FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2115255364> ?p ?o ?g. }

• W2115255364 endingPage "16660" @default.
• W2115255364 startingPage "16655" @default.
• W2115255364 abstract "Herpes simplex virus type 2 (HSV-2) was the major cause of genital herpes in humans. The HSV-2 glycoprotein D (gD2) had been proved to be a potentially effective vaccine for treatment of genital herpes. The present study was to develop a two step culture to express the recombinant gD2 protein using the immobilized Spodoptera frugiperda (Sf9) cells. The first step, Sf9 cells were cultured and harvested in eutrophic medium containing 10% fetal bovine serum. The second step, Sf9 cells were immobilized using silk fibroin hydrogel and cultured in a stirred-tank bioreactor after infection by recombinant baculovirus expressing the full length gD2 gene. The data shows that the maximum yield of recombinant gD2 protein reached the concentration of 135 mg/L. The results reveal that the immobilized cells and two step culture could extend the expression period and significantly raise the production of the recombinant protein. Key words : Spodoptera frugiperda cells, baculovirus, immobilization, glycoprotein D, silk fibroin hydrogel." @default.
• W2115255364 created "2016-06-24" @default.
• W2115255364 creator A5003841581 @default.
• W2115255364 creator A5012273973 @default.
• W2115255364 creator A5062371912 @default.
• W2115255364 creator A5077317273 @default.
• W2115255364 creator A5083566143 @default.
• W2115255364 creator A5090532703 @default.
• W2115255364 date "2012-12-18" @default.
• W2115255364 modified "2023-09-29" @default.
• W2115255364 title "Two step culture for production of recombinant herpes simplex virus type 2 glycoprotein D in immobilized Spodoptera frugiperda cells" @default.
• W2115255364 cites W1965904253 @default.
• W2115255364 cites W1975514230 @default.
• W2115255364 cites W2007281760 @default.
• W2115255364 cites W2008486572 @default.
• W2115255364 cites W2019849247 @default.
• W2115255364 cites W2031344083 @default.
• W2115255364 cites W2034589644 @default.
• W2115255364 cites W2065866803 @default.
• W2115255364 cites W2139251542 @default.
• W2115255364 cites W2168284111 @default.
• W2115255364 cites W2183266028 @default.
• W2115255364 doi "https://doi.org/10.5897/ajb11.3356" @default.
• W2115255364 hasPublicationYear "2012" @default.
• W2115255364 type Work @default.
• W2115255364 sameAs 2115255364 @default.
• W2115255364 citedByCount "1" @default.
• W2115255364 countsByYear W21152553642020 @default.
• W2115255364 crossrefType "journal-article" @default.
• W2115255364 hasAuthorship W2115255364A5003841581 @default.
• W2115255364 hasAuthorship W2115255364A5012273973 @default.
• W2115255364 hasAuthorship W2115255364A5062371912 @default.
• W2115255364 hasAuthorship W2115255364A5077317273 @default.
• W2115255364 hasAuthorship W2115255364A5083566143 @default.
• W2115255364 hasAuthorship W2115255364A5090532703 @default.
• W2115255364 hasConcept C103878061 @default.
• W2115255364 hasConcept C104317684 @default.
• W2115255364 hasConcept C108625454 @default.
• W2115255364 hasConcept C122302759 @default.
• W2115255364 hasConcept C153911025 @default.
• W2115255364 hasConcept C159047783 @default.
• W2115255364 hasConcept C159985019 @default.
• W2115255364 hasConcept C185592680 @default.
• W2115255364 hasConcept C192562407 @default.
• W2115255364 hasConcept C2522874641 @default.
• W2115255364 hasConcept C2777773057 @default.
• W2115255364 hasConcept C2780431358 @default.
• W2115255364 hasConcept C2781196997 @default.
• W2115255364 hasConcept C39060996 @default.
• W2115255364 hasConcept C40767141 @default.
• W2115255364 hasConcept C54355233 @default.
• W2115255364 hasConcept C55493867 @default.
• W2115255364 hasConcept C81885089 @default.
• W2115255364 hasConcept C86803240 @default.
• W2115255364 hasConcept C89423630 @default.
• W2115255364 hasConceptScore W2115255364C103878061 @default.
• W2115255364 hasConceptScore W2115255364C104317684 @default.
• W2115255364 hasConceptScore W2115255364C108625454 @default.
• W2115255364 hasConceptScore W2115255364C122302759 @default.
• W2115255364 hasConceptScore W2115255364C153911025 @default.
• W2115255364 hasConceptScore W2115255364C159047783 @default.
• W2115255364 hasConceptScore W2115255364C159985019 @default.
• W2115255364 hasConceptScore W2115255364C185592680 @default.
• W2115255364 hasConceptScore W2115255364C192562407 @default.
• W2115255364 hasConceptScore W2115255364C2522874641 @default.
• W2115255364 hasConceptScore W2115255364C2777773057 @default.
• W2115255364 hasConceptScore W2115255364C2780431358 @default.
• W2115255364 hasConceptScore W2115255364C2781196997 @default.
• W2115255364 hasConceptScore W2115255364C39060996 @default.
• W2115255364 hasConceptScore W2115255364C40767141 @default.
• W2115255364 hasConceptScore W2115255364C54355233 @default.
• W2115255364 hasConceptScore W2115255364C55493867 @default.
• W2115255364 hasConceptScore W2115255364C81885089 @default.
• W2115255364 hasConceptScore W2115255364C86803240 @default.
• W2115255364 hasConceptScore W2115255364C89423630 @default.
• W2115255364 hasIssue "101" @default.
• W2115255364 hasLocation W21152553641 @default.
• W2115255364 hasOpenAccess W2115255364 @default.
• W2115255364 hasPrimaryLocation W21152553641 @default.
• W2115255364 hasRelatedWork W150805854 @default.
• W2115255364 hasRelatedWork W1974554319 @default.
• W2115255364 hasRelatedWork W1975352290 @default.
• W2115255364 hasRelatedWork W1986457809 @default.
• W2115255364 hasRelatedWork W2009418337 @default.
• W2115255364 hasRelatedWork W2023325679 @default.
• W2115255364 hasRelatedWork W2027117938 @default.
• W2115255364 hasRelatedWork W2056432280 @default.
• W2115255364 hasRelatedWork W2060613452 @default.
• W2115255364 hasRelatedWork W2065207168 @default.
• W2115255364 hasRelatedWork W2088143529 @default.
• W2115255364 hasRelatedWork W2360816808 @default.
• W2115255364 hasRelatedWork W2379884376 @default.
• W2115255364 hasRelatedWork W2383227538 @default.
• W2115255364 hasRelatedWork W2406121823 @default.
• W2115255364 hasRelatedWork W2410853672 @default.
• W2115255364 hasRelatedWork W2895089265 @default.
• W2115255364 hasRelatedWork W1993132601 @default.
• W2115255364 hasRelatedWork W2168088607 @default.

• next