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• W2115295625 abstract "<h3>Abstract</h3> The bacterial genus <i>Xylella</i> contains plant pathogens that are major threats to agriculture worldwide. Although extensive research was conducted to characterize different subspecies of <i>Xylella fastidiosa</i> (<i>Xf</i>), comparative analysis at above-species levels were lacking due to the unavailability of appropriate data sets. Recently, a bacterium that causes pear leaf scorch (PLS) in Taiwan was described as the second <i>Xylella</i> species (i.e., <i>Xylella taiwanensis</i>; <i>Xt</i>). In this work, we report the complete genome sequence of <i>Xt</i> type strain PLS229^T. The genome-scale phylogeny provided strong support that <i>Xf</i> subspecies <i>pauca</i> (<i>Xfp</i>) is the basal lineage of this species and <i>Xylella</i> was derived from the paraphyletic genus <i>Xanthomonas</i>. Quantification of genomic divergence indicated that different <i>Xf</i> subspecies share ~87-95% of their genomic segments, while the two <i>Xylella</i> species share only ~66-70%. Analysis of overall gene content suggested that <i>Xt</i> is most similar to <i>Xf</i> subspecies <i>sandyi</i> (<i>Xfs</i>). Based on the existing knowledge of <i>Xf</i> virulence genes, the homolog distribution among 28 <i>Xylella</i> representatives was examined. Among the 10 functional categories, those involved in secretion, metabolism, and stress response are the most conserved ones with no copy number variation. In contrast, several genes related to adhesins, hydrolytic enzymes, and toxin-antitoxin systems are highly variable in their copy numbers. Those virulence genes with high levels of conservation or variation may be promising candidates for future studies. In summary, the new genome sequence and analysis reported in this work contributed to the study of several important pathogens in the family Xanthomonadaceae." @default.
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• W2115295625 date "2012-08-13" @default.
• W2115295625 modified "2023-10-05" @default.
• W2115295625 title "If you ask the wrong question, you'll get the wrong answer" @default.
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• W2115295625 doi "https://doi.org/10.1136/medethics-2012-100682" @default.
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