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• W2115172015 abstract "Inductively coupled plasma dynamic reaction cell-quadrupole mass spectrometry (ICP-DRC-qMS) coupled on-line with anion exchange chromatography (AEC) has been developed for the quantification of selenium-tagged proteins in human plasma. Methane was employed as a reaction gas in the dynamic reaction cell to achieve the determination of 80Se free of spectroscopic interference from 40Ar2+ and 79BrH+. Five selenium species including selenoprotein P (SelP), glutathione peroxidase (GPx), selenoalbumin (SeAlb), and two unknown selenospecies (U1 and U2) in a pooled plasma sample from five healthy people were separated using AEC, and the distribution of selenium in SelP, GPx, SeAlb, U1 and U2 (about 45.5%, 19.1%, 15.1%, 2.9% and 8.1%) was determined by ICP-DRC-qMS using species-unspecific isotope dilution (80Se/77Se). Based on the detection limit (DL) of selenium (0.54 ng mL−1), we estimated that the DLs for SelP and GPx were 0.59 pmol mL−1 and 1.7 pmol mL−1, respectively. Through the stoichiometry of the selenium atom in the selenium-tagged proteins, SelP (2.7 ± 0.1 μg mL−1) and GPx (5.4 ± 0.2 μg mL−1) were successfully quantified." @default.
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• W2115172015 date "2008-01-01" @default.
• W2115172015 modified "2023-09-25" @default.
• W2115172015 title "Quantification of selenium-tagged proteins in human plasma using species-unspecific isotope dilution ICP-DRC-qMS coupled on-line with anion exchange chromatography" @default.
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• W2115172015 doi "https://doi.org/10.1039/b804935a" @default.
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