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• W2114879969 abstract "To facilitate in vitro studies of the immunology of human leishmaniasis, we developed a method of growing pathogenic Leishmania in human monocyte-derived macrophages. After 6 days of incubation, adherent mononuclear cells were infected with Leishmania donovani amastigotes obtained from infected hamster spleen cells or with L. tropica amastigotes obtained from infected BALB/c tissue mouse footpad. Forty-eight percent of the macrophages were initially infected, with a mean of 3.0 amastigotes per infected macrophage. After 6 days of incubation, 59% of macrophages were infected and contained 8.8 amastigotes per infected macrophage, representing 2.9-fold multiplication. Electron microscopy revealed the presence of dividing parasites within phagolysosomes. These observations indicate that Leishmania survive and multiply within human monocyte-derived macrophages despite fusion of secondary lysosomes with the parasitophorous vacuole." @default.
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• W2114879969 date "1979-10-01" @default.
• W2114879969 modified "2023-10-01" @default.
• W2114879969 title "Multiplication of Leishmania in human macrophages in vitro" @default.
• W2114879969 doi "https://doi.org/10.1128/iai.26.1.375-379.1979" @default.
• W2114879969 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/414622" @default.
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