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• W2115274299 startingPage "277" @default.
• W2115274299 abstract "Many aquatic species, such as teleosts, release into the water and detect multiple bioactive substances to assist in schooling, migration, alarm reactions, and to stimulate behavioral and physiological responses during reproduction and in parent-offspring interactions. Understanding the complex relationship between hormones, behavior and their function in communication requires the simultaneous examination of multiple circulating hormones. However, repeated blood sampling within a short time period is not possible in smaller animals without impacting the very behaviors under investigation. The non-invasive technique of collecting and measuring hormone values in holding water using either radioimmunoassay (RIA) or enzyme immunoassay (EIA) is becoming widely used in teleost research. Commercial assay kits in particular enable rapid and reliable data generation, yet their assay buffers are often specific and potentially incompatible with each other, which can hinder measuring multiple hormones from the same sample. We present here the validation and application of a nested elution technique we developed that allows for repeated sampling of multiple reproductive hormones - testosterone (T), 17beta-estradiol (E2), progesterone (P), prostaglandin F(2 alpha) (PGF) and 11-ketotestosterone (11KT) - from individual samples of animal holding water by using commercial EIA systems. Our results show that when using appropriate controls to account for possible technical and biological confounds, this technique provides a powerful new tool for research in aquatic endocrinology and physiology." @default.
• W2115274299 created "2016-06-24" @default.
• W2115274299 creator A5056486525 @default.
• W2115274299 creator A5068961586 @default.
• W2115274299 creator A5071767868 @default.
• W2115274299 date "2010-01-01" @default.
• W2115274299 modified "2023-09-26" @default.
• W2115274299 title "Measuring multiple hormones from a single water sample using enzyme immunoassays" @default.
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• W2115274299 doi "https://doi.org/10.1016/j.ygcen.2009.07.008" @default.
• W2115274299 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19607832" @default.
• W2115274299 hasPublicationYear "2010" @default.
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