Matches in SemOpenAlex for { <https://semopenalex.org/work/W2115292582> ?p ?o ?g. }
- W2115292582 abstract "Methotrexate (MTX) was first introduced as a cytotoxic agent that inhibits nucleotide biosynthesis in various cancer disorders. Accumulating evidences suggest that MTX inhibits the proliferation of malignant cells by inhibiting 5-aminoimidazole-4-carbox-amide ribonucleotide transformylase, isoprenylcysteine carboxyl methyltransferase and NF-kappaB transcription factor. These observations indicate that MTX could have additional molecular targets that are therefore unappreciated. To get insights into the complex molecular mechanisms of MTX induced apoptosis in acute promyelocytic leukemia cells (HL 60), we conducted an investigation incorporating cysteine labeled differential in gel electrophoresis combined with mass spectrometry. Initial experimental analysis revealed that 24 proteins were differentially expressed (p > 0.05) in HL 60 cell proteome after addition of 2.5 µM MTX for 72 hours. The majority of MTX induced proteins were ascribed to the endoplasmic reticulum (ER) chaperones, glycolytic enzymes and the mitochondrial transmembrane electron transport system (MTETS). In particular, we noted that three structural alpha4, alpha5, alpha7; a non-catalytically beta3 and two 26S regulatory proteasome subunits were significantly down regulated in MTX treated HL 60 cell. We further showed in HL 60 cells that MTX induces ER chaperones, suppresses the NF-kappaB subunit p65, disturbs the mitochondrial transmembrane potential (Äøm) and generates reactive oxygen species (ROS) in a time dependent manner. All together, our findings revealed that MTX alter the level of a variety of proteins involved in the NF-kappaB associated proteasome complex formation, ER stress, and the MTETS. Their identification as molecular targets of MTX may provide new impulse in the understanding of apoptotic activities in acute promyelocytic leukaemia cell line." @default.
- W2115292582 created "2016-06-24" @default.
- W2115292582 creator A5017821768 @default.
- W2115292582 date "2022-02-20" @default.
- W2115292582 modified "2023-09-25" @default.
- W2115292582 title "Proteome-wide Identification of New Molecular Targets Affected by Methotrexate in Acute Promyelocytic Leukaemia Cell Line" @default.
- W2115292582 cites W125106425 @default.
- W2115292582 cites W140561711 @default.
- W2115292582 cites W14659763 @default.
- W2115292582 cites W1481925502 @default.
- W2115292582 cites W1487258982 @default.
- W2115292582 cites W1517396147 @default.
- W2115292582 cites W1527359370 @default.
- W2115292582 cites W1534776554 @default.
- W2115292582 cites W1558085296 @default.
- W2115292582 cites W1564896848 @default.
- W2115292582 cites W1565550299 @default.
- W2115292582 cites W1571989791 @default.
- W2115292582 cites W1579562694 @default.
- W2115292582 cites W1585517331 @default.
- W2115292582 cites W1610262691 @default.
- W2115292582 cites W161377905 @default.
- W2115292582 cites W1644953918 @default.
- W2115292582 cites W1773256867 @default.
- W2115292582 cites W1805258373 @default.
- W2115292582 cites W1832535988 @default.
- W2115292582 cites W188006079 @default.
- W2115292582 cites W1897325250 @default.
- W2115292582 cites W1940692702 @default.
- W2115292582 cites W1948197084 @default.
- W2115292582 cites W1964313477 @default.
- W2115292582 cites W1967660811 @default.
- W2115292582 cites W1968028322 @default.
- W2115292582 cites W1971415727 @default.
- W2115292582 cites W1972733192 @default.
- W2115292582 cites W1973962332 @default.
- W2115292582 cites W1976582166 @default.
- W2115292582 cites W1976881265 @default.
- W2115292582 cites W1977402193 @default.
- W2115292582 cites W1979129905 @default.
- W2115292582 cites W1981593008 @default.
- W2115292582 cites W1981630094 @default.
- W2115292582 cites W1982721874 @default.
- W2115292582 cites W1985096167 @default.
- W2115292582 cites W1988356885 @default.
- W2115292582 cites W1988502798 @default.
- W2115292582 cites W1989738506 @default.
- W2115292582 cites W1991354483 @default.
- W2115292582 cites W1992423687 @default.
- W2115292582 cites W1992497040 @default.
- W2115292582 cites W1993378834 @default.
- W2115292582 cites W1995191054 @default.
- W2115292582 cites W1995945569 @default.
- W2115292582 cites W1996620981 @default.
- W2115292582 cites W2001756549 @default.
- W2115292582 cites W2002267072 @default.
- W2115292582 cites W2002408013 @default.
- W2115292582 cites W2002587784 @default.
- W2115292582 cites W2003844583 @default.
- W2115292582 cites W2005756814 @default.
- W2115292582 cites W2006929818 @default.
- W2115292582 cites W2008898220 @default.
- W2115292582 cites W201163751 @default.
- W2115292582 cites W2012744772 @default.
- W2115292582 cites W2014842408 @default.
- W2115292582 cites W2015732113 @default.
- W2115292582 cites W2015787260 @default.
- W2115292582 cites W2019836238 @default.
- W2115292582 cites W2020796595 @default.
- W2115292582 cites W2024021696 @default.
- W2115292582 cites W2026525843 @default.
- W2115292582 cites W2026642838 @default.
- W2115292582 cites W2027716292 @default.
- W2115292582 cites W2028625808 @default.
- W2115292582 cites W2029642537 @default.
- W2115292582 cites W2031443707 @default.
- W2115292582 cites W2031495220 @default.
- W2115292582 cites W2034207498 @default.
- W2115292582 cites W2043634641 @default.
- W2115292582 cites W2044320834 @default.
- W2115292582 cites W2047201865 @default.
- W2115292582 cites W2047591593 @default.
- W2115292582 cites W2047861739 @default.
- W2115292582 cites W2051612655 @default.
- W2115292582 cites W2052607700 @default.
- W2115292582 cites W2054210423 @default.
- W2115292582 cites W2054794987 @default.
- W2115292582 cites W2062709319 @default.
- W2115292582 cites W2063183292 @default.
- W2115292582 cites W2064051300 @default.
- W2115292582 cites W2064576240 @default.
- W2115292582 cites W2067653691 @default.
- W2115292582 cites W2067709198 @default.
- W2115292582 cites W2068696470 @default.
- W2115292582 cites W207029473 @default.
- W2115292582 cites W2071425667 @default.
- W2115292582 cites W2076372887 @default.
- W2115292582 cites W2077288249 @default.
- W2115292582 cites W2081699153 @default.
- W2115292582 cites W2083361186 @default.