Matches in SemOpenAlex for { <https://semopenalex.org/work/W2115091844> ?p ?o ?g. }
- W2115091844 endingPage "113" @default.
- W2115091844 startingPage "105" @default.
- W2115091844 abstract "Bovine embryos cultured in serum-containing media abnormally accumulate lipid droplets, compared to their in vivo counterparts. The objective of this study was to investigate the effect of different culture systems on the mRNA expression and on the quantification and localisation of adipocyte differentiation-related protein (ADRP), a protein associated with lipid accumulation in bovine blastocysts. Two experiments were independently performed for ADRP mRNA expression analysis. In experiment A, blastocysts were produced in modified synthetic oviduct fluid (mSOF) + 10% foetal calf serum (FCS), in coculture (bovine oviduct epithelial cells, Boec) and in ewe oviducts, whereas in experiment B, they were produced in mSOF + 10 μM docosahexaenoic acid (DHA) and in vivo. Control groups were also performed. ADRP mRNA expression was downregulated in the Boec, ewe oviduct and in vivo groups compared to the 10% FCS or DHA groups, respectively. Moreover, the expression of this protein was downregulated in the Boec group compared to the control group (P < 0.05). A third experiment (experiment C) was performed to quantify and localise ADRP protein. Boec, in vivo and control groups were tested. After immunofluorescence staining followed by confocal microscopy analysis, embryonic ADRP was clearly localised around lipid droplets, indicating that ADRP is also a lipid droplet coat protein in bovine embryos. In conclusion, our results demonstrate that bovine embryos at the blastocyst stage expressed ADRP mRNA and protein, and that the embryonic culture system modified this expression." @default.
- W2115091844 created "2016-06-24" @default.
- W2115091844 creator A5010231796 @default.
- W2115091844 creator A5021004568 @default.
- W2115091844 creator A5021437775 @default.
- W2115091844 creator A5024981277 @default.
- W2115091844 creator A5026512129 @default.
- W2115091844 creator A5059013869 @default.
- W2115091844 creator A5066485177 @default.
- W2115091844 creator A5067588298 @default.
- W2115091844 creator A5091740543 @default.
- W2115091844 date "2014-03-01" @default.
- W2115091844 modified "2023-09-25" @default.
- W2115091844 title "Effect of different culture systems on adipocyte differentiation-related protein (ADRP) in bovine embryos" @default.
- W2115091844 cites W1965039157 @default.
- W2115091844 cites W1966202390 @default.
- W2115091844 cites W1971843304 @default.
- W2115091844 cites W1975732952 @default.
- W2115091844 cites W1976339009 @default.
- W2115091844 cites W1987353211 @default.
- W2115091844 cites W1988600076 @default.
- W2115091844 cites W1989727348 @default.
- W2115091844 cites W1989739763 @default.
- W2115091844 cites W1995122254 @default.
- W2115091844 cites W1996524332 @default.
- W2115091844 cites W1999431599 @default.
- W2115091844 cites W1999438839 @default.
- W2115091844 cites W1999861330 @default.
- W2115091844 cites W2003936232 @default.
- W2115091844 cites W2008037659 @default.
- W2115091844 cites W2010082323 @default.
- W2115091844 cites W2010866880 @default.
- W2115091844 cites W2014059216 @default.
- W2115091844 cites W2022037630 @default.
- W2115091844 cites W2023272679 @default.
- W2115091844 cites W2024157970 @default.
- W2115091844 cites W2026577112 @default.
- W2115091844 cites W2033823700 @default.
- W2115091844 cites W2036925535 @default.
- W2115091844 cites W2039607766 @default.
- W2115091844 cites W2042114928 @default.
- W2115091844 cites W2042370696 @default.
- W2115091844 cites W2043237666 @default.
- W2115091844 cites W2044491218 @default.
- W2115091844 cites W2053948748 @default.
- W2115091844 cites W2066050953 @default.
- W2115091844 cites W2067490842 @default.
- W2115091844 cites W2069583796 @default.
- W2115091844 cites W2091954672 @default.
- W2115091844 cites W2097521595 @default.
- W2115091844 cites W2100149389 @default.
- W2115091844 cites W2100834464 @default.
- W2115091844 cites W2119598306 @default.
- W2115091844 cites W2121011454 @default.
- W2115091844 cites W2131290128 @default.
- W2115091844 cites W2131765514 @default.
- W2115091844 cites W2135016167 @default.
- W2115091844 cites W2137919453 @default.
- W2115091844 cites W2138377765 @default.
- W2115091844 cites W2139658711 @default.
- W2115091844 cites W2142187706 @default.
- W2115091844 cites W2143699233 @default.
- W2115091844 cites W2144475476 @default.
- W2115091844 cites W2145466720 @default.
- W2115091844 cites W2170717683 @default.
- W2115091844 cites W4230011011 @default.
- W2115091844 cites W4252926782 @default.
- W2115091844 cites W43060411 @default.
- W2115091844 doi "https://doi.org/10.1016/j.anireprosci.2014.01.010" @default.
- W2115091844 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24560670" @default.
- W2115091844 hasPublicationYear "2014" @default.
- W2115091844 type Work @default.
- W2115091844 sameAs 2115091844 @default.
- W2115091844 citedByCount "7" @default.
- W2115091844 countsByYear W21150918442015 @default.
- W2115091844 countsByYear W21150918442016 @default.
- W2115091844 countsByYear W21150918442017 @default.
- W2115091844 countsByYear W21150918442018 @default.
- W2115091844 countsByYear W21150918442019 @default.
- W2115091844 countsByYear W21150918442022 @default.
- W2115091844 crossrefType "journal-article" @default.
- W2115091844 hasAuthorship W2115091844A5010231796 @default.
- W2115091844 hasAuthorship W2115091844A5021004568 @default.
- W2115091844 hasAuthorship W2115091844A5021437775 @default.
- W2115091844 hasAuthorship W2115091844A5024981277 @default.
- W2115091844 hasAuthorship W2115091844A5026512129 @default.
- W2115091844 hasAuthorship W2115091844A5059013869 @default.
- W2115091844 hasAuthorship W2115091844A5066485177 @default.
- W2115091844 hasAuthorship W2115091844A5067588298 @default.
- W2115091844 hasAuthorship W2115091844A5091740543 @default.
- W2115091844 hasConcept C104317684 @default.
- W2115091844 hasConcept C105580179 @default.
- W2115091844 hasConcept C134018914 @default.
- W2115091844 hasConcept C153911025 @default.
- W2115091844 hasConcept C16685009 @default.
- W2115091844 hasConcept C185592680 @default.
- W2115091844 hasConcept C196843134 @default.
- W2115091844 hasConcept C207001950 @default.