Matches in SemOpenAlex for { <https://semopenalex.org/work/W2114885153> ?p ?o ?g. }
- W2114885153 endingPage "e80066" @default.
- W2114885153 startingPage "e80066" @default.
- W2114885153 abstract "Background In addition to their general role in the hydrolysis of storage lipids, bumblebee lipases can participate in the biosynthesis of fatty acids that serve as precursors of pheromones used for sexual communication. Results We studied the temporal dynamics of lipolytic activity in crude extracts from the cephalic part of Bombus terrestris labial glands. Extracts from 3-day-old males displayed the highest lipolytic activity. The highest lipase gene expression level was observed in freshly emerged bumblebees, and both gene expression and lipase activity were lower in bumblebees older than 3 days. Lipase was purified from labial glands, further characterized and named as BT-1. The B. terrestris orthologue shares 88% sequence identity with B. impatiens lipase HA. The molecular weight of B. terrestris lipase BT-1 was approximately 30 kDa, the pH optimum was 8.3, and the temperature optimum was 50°C. Lipase BT-1 showed a notable preference for C8-C10 p-nitrophenyl esters, with the highest activity toward p-nitrophenyl caprylate (C8). The Michaelis constant (Km) and maximum reaction rate (Vmax) for p-nitrophenyl laurate hydrolysis were Km = 0.0011 mM and Vmax = 0.15 U/mg. Conclusion This is the first report describing neutral lipase from the labial gland of B. terrestris. Our findings help increase understanding of its possible function in the labial gland." @default.
- W2114885153 created "2016-06-24" @default.
- W2114885153 creator A5001446297 @default.
- W2114885153 creator A5027818166 @default.
- W2114885153 creator A5061841750 @default.
- W2114885153 creator A5065085743 @default.
- W2114885153 creator A5065614485 @default.
- W2114885153 creator A5085760582 @default.
- W2114885153 creator A5089897001 @default.
- W2114885153 creator A5090479609 @default.
- W2114885153 date "2013-11-08" @default.
- W2114885153 modified "2023-10-01" @default.
- W2114885153 title "Characterization of Neutral Lipase BT-1 Isolated from the Labial Gland of Bombus terrestris Males" @default.
- W2114885153 cites W105893388 @default.
- W2114885153 cites W137827648 @default.
- W2114885153 cites W1481296516 @default.
- W2114885153 cites W1526864164 @default.
- W2114885153 cites W1964222798 @default.
- W2114885153 cites W1966115239 @default.
- W2114885153 cites W1966982442 @default.
- W2114885153 cites W1969907084 @default.
- W2114885153 cites W1971012854 @default.
- W2114885153 cites W1974611898 @default.
- W2114885153 cites W1984524364 @default.
- W2114885153 cites W1991349267 @default.
- W2114885153 cites W1992597413 @default.
- W2114885153 cites W1993010945 @default.
- W2114885153 cites W1993689943 @default.
- W2114885153 cites W1996450190 @default.
- W2114885153 cites W2013845005 @default.
- W2114885153 cites W2031990419 @default.
- W2114885153 cites W2034316379 @default.
- W2114885153 cites W2034767282 @default.
- W2114885153 cites W2037883522 @default.
- W2114885153 cites W2038651535 @default.
- W2114885153 cites W2041810325 @default.
- W2114885153 cites W2044097410 @default.
- W2114885153 cites W2052723491 @default.
- W2114885153 cites W2068530940 @default.
- W2114885153 cites W2079029116 @default.
- W2114885153 cites W2085531572 @default.
- W2114885153 cites W2088518611 @default.
- W2114885153 cites W2090575626 @default.
- W2114885153 cites W2097233732 @default.
- W2114885153 cites W2098014201 @default.
- W2114885153 cites W2100837269 @default.
- W2114885153 cites W2123193725 @default.
- W2114885153 cites W2125811524 @default.
- W2114885153 cites W2125865118 @default.
- W2114885153 cites W2128741374 @default.
- W2114885153 cites W2134280546 @default.
- W2114885153 cites W2140733984 @default.
- W2114885153 cites W2166687321 @default.
- W2114885153 cites W2210708074 @default.
- W2114885153 cites W4293247451 @default.
- W2114885153 doi "https://doi.org/10.1371/journal.pone.0080066" @default.
- W2114885153 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3832651" @default.
- W2114885153 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24260337" @default.
- W2114885153 hasPublicationYear "2013" @default.
- W2114885153 type Work @default.
- W2114885153 sameAs 2114885153 @default.
- W2114885153 citedByCount "10" @default.
- W2114885153 countsByYear W21148851532015 @default.
- W2114885153 countsByYear W21148851532016 @default.
- W2114885153 countsByYear W21148851532017 @default.
- W2114885153 countsByYear W21148851532019 @default.
- W2114885153 countsByYear W21148851532020 @default.
- W2114885153 countsByYear W21148851532022 @default.
- W2114885153 crossrefType "journal-article" @default.
- W2114885153 hasAuthorship W2114885153A5001446297 @default.
- W2114885153 hasAuthorship W2114885153A5027818166 @default.
- W2114885153 hasAuthorship W2114885153A5061841750 @default.
- W2114885153 hasAuthorship W2114885153A5065085743 @default.
- W2114885153 hasAuthorship W2114885153A5065614485 @default.
- W2114885153 hasAuthorship W2114885153A5085760582 @default.
- W2114885153 hasAuthorship W2114885153A5089897001 @default.
- W2114885153 hasAuthorship W2114885153A5090479609 @default.
- W2114885153 hasBestOaLocation W21148851531 @default.
- W2114885153 hasConcept C102789151 @default.
- W2114885153 hasConcept C168197293 @default.
- W2114885153 hasConcept C181199279 @default.
- W2114885153 hasConcept C2776704645 @default.
- W2114885153 hasConcept C2777328355 @default.
- W2114885153 hasConcept C2778757898 @default.
- W2114885153 hasConcept C2779697368 @default.
- W2114885153 hasConcept C2780618852 @default.
- W2114885153 hasConcept C28212737 @default.
- W2114885153 hasConcept C55493867 @default.
- W2114885153 hasConcept C59822182 @default.
- W2114885153 hasConcept C64778439 @default.
- W2114885153 hasConcept C64915919 @default.
- W2114885153 hasConcept C86803240 @default.
- W2114885153 hasConcept C94412978 @default.
- W2114885153 hasConceptScore W2114885153C102789151 @default.
- W2114885153 hasConceptScore W2114885153C168197293 @default.
- W2114885153 hasConceptScore W2114885153C181199279 @default.
- W2114885153 hasConceptScore W2114885153C2776704645 @default.
- W2114885153 hasConceptScore W2114885153C2777328355 @default.