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• W2115083480 abstract "Abstract Aim: Endometrial carcinoma (EC) is a common gynecologic malignancy. EC has a favorable prognosis because it is usually diagnosed at an early stage. However, the recurrence rate is high and the prognosis is poor for high‐risk EC. Identification of new biomarkers for the prediction of high‐risk features will help to guide the treatment and improve the prognosis of patients with EC. Material and Methods: Differentially expressed proteins among high‐risk EC, low‐risk EC, and normal endometrial tissues were determined by two‐dimensional gel electrophoresis (2‐DE) and a liquid chromatography electrospray ionization tandem mass spectrometry (LC–ESI–MS/MS) proteomics approach. Then, the candidate proteins were examined by immunohistochemical analysis. Results: Thirteen protein spots were differentially expressed between the high‐ and low‐risk groups, and 25 protein spots were differentially expressed between the high‐risk and normal endometrium groups. Twenty‐two proteins were identified by MS analysis. PKM2 and HSPA5 were elevated in the high‐risk EC tissues compared with both the low‐risk EC and normal endometrial tissues. The elevated expression of PKM2 and HSPA5 in high‐risk EC tissue was confirmed by immunohistochemical analysis. Discussion: PKM2 and HSPA5 may play an important role in the progression of EC. These two proteins are potential biomarkers to better predict high‐risk EC and thereby guide clinical therapy." @default.
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• W2115083480 date "2012-08-13" @default.
• W2115083480 modified "2023-10-16" @default.
• W2115083480 title "Proteomic identification of PKM2 and HSPA5 as potential biomarkers for predicting high-risk endometrial carcinoma" @default.
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• W2115083480 doi "https://doi.org/10.1111/j.1447-0756.2012.01970.x" @default.
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