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• W2115156687 abstract "The aim of the present work was to genetically transform Scots pine (Pinus sylvestris L.) and Norway spruce (Picea abies (L.) Karst.) pollen in order to study the possibilities of obtaining transgenic progenies. The effects of different pollen origins and regulatory sequences on the β-glucuronidase (GUS) expression in pollen were significant. The highest GUS expression in pine (55 ± 7.7% of germinated pollen grains) was achieved with sunflower (Helianthus annuus) polyubiquitin promoter and in spruce (44 ± 12% of germinated pollen grains), by using an abscisic acid inducible promoter of wheat (Triticum aestivum) EM gene. Our results indicate that high levels of transient foreign gene expression can be achieved in pine and spruce pollen by using proper regulatory sequences for the target material and its physiological stage. Bombarded pollen was used for controlled pollinations in the greenhouse, after which 21% of the pine cones and 56% of the spruce cones developed normally. Resume : Ce travail visait ‡ transformer gOnOtiquement le pollen de pin sylvestre (Pinus sylvestris L.) et diOpicea commun (Picea abies (L.) Karst.) afin diOvaluer les possibilitOs diobtenir des descendances transgOniques. Liorigine du pollen et le type de sOquence rOgulatrice ont eu des effets significatifs sur liexpression de la β-glucuronidase (GUS). Chez le pin, liexpression la plus OlevOe de la β-glucuronidase (55 ± 7,7% des grains de pollen germOs) a OtO obtenue avec le promoteur de la polyubiquitine du tournesol. Chez liOpinette, la plus forte expression (44 ± 12% des grains de pollen germOs) fut obtenue en utilisant le promoteur inductible par liacide abscissique du gne EM du blO. Ces rOsultats indiquent que des niveaux OlevOs diexpression transitoire de gnes Otrangers peuvent Œtre obtenus chez le pollen de pin ou diOpinette en utilisant les sOquences rOgulatrices appropriOes en fonction du type de matOriel ‡ transformer et de son stade physiologique. Le pollen bombardO fut utilisO lors de pollinisations dirigOes en serre, oo 21% des cUnes de pin et 56% des cUnes diOpinette se sont dOveloppOs normalement. (Traduit par la ROdaction)" @default.
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• W2115156687 date "1997-01-01" @default.
• W2115156687 modified "2023-09-24" @default.
• W2115156687 title "Gene transfer by particle bombardment to Norway spruce and Scots pine pollen" @default.
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• W2115156687 doi "https://doi.org/10.1139/cjfr-27-6-928" @default.
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