matches in SemOpenAlex for { <https://semopenalex.org/work/W2114856472> ?p ?o ?g. }
- W2114856472 endingPage "10196" @default.
- W2114856472 startingPage "10191" @default.
- W2114856472 abstract "The complete DNA sequence of Pseudomonas aeruginosa provides an opportunity to apply functional genomics to a major human pathogen. A comparative genomics approach combined with genetic footprinting was used as a strategy to identify genes required for viability in P. aeruginosa. Use of a highly efficient in vivo mariner transposition system in P. aeruginosa facilitated the analysis of candidate genes of this class. We have developed a rapid and efficient allelic exchange system by using the I-SceI homing endonuclease in conjunction with in vitro mariner mutagenesis to generate mutants within targeted regions of the P. aeruginosa chromosome for genetic footprinting analyses. This technique for generating transposon insertion mutants should be widely applicable to other organisms that are not naturally transformable or may lack well developed in vivo transposition systems. We tested this system with three genes in P. aeruginosa that have putative essential homologs in Haemophilus influenzae. We show that one of three H. influenzae essential gene homologs is needed for growth in P. aeruginosa, validating the practicality of this comparative genomics strategy to identify essential genes in P. aeruginosa." @default.
- W2114856472 created "2016-06-24" @default.
- W2114856472 creator A5045253972 @default.
- W2114856472 creator A5090287874 @default.
- W2114856472 date "2000-08-29" @default.
- W2114856472 modified "2023-10-17" @default.
- W2114856472 title "Genetic footprinting with <i>mariner</i> -based transposition in <i>Pseudomonas</i> <i>aeruginosa</i>" @default.
- W2114856472 cites W1509386929 @default.
- W2114856472 cites W1779180564 @default.
- W2114856472 cites W1834101661 @default.
- W2114856472 cites W1845461494 @default.
- W2114856472 cites W189047847 @default.
- W2114856472 cites W194917975 @default.
- W2114856472 cites W1950503156 @default.
- W2114856472 cites W1969737313 @default.
- W2114856472 cites W1996001643 @default.
- W2114856472 cites W1998348841 @default.
- W2114856472 cites W2005058090 @default.
- W2114856472 cites W2006444960 @default.
- W2114856472 cites W2010763778 @default.
- W2114856472 cites W2013902051 @default.
- W2114856472 cites W2027875921 @default.
- W2114856472 cites W2030959690 @default.
- W2114856472 cites W2033722802 @default.
- W2114856472 cites W2041880715 @default.
- W2114856472 cites W2053096433 @default.
- W2114856472 cites W2061909665 @default.
- W2114856472 cites W2064691604 @default.
- W2114856472 cites W2089086408 @default.
- W2114856472 cites W2131423362 @default.
- W2114856472 cites W2158714788 @default.
- W2114856472 cites W2161767156 @default.
- W2114856472 cites W2164698321 @default.
- W2114856472 cites W2168460045 @default.
- W2114856472 cites W2171315493 @default.
- W2114856472 cites W2314520051 @default.
- W2114856472 cites W4241126358 @default.
- W2114856472 doi "https://doi.org/10.1073/pnas.97.18.10191" @default.
- W2114856472 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/27802" @default.
- W2114856472 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10963681" @default.
- W2114856472 hasPublicationYear "2000" @default.
- W2114856472 type Work @default.
- W2114856472 sameAs 2114856472 @default.
- W2114856472 citedByCount "151" @default.
- W2114856472 countsByYear W21148564722012 @default.
- W2114856472 countsByYear W21148564722013 @default.
- W2114856472 countsByYear W21148564722014 @default.
- W2114856472 countsByYear W21148564722015 @default.
- W2114856472 countsByYear W21148564722016 @default.
- W2114856472 countsByYear W21148564722017 @default.
- W2114856472 countsByYear W21148564722018 @default.
- W2114856472 countsByYear W21148564722019 @default.
- W2114856472 countsByYear W21148564722020 @default.
- W2114856472 countsByYear W21148564722021 @default.
- W2114856472 countsByYear W21148564722022 @default.
- W2114856472 countsByYear W21148564722023 @default.
- W2114856472 crossrefType "journal-article" @default.
- W2114856472 hasAuthorship W2114856472A5045253972 @default.
- W2114856472 hasAuthorship W2114856472A5090287874 @default.
- W2114856472 hasBestOaLocation W21148564722 @default.
- W2114856472 hasConcept C104317684 @default.
- W2114856472 hasConcept C105176652 @default.
- W2114856472 hasConcept C141231307 @default.
- W2114856472 hasConcept C143065580 @default.
- W2114856472 hasConcept C161078062 @default.
- W2114856472 hasConcept C175817850 @default.
- W2114856472 hasConcept C189206191 @default.
- W2114856472 hasConcept C2776925733 @default.
- W2114856472 hasConcept C2777637488 @default.
- W2114856472 hasConcept C28693550 @default.
- W2114856472 hasConcept C4918238 @default.
- W2114856472 hasConcept C523546767 @default.
- W2114856472 hasConcept C54355233 @default.
- W2114856472 hasConcept C86803240 @default.
- W2114856472 hasConceptScore W2114856472C104317684 @default.
- W2114856472 hasConceptScore W2114856472C105176652 @default.
- W2114856472 hasConceptScore W2114856472C141231307 @default.
- W2114856472 hasConceptScore W2114856472C143065580 @default.
- W2114856472 hasConceptScore W2114856472C161078062 @default.
- W2114856472 hasConceptScore W2114856472C175817850 @default.
- W2114856472 hasConceptScore W2114856472C189206191 @default.
- W2114856472 hasConceptScore W2114856472C2776925733 @default.
- W2114856472 hasConceptScore W2114856472C2777637488 @default.
- W2114856472 hasConceptScore W2114856472C28693550 @default.
- W2114856472 hasConceptScore W2114856472C4918238 @default.
- W2114856472 hasConceptScore W2114856472C523546767 @default.
- W2114856472 hasConceptScore W2114856472C54355233 @default.
- W2114856472 hasConceptScore W2114856472C86803240 @default.
- W2114856472 hasIssue "18" @default.
- W2114856472 hasLocation W21148564721 @default.
- W2114856472 hasLocation W21148564722 @default.
- W2114856472 hasLocation W21148564723 @default.
- W2114856472 hasLocation W21148564724 @default.
- W2114856472 hasOpenAccess W2114856472 @default.
- W2114856472 hasPrimaryLocation W21148564721 @default.
- W2114856472 hasRelatedWork W2092829297 @default.
- W2114856472 hasRelatedWork W2116665001 @default.
- W2114856472 hasRelatedWork W2116734196 @default.