FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2114885512> ?p ?o ?g. }

• W2114885512 endingPage "588" @default.
• W2114885512 startingPage "583" @default.
• W2114885512 abstract "Diabodies are the recombinant bispecific antibodies (BsAbs), constructed from heterogeneous single-chain antibodies. Usually, diabodies have been prepared from bacterial periplasmic fraction using a co-expression vector (i.e. genes encoding two chains were tandemly located under the same promoter). Some diabodies, however, cannot be expressed as a soluble material owing to inclusion body formation, which limits the utilization of diabodies in various fields. Here we report an improved method for the construction of diabodies using a refolding system. As a model, a bispecific diabody binding to adenocarcinoma-associated antigen MUC1 and to CD3 on T cells was studied. One chain consisted of a VH specific for MUC1 linked to a VL specific for CD3 with a short polypeptide linker (GGGGS). The second was composed of a VL specific for MUC1 linked to a VH specific for CD3. The two hetero scFvs were independently obtained from intracellular insoluble fractions of Escherichia coli, purified, mixed stoichiometrically (at an equivalent molar ratio of 1:1) and refolded. The refolded two hetero scFv has a hetero-dimeric structure, with complete specificity for both target cells [i.e. MUC1 positive cells and CD3 positive lymphokine-activated killer cells with a T cell phenotype (T-LAK)]. Evaluation of the in vitro efficacy of T-LAK with the diabody by growth inhibition assay of cancer cells demonstrated maximum growth inhibition of cancer cells to reach approximately 98% at an effector:target ratio (E:T ratio) of 10, almost identical with that with anti-MUC1xanti-CD3 chemically synthesized BsAbs (c-BsAbs). This is the first report of the construction of a diabody using a refolding system." @default.
• W2114885512 created "2016-06-24" @default.
• W2114885512 creator A5000531538 @default.
• W2114885512 creator A5002372254 @default.
• W2114885512 creator A5006617820 @default.
• W2114885512 creator A5007072632 @default.
• W2114885512 creator A5010872554 @default.
• W2114885512 creator A5051654730 @default.
• W2114885512 creator A5054549197 @default.
• W2114885512 creator A5056688756 @default.
• W2114885512 creator A5060815670 @default.
• W2114885512 creator A5064820734 @default.
• W2114885512 creator A5070797459 @default.
• W2114885512 creator A5072979871 @default.
• W2114885512 creator A5089856009 @default.
• W2114885512 date "2000-08-01" @default.
• W2114885512 modified "2023-10-16" @default.
• W2114885512 title "Construction of a diabody (small recombinant bispecific antibody) using a refolding system" @default.
• W2114885512 cites W1976752775 @default.
• W2114885512 cites W1982501635 @default.
• W2114885512 cites W1995562726 @default.
• W2114885512 cites W1996054826 @default.
• W2114885512 cites W1996820981 @default.
• W2114885512 cites W2009179035 @default.
• W2114885512 cites W2022468667 @default.
• W2114885512 cites W2036217997 @default.
• W2114885512 cites W2036931150 @default.
• W2114885512 cites W2059410948 @default.
• W2114885512 cites W2081732271 @default.
• W2114885512 cites W2100837269 @default.
• W2114885512 cites W2108124504 @default.
• W2114885512 cites W2115820004 @default.
• W2114885512 cites W2126181518 @default.
• W2114885512 cites W2135344518 @default.
• W2114885512 cites W2138701221 @default.
• W2114885512 cites W2141212534 @default.
• W2114885512 cites W2144026567 @default.
• W2114885512 cites W2155018679 @default.
• W2114885512 cites W2162183933 @default.
• W2114885512 cites W2254120162 @default.
• W2114885512 cites W2482394274 @default.
• W2114885512 doi "https://doi.org/10.1093/protein/13.8.583" @default.
• W2114885512 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10964988" @default.
• W2114885512 hasPublicationYear "2000" @default.
• W2114885512 type Work @default.
• W2114885512 sameAs 2114885512 @default.
• W2114885512 citedByCount "30" @default.
• W2114885512 countsByYear W21148855122012 @default.
• W2114885512 countsByYear W21148855122013 @default.
• W2114885512 countsByYear W21148855122015 @default.
• W2114885512 countsByYear W21148855122017 @default.
• W2114885512 countsByYear W21148855122018 @default.
• W2114885512 countsByYear W21148855122020 @default.
• W2114885512 crossrefType "journal-article" @default.
• W2114885512 hasAuthorship W2114885512A5000531538 @default.
• W2114885512 hasAuthorship W2114885512A5002372254 @default.
• W2114885512 hasAuthorship W2114885512A5006617820 @default.
• W2114885512 hasAuthorship W2114885512A5007072632 @default.
• W2114885512 hasAuthorship W2114885512A5010872554 @default.
• W2114885512 hasAuthorship W2114885512A5051654730 @default.
• W2114885512 hasAuthorship W2114885512A5054549197 @default.
• W2114885512 hasAuthorship W2114885512A5056688756 @default.
• W2114885512 hasAuthorship W2114885512A5060815670 @default.
• W2114885512 hasAuthorship W2114885512A5064820734 @default.
• W2114885512 hasAuthorship W2114885512A5070797459 @default.
• W2114885512 hasAuthorship W2114885512A5072979871 @default.
• W2114885512 hasAuthorship W2114885512A5089856009 @default.
• W2114885512 hasBestOaLocation W21148855121 @default.
• W2114885512 hasConcept C104317684 @default.
• W2114885512 hasConcept C114684123 @default.
• W2114885512 hasConcept C147483822 @default.
• W2114885512 hasConcept C153911025 @default.
• W2114885512 hasConcept C159654299 @default.
• W2114885512 hasConcept C167672396 @default.
• W2114885512 hasConcept C179264091 @default.
• W2114885512 hasConcept C185592680 @default.
• W2114885512 hasConcept C201663137 @default.
• W2114885512 hasConcept C202751555 @default.
• W2114885512 hasConcept C203014093 @default.
• W2114885512 hasConcept C2777421810 @default.
• W2114885512 hasConcept C40767141 @default.
• W2114885512 hasConcept C542903549 @default.
• W2114885512 hasConcept C547475151 @default.
• W2114885512 hasConcept C55493867 @default.
• W2114885512 hasConcept C86803240 @default.
• W2114885512 hasConceptScore W2114885512C104317684 @default.
• W2114885512 hasConceptScore W2114885512C114684123 @default.
• W2114885512 hasConceptScore W2114885512C147483822 @default.
• W2114885512 hasConceptScore W2114885512C153911025 @default.
• W2114885512 hasConceptScore W2114885512C159654299 @default.
• W2114885512 hasConceptScore W2114885512C167672396 @default.
• W2114885512 hasConceptScore W2114885512C179264091 @default.
• W2114885512 hasConceptScore W2114885512C185592680 @default.
• W2114885512 hasConceptScore W2114885512C201663137 @default.
• W2114885512 hasConceptScore W2114885512C202751555 @default.
• W2114885512 hasConceptScore W2114885512C203014093 @default.
• W2114885512 hasConceptScore W2114885512C2777421810 @default.
• W2114885512 hasConceptScore W2114885512C40767141 @default.

• next