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- W2115284875 abstract "Spectrofluorometric imaging microscopy is demonstrated in a confocal microscope using a supercontinuum laser as an excitation source and a custom-built prism spectrometer for detection. This microscope system provides confocal imaging with spectrally resolved fluorescence excitation and detection from 450 to 700 nm. The supercontinuum laser provides a broad spectrum light source and is coupled with an acousto-optic tunable filter to provide continuously tunable fluorescence excitation with a 1-nm bandwidth. Eight different excitation wavelengths can be simultaneously selected. The prism spectrometer provides spectrally resolved detection with sensitivity comparable to a standard confocal system. This new microscope system enables optimal access to a multitude of fluorophores and provides fluorescence excitation and emission spectra for each location in a 3D confocal image. The speed of the spectral scans is suitable for spectrofluorometric imaging of live cells. Effects of chromatic aberration are modest and do not significantly limit the spatial resolution of the confocal measurements." @default.
- W2115284875 created "2016-06-24" @default.
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- W2115284875 date "2007-09-01" @default.
- W2115284875 modified "2023-09-27" @default.
- W2115284875 title "A white light confocal microscope for spectrally resolved multidimensional imaging" @default.
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- W2115284875 doi "https://doi.org/10.1111/j.1365-2818.2007.01803.x" @default.
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