FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2114898983> ?p ?o ?g. }

• W2114898983 endingPage "3936" @default.
• W2114898983 startingPage "3923" @default.
• W2114898983 abstract "ABSTRACT A newly recognized Serratia species, termed South African Caenorhabditis briggsae isolate (SCBI), is both a mutualist of the nematode Caenorhabditis briggsae KT0001 and a pathogen of lepidopteran insects. Serratia sp. strain SCBI displays high proteolytic activity, and because secreted proteases are known virulence factors for many pathogens, the purpose of this study was to identify genes essential for extracellular protease activity in Serratia sp. strain SCBI and to determine what role proteases play in insect pathogenesis and cytotoxicity. A bank of 2,100 transposon mutants was generated, and six SCBI mutants with defective proteolytic activity were identified. These mutants were also defective in cytotoxicity. The mutants were found defective in genes encoding the following proteins: alkaline metalloprotease secretion protein AprE, a BglB family transcriptional antiterminator, an inosine/xanthosine triphosphatase, GidA, a methyl-accepting chemotaxis protein, and a PIN domain protein. Gene expression analysis on these six mutants showed significant downregulation in mRNA levels of several different types of predicted protease genes. In addition, transcriptome sequencing (RNA-seq) analysis provided insight into how inactivation of AprE, GidA, and a PIN domain protein influences motility and virulence, as well as protease activity. Using quantitative reverse transcription-PCR (qRT-PCR) to further characterize expression of predicted protease genes in wild-type Serratia sp. SCBI, the highest mRNA levels for the alkaline metalloprotease genes (termed prtA1 to prtA4 ) occurred following the death of an insect host, while two serine protease and two metalloprotease genes had their highest mRNA levels during active infection. Overall, these results indicate that proteolytic activity is essential for cytotoxicity in Serratia sp. SCBI and that its regulation appears to be highly complex." @default.
• W2114898983 created "2016-06-24" @default.
• W2114898983 creator A5073315946 @default.
• W2114898983 creator A5086075689 @default.
• W2114898983 date "2014-11-15" @default.
• W2114898983 modified "2023-10-11" @default.
• W2114898983 title "Molecular Characterization of Protease Activity in Serratia sp. Strain SCBI and Its Importance in Cytotoxicity and Virulence" @default.
• W2114898983 cites W1377731 @default.
• W2114898983 cites W1558653051 @default.
• W2114898983 cites W1568117350 @default.
• W2114898983 cites W1572897384 @default.
• W2114898983 cites W1708604775 @default.
• W2114898983 cites W1894220132 @default.
• W2114898983 cites W1910451772 @default.
• W2114898983 cites W1923118929 @default.
• W2114898983 cites W1975296288 @default.
• W2114898983 cites W1976309051 @default.
• W2114898983 cites W1984974565 @default.
• W2114898983 cites W1994875540 @default.
• W2114898983 cites W1996804832 @default.
• W2114898983 cites W1999596775 @default.
• W2114898983 cites W2002328195 @default.
• W2114898983 cites W2009017924 @default.
• W2114898983 cites W2011375465 @default.
• W2114898983 cites W2013511695 @default.
• W2114898983 cites W2014258776 @default.
• W2114898983 cites W2016549645 @default.
• W2114898983 cites W2019606456 @default.
• W2114898983 cites W2023091213 @default.
• W2114898983 cites W2024398218 @default.
• W2114898983 cites W2027229485 @default.
• W2114898983 cites W2030477444 @default.
• W2114898983 cites W2039997350 @default.
• W2114898983 cites W2057598612 @default.
• W2114898983 cites W2062130335 @default.
• W2114898983 cites W2062553509 @default.
• W2114898983 cites W2074619179 @default.
• W2114898983 cites W2084894372 @default.
• W2114898983 cites W2085106644 @default.
• W2114898983 cites W2090476579 @default.
• W2114898983 cites W2092579362 @default.
• W2114898983 cites W2093185569 @default.
• W2114898983 cites W2094708473 @default.
• W2114898983 cites W2097191787 @default.
• W2114898983 cites W2097899215 @default.
• W2114898983 cites W2098464894 @default.
• W2114898983 cites W2101075180 @default.
• W2114898983 cites W2102240998 @default.
• W2114898983 cites W2103848934 @default.
• W2114898983 cites W2111194455 @default.
• W2114898983 cites W2112822318 @default.
• W2114898983 cites W2114183422 @default.
• W2114898983 cites W2119583218 @default.
• W2114898983 cites W2120213729 @default.
• W2114898983 cites W2123704093 @default.
• W2114898983 cites W2134066586 @default.
• W2114898983 cites W2136317422 @default.
• W2114898983 cites W2145207673 @default.
• W2114898983 cites W2150254458 @default.
• W2114898983 cites W2150372522 @default.
• W2114898983 cites W2160084442 @default.
• W2114898983 cites W2160309830 @default.
• W2114898983 cites W2161733098 @default.
• W2114898983 cites W2165116172 @default.
• W2114898983 cites W2170446180 @default.
• W2114898983 cites W2291052550 @default.
• W2114898983 cites W2319901721 @default.
• W2114898983 cites W2344380565 @default.
• W2114898983 cites W4211069847 @default.
• W2114898983 doi "https://doi.org/10.1128/jb.01908-14" @default.
• W2114898983 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4248818" @default.
• W2114898983 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25182493" @default.
• W2114898983 hasPublicationYear "2014" @default.
• W2114898983 type Work @default.
• W2114898983 sameAs 2114898983 @default.
• W2114898983 citedByCount "21" @default.
• W2114898983 countsByYear W21148989832015 @default.
• W2114898983 countsByYear W21148989832016 @default.
• W2114898983 countsByYear W21148989832018 @default.
• W2114898983 countsByYear W21148989832019 @default.
• W2114898983 countsByYear W21148989832020 @default.
• W2114898983 countsByYear W21148989832021 @default.
• W2114898983 countsByYear W21148989832022 @default.
• W2114898983 countsByYear W21148989832023 @default.
• W2114898983 crossrefType "journal-article" @default.
• W2114898983 hasAuthorship W2114898983A5073315946 @default.
• W2114898983 hasAuthorship W2114898983A5086075689 @default.
• W2114898983 hasBestOaLocation W21148989831 @default.
• W2114898983 hasConcept C104317684 @default.
• W2114898983 hasConcept C143065580 @default.
• W2114898983 hasConcept C181199279 @default.
• W2114898983 hasConcept C182220744 @default.
• W2114898983 hasConcept C2776714187 @default.
• W2114898983 hasConcept C2777807008 @default.
• W2114898983 hasConcept C54355233 @default.
• W2114898983 hasConcept C55493867 @default.
• W2114898983 hasConcept C60987743 @default.
• W2114898983 hasConcept C86803240 @default.

• next