FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2115016737> ?p ?o ?g. }

• W2115016737 endingPage "13" @default.
• W2115016737 startingPage "1" @default.
• W2115016737 abstract "This work compares the removal of bovine aortic endothelial cell (BAEC) monolayers via 1) low-temperature liftoff from a “smart polymer,” plasma polymerized poly(N-isopropyl acrylamide) (ppNIPAM), 2) enzymatic digestion, and 3) mechanical dissociation from ppNIPAM surfaces. We examine the surfaces after cell removal by using X-ray photoelectron spectroscopy (XPS), time-of-flight secondary ion mass spectrometry (ToF-SIMS), immunostaining, and cell adhesion assay. Immunoassay results indicate that low-temperature liftoff nondestructively harvests the cell sheet and most of the underlying extracellular matrix (ECM), whereas enzymatic digestion and mechanical dissociation are damaging to both the cells and ECM. XPS results indicate that amide and alcohol groups attributed to proteins in the ECM are present on postliftoff surfaces. Principal component analysis (PCA) of ToF-SIMS data indicates that molecular ion fragments of amino acids are present on postliftoff surfaces. Finally, a cell adhesion assay seeding new cells on surfaces from which an initial layer of cells was removed via each of the three methods indicates that liftoff and mechanical dissociation leave behind surfaces that better promote cell adhesion. We conclude that the removal of BAEC cells via low-temperature liftoff from ppNIPAM-treated surfaces is less damaging to the ECM proteins remaining at the surface than the other methods. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005" @default.
• W2115016737 created "2016-06-24" @default.
• W2115016737 creator A5013411400 @default.
• W2115016737 creator A5028127818 @default.
• W2115016737 creator A5032777884 @default.
• W2115016737 creator A5034127767 @default.
• W2115016737 creator A5049441963 @default.
• W2115016737 date "2005-10-01" @default.
• W2115016737 modified "2023-10-01" @default.
• W2115016737 title "Cell sheet detachment affects the extracellular matrix: A surface science study comparing thermal liftoff, enzymatic, and mechanical methods" @default.
• W2115016737 cites W1963780254 @default.
• W2115016737 cites W1969907529 @default.
• W2115016737 cites W1971553231 @default.
• W2115016737 cites W1975471033 @default.
• W2115016737 cites W1980780439 @default.
• W2115016737 cites W1981211088 @default.
• W2115016737 cites W1984267955 @default.
• W2115016737 cites W1987718692 @default.
• W2115016737 cites W1997262547 @default.
• W2115016737 cites W1999369954 @default.
• W2115016737 cites W2008259524 @default.
• W2115016737 cites W2010365308 @default.
• W2115016737 cites W2013912007 @default.
• W2115016737 cites W2017480916 @default.
• W2115016737 cites W2017904896 @default.
• W2115016737 cites W2020134334 @default.
• W2115016737 cites W2027972137 @default.
• W2115016737 cites W2029231027 @default.
• W2115016737 cites W2038387332 @default.
• W2115016737 cites W2038691038 @default.
• W2115016737 cites W2040509978 @default.
• W2115016737 cites W2041058119 @default.
• W2115016737 cites W2046697805 @default.
• W2115016737 cites W2047710494 @default.
• W2115016737 cites W2055287838 @default.
• W2115016737 cites W2059231183 @default.
• W2115016737 cites W2063599661 @default.
• W2115016737 cites W2066119650 @default.
• W2115016737 cites W2066367871 @default.
• W2115016737 cites W2070839155 @default.
• W2115016737 cites W2073560699 @default.
• W2115016737 cites W2075705257 @default.
• W2115016737 cites W2079728694 @default.
• W2115016737 cites W2079765545 @default.
• W2115016737 cites W2080414132 @default.
• W2115016737 cites W2085596409 @default.
• W2115016737 cites W2085904428 @default.
• W2115016737 cites W2086525212 @default.
• W2115016737 cites W2086710884 @default.
• W2115016737 cites W2087967715 @default.
• W2115016737 cites W2089468765 @default.
• W2115016737 cites W2090814570 @default.
• W2115016737 cites W2091573294 @default.
• W2115016737 cites W2095290416 @default.
• W2115016737 cites W2098006861 @default.
• W2115016737 cites W2117676914 @default.
• W2115016737 cites W2123787315 @default.
• W2115016737 cites W2128644013 @default.
• W2115016737 cites W2140152493 @default.
• W2115016737 cites W2155370077 @default.
• W2115016737 doi "https://doi.org/10.1002/jbm.a.30297" @default.
• W2115016737 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16086418" @default.
• W2115016737 hasPublicationYear "2005" @default.
• W2115016737 type Work @default.
• W2115016737 sameAs 2115016737 @default.
• W2115016737 citedByCount "198" @default.
• W2115016737 countsByYear W21150167372012 @default.
• W2115016737 countsByYear W21150167372013 @default.
• W2115016737 countsByYear W21150167372014 @default.
• W2115016737 countsByYear W21150167372015 @default.
• W2115016737 countsByYear W21150167372016 @default.
• W2115016737 countsByYear W21150167372017 @default.
• W2115016737 countsByYear W21150167372018 @default.
• W2115016737 countsByYear W21150167372019 @default.
• W2115016737 countsByYear W21150167372020 @default.
• W2115016737 countsByYear W21150167372021 @default.
• W2115016737 countsByYear W21150167372022 @default.
• W2115016737 countsByYear W21150167372023 @default.
• W2115016737 crossrefType "journal-article" @default.
• W2115016737 hasAuthorship W2115016737A5013411400 @default.
• W2115016737 hasAuthorship W2115016737A5028127818 @default.
• W2115016737 hasAuthorship W2115016737A5032777884 @default.
• W2115016737 hasAuthorship W2115016737A5034127767 @default.
• W2115016737 hasAuthorship W2115016737A5049441963 @default.
• W2115016737 hasConcept C113196181 @default.
• W2115016737 hasConcept C12554922 @default.
• W2115016737 hasConcept C127413603 @default.
• W2115016737 hasConcept C159985019 @default.
• W2115016737 hasConcept C162356407 @default.
• W2115016737 hasConcept C175708663 @default.
• W2115016737 hasConcept C185592680 @default.
• W2115016737 hasConcept C189165786 @default.
• W2115016737 hasConcept C192562407 @default.
• W2115016737 hasConcept C42360764 @default.
• W2115016737 hasConcept C43617362 @default.
• W2115016737 hasConcept C55493867 @default.
• W2115016737 hasConcept C77671233 @default.
• W2115016737 hasConcept C84416704 @default.

• next