Matches in SemOpenAlex for { <https://semopenalex.org/work/W2115135270> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2115135270 endingPage "402" @default.
- W2115135270 startingPage "395" @default.
- W2115135270 abstract "Asp187 and Gln190 were predicted as conserved and closely located at the Na(+) binding site in a topology and homology model structure of Na(+)/proline symporter (PutP) of Escherichia coli. The replacement of Asp187 with Ala or Leu did not affect proline transport activity; whereas, change to Gln abolished the active transport. The binding affinity for Na(+) or proline of these mutants was similar to that of wild-type (WT) PutP. This result indicates Asp187 to be responsible for active transport of proline without affecting the binding. Replacement of Gln190 with Ala, Asn, Asp, Leu and Glu had no effect on transport or binding, suggesting that it may not have a role in the transport. However, in the negative D187Q mutant, a second mutation, of Gln190 to Glu or Leu, restored 46 or 7% of the transport activity of WT, respectively, while mutation to Ala, Asn or Asp had no effect. Thus, side chain at position 190 has a crucial role in suppressing the functional defect of the D187Q mutant. We conclude that Asp187 is responsible for transport activity instead of coupling-ion binding by constituting the translocation pathway of the ion and Gln190 provides a suppressing mutation site to regain PutP functional activity." @default.
- W2115135270 created "2016-06-24" @default.
- W2115135270 creator A5014448041 @default.
- W2115135270 creator A5021891857 @default.
- W2115135270 creator A5024291515 @default.
- W2115135270 creator A5044805307 @default.
- W2115135270 date "2011-05-17" @default.
- W2115135270 modified "2023-10-18" @default.
- W2115135270 title "Role of Asp187 and Gln190 in the Na+/proline symporter (PutP) of Escherichia coli" @default.
- W2115135270 cites W1969419295 @default.
- W2115135270 cites W1977078160 @default.
- W2115135270 cites W1979479735 @default.
- W2115135270 cites W1986143860 @default.
- W2115135270 cites W1997529081 @default.
- W2115135270 cites W2014594199 @default.
- W2115135270 cites W2015642465 @default.
- W2115135270 cites W2020227804 @default.
- W2115135270 cites W2027212364 @default.
- W2115135270 cites W2033431317 @default.
- W2115135270 cites W2036599186 @default.
- W2115135270 cites W2043755841 @default.
- W2115135270 cites W2048468042 @default.
- W2115135270 cites W2049519918 @default.
- W2115135270 cites W2069817389 @default.
- W2115135270 cites W2070804112 @default.
- W2115135270 cites W2083882357 @default.
- W2115135270 cites W2084263041 @default.
- W2115135270 cites W2091590087 @default.
- W2115135270 cites W2099384461 @default.
- W2115135270 cites W2102131116 @default.
- W2115135270 cites W2104622728 @default.
- W2115135270 cites W2108161822 @default.
- W2115135270 cites W2124426317 @default.
- W2115135270 cites W2134296916 @default.
- W2115135270 cites W2145268834 @default.
- W2115135270 cites W2171043072 @default.
- W2115135270 cites W2187251803 @default.
- W2115135270 doi "https://doi.org/10.1093/jb/mvr062" @default.
- W2115135270 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21586535" @default.
- W2115135270 hasPublicationYear "2011" @default.
- W2115135270 type Work @default.
- W2115135270 sameAs 2115135270 @default.
- W2115135270 citedByCount "3" @default.
- W2115135270 countsByYear W21151352702013 @default.
- W2115135270 countsByYear W21151352702016 @default.
- W2115135270 countsByYear W21151352702020 @default.
- W2115135270 crossrefType "journal-article" @default.
- W2115135270 hasAuthorship W2115135270A5014448041 @default.
- W2115135270 hasAuthorship W2115135270A5021891857 @default.
- W2115135270 hasAuthorship W2115135270A5024291515 @default.
- W2115135270 hasAuthorship W2115135270A5044805307 @default.
- W2115135270 hasConcept C104317684 @default.
- W2115135270 hasConcept C107824862 @default.
- W2115135270 hasConcept C120405084 @default.
- W2115135270 hasConcept C143065580 @default.
- W2115135270 hasConcept C149011108 @default.
- W2115135270 hasConcept C153911025 @default.
- W2115135270 hasConcept C185592680 @default.
- W2115135270 hasConcept C207583985 @default.
- W2115135270 hasConcept C2779815552 @default.
- W2115135270 hasConcept C515207424 @default.
- W2115135270 hasConcept C547475151 @default.
- W2115135270 hasConcept C55493867 @default.
- W2115135270 hasConcept C86803240 @default.
- W2115135270 hasConceptScore W2115135270C104317684 @default.
- W2115135270 hasConceptScore W2115135270C107824862 @default.
- W2115135270 hasConceptScore W2115135270C120405084 @default.
- W2115135270 hasConceptScore W2115135270C143065580 @default.
- W2115135270 hasConceptScore W2115135270C149011108 @default.
- W2115135270 hasConceptScore W2115135270C153911025 @default.
- W2115135270 hasConceptScore W2115135270C185592680 @default.
- W2115135270 hasConceptScore W2115135270C207583985 @default.
- W2115135270 hasConceptScore W2115135270C2779815552 @default.
- W2115135270 hasConceptScore W2115135270C515207424 @default.
- W2115135270 hasConceptScore W2115135270C547475151 @default.
- W2115135270 hasConceptScore W2115135270C55493867 @default.
- W2115135270 hasConceptScore W2115135270C86803240 @default.
- W2115135270 hasIssue "4" @default.
- W2115135270 hasLocation W21151352701 @default.
- W2115135270 hasLocation W21151352702 @default.
- W2115135270 hasOpenAccess W2115135270 @default.
- W2115135270 hasPrimaryLocation W21151352701 @default.
- W2115135270 hasRelatedWork W1563006911 @default.
- W2115135270 hasRelatedWork W1971690527 @default.
- W2115135270 hasRelatedWork W1981142562 @default.
- W2115135270 hasRelatedWork W2082658611 @default.
- W2115135270 hasRelatedWork W2086527094 @default.
- W2115135270 hasRelatedWork W2160541634 @default.
- W2115135270 hasRelatedWork W2341645127 @default.
- W2115135270 hasRelatedWork W2378349133 @default.
- W2115135270 hasRelatedWork W2382939692 @default.
- W2115135270 hasRelatedWork W86974628 @default.
- W2115135270 hasVolume "150" @default.
- W2115135270 isParatext "false" @default.
- W2115135270 isRetracted "false" @default.
- W2115135270 magId "2115135270" @default.
- W2115135270 workType "article" @default.