FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2115148906> ?p ?o ?g. }

• W2115148906 endingPage "66" @default.
• W2115148906 startingPage "41" @default.
• W2115148906 abstract "SUMMARY Clostridium perfringens type-A α-toxin (phospholipase C) was purified on a preparative scale by a simple, rapid, two-stage procedure involving precipitation of culture-supernatant fluids with ammonium sulphate at 35 to 50% saturation, followed by isoelectric focusing in a pH 4.6 gradient. Milligram yields of highly purified α-toxin with 10 to 15% recovery of activity were obtained in single fractions. Two forms of α-toxin were identified by electrofocusing. The major peak of activity possessed a pI of 5.49±0.06(α A) and the minor, a pI of 5.25(α B). The former was free from detectable collagenase (pI 4.54), hyaluronidase (pI 4.73), θ-toxin (pI 6.56) and neuraminidase. It gave a single precipitin arc on immunoelectrophoresis, but analysis by SDS polyacrylamide disk-gel electrophoresis at high protein-loading revealed a major protein component of molecular weight 53,800 and two minor protein bands. Atomic emission spectro-scopy did not detect the presence of zinc in such preparations. The latter showed a single line of identity with α A in Ouchterlony gel diffusion tests and contained a protein component with the same molecular weight as that of α A. Fractions α A and α B both possessed hot-cold haemolytic, phospholipase-C, and lethal activities. Both hydrolysed lecithin and sphingomyelin. Electro-focusing of α-toxin in the presence of 6m urea resulted in the detection of only one component, α urea, with a pI identical to α A. It also possessed all three biological activities of α-toxin. Removal of the urea and refocusing was accompanied by the reappearance of α B. The occurrence and formation of α B could not be interpreted in terms of artefactual causes of multiple forms of proteins identified by isoelectric focusing. These studies provided evidence favouring the suggestion that α A and α B could be related as conformers, although aggregation or polymerisation could not be entirely excluded as possible alternative explanations." @default.
• W2115148906 created "2016-06-24" @default.
• W2115148906 creator A5019885999 @default.
• W2115148906 creator A5045913029 @default.
• W2115148906 date "1974-02-01" @default.
• W2115148906 modified "2023-09-25" @default.
• W2115148906 title "Properties of Clostridium Perfringens (Welch II) Type-A -Toxin (Phospholipase c) Purified By Electrofocusing" @default.
• W2115148906 cites W107865001 @default.
• W2115148906 cites W12014461 @default.
• W2115148906 cites W125833218 @default.
• W2115148906 cites W1488741230 @default.
• W2115148906 cites W1500626409 @default.
• W2115148906 cites W1508232735 @default.
• W2115148906 cites W1547694196 @default.
• W2115148906 cites W1587829775 @default.
• W2115148906 cites W1599375090 @default.
• W2115148906 cites W1761446686 @default.
• W2115148906 cites W1775749144 @default.
• W2115148906 cites W1844639619 @default.
• W2115148906 cites W1908408342 @default.
• W2115148906 cites W1968085537 @default.
• W2115148906 cites W198066573 @default.
• W2115148906 cites W1984540934 @default.
• W2115148906 cites W1988862188 @default.
• W2115148906 cites W1992613388 @default.
• W2115148906 cites W2030639097 @default.
• W2115148906 cites W2031924640 @default.
• W2115148906 cites W2048388421 @default.
• W2115148906 cites W2065627978 @default.
• W2115148906 cites W2066505507 @default.
• W2115148906 cites W2078436492 @default.
• W2115148906 cites W2080969503 @default.
• W2115148906 cites W2080985884 @default.
• W2115148906 cites W2083753610 @default.
• W2115148906 cites W2110517796 @default.
• W2115148906 cites W2115097414 @default.
• W2115148906 cites W2134399310 @default.
• W2115148906 cites W2149460441 @default.
• W2115148906 cites W2163721641 @default.
• W2115148906 cites W2172149134 @default.
• W2115148906 cites W2246021845 @default.
• W2115148906 cites W2325833922 @default.
• W2115148906 cites W2332395644 @default.
• W2115148906 cites W2332830582 @default.
• W2115148906 cites W2338865239 @default.
• W2115148906 cites W2404077416 @default.
• W2115148906 cites W2408698956 @default.
• W2115148906 cites W2409238342 @default.
• W2115148906 cites W2410379288 @default.
• W2115148906 cites W2410927170 @default.
• W2115148906 cites W2414375500 @default.
• W2115148906 cites W2968256092 @default.
• W2115148906 cites W345943304 @default.
• W2115148906 cites W640737932 @default.
• W2115148906 cites W66687617 @default.
• W2115148906 cites W91792183 @default.
• W2115148906 doi "https://doi.org/10.1099/00222615-7-1-41" @default.
• W2115148906 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4369079" @default.
• W2115148906 hasPublicationYear "1974" @default.
• W2115148906 type Work @default.
• W2115148906 sameAs 2115148906 @default.
• W2115148906 citedByCount "47" @default.
• W2115148906 countsByYear W21151489062014 @default.
• W2115148906 crossrefType "journal-article" @default.
• W2115148906 hasAuthorship W2115148906A5019885999 @default.
• W2115148906 hasAuthorship W2115148906A5045913029 @default.
• W2115148906 hasBestOaLocation W21151489061 @default.
• W2115148906 hasConcept C101660505 @default.
• W2115148906 hasConcept C181199279 @default.
• W2115148906 hasConcept C185592680 @default.
• W2115148906 hasConcept C2777367657 @default.
• W2115148906 hasConcept C2778597717 @default.
• W2115148906 hasConcept C2779116991 @default.
• W2115148906 hasConcept C2779155592 @default.
• W2115148906 hasConcept C2780365088 @default.
• W2115148906 hasConcept C43617362 @default.
• W2115148906 hasConcept C50476208 @default.
• W2115148906 hasConcept C523546767 @default.
• W2115148906 hasConcept C54355233 @default.
• W2115148906 hasConcept C55493867 @default.
• W2115148906 hasConcept C86803240 @default.
• W2115148906 hasConcept C96294017 @default.
• W2115148906 hasConceptScore W2115148906C101660505 @default.
• W2115148906 hasConceptScore W2115148906C181199279 @default.
• W2115148906 hasConceptScore W2115148906C185592680 @default.
• W2115148906 hasConceptScore W2115148906C2777367657 @default.
• W2115148906 hasConceptScore W2115148906C2778597717 @default.
• W2115148906 hasConceptScore W2115148906C2779116991 @default.
• W2115148906 hasConceptScore W2115148906C2779155592 @default.
• W2115148906 hasConceptScore W2115148906C2780365088 @default.
• W2115148906 hasConceptScore W2115148906C43617362 @default.
• W2115148906 hasConceptScore W2115148906C50476208 @default.
• W2115148906 hasConceptScore W2115148906C523546767 @default.
• W2115148906 hasConceptScore W2115148906C54355233 @default.
• W2115148906 hasConceptScore W2115148906C55493867 @default.
• W2115148906 hasConceptScore W2115148906C86803240 @default.
• W2115148906 hasConceptScore W2115148906C96294017 @default.
• W2115148906 hasIssue "1" @default.

• next