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- W2115386301 abstract "Translational activation of mRNAs stored in the cytoplasm is a key process in the regulation of gene expression during spermatogenesis. Embryonic poly(A) binding protein (ePAB) is the predominant poly(A) binding protein during oocyte and early embryo development until ZGA; it stabilizes mRNAs and promotes their translation. We hypothesized that ePAB plays a key role in spermatogenesis and tested our hypothesis by generating and characterizing mice homozygous-deficient for ePAB. Experimental study. ePAB knockout (ePAB-/-) mice were generated by targeted deletion of ePAB exon 2. Fertility of wild type (WT), heterozygous knockout (ePAB+/-), and homozygous knockout (ePAB-/-) male mice (n = 12 for each genotype) was tested by mating with 5 adult WT female mice with proven fertility (male:female; 1:2), for a total of 20 weeks (minimum 4 oestrus cycles with each male). Testis weight and histology, and sperm count and morphology were evaluated. Apoptotic index in spermatozoa was determined by annexin V staining. Expression of RNA-binding proteins MSY2, PAIP2, PUM2, and PABPC1 in testis of WT and ePAB-/- mice (n = 5 in each group) was determined by real-time polymerase chain reaction (qRT-PCR) and normalized to actin expression. Male ePAB-/- mice were viable, appeared phenotypically normal, and demonstrated normal fertility. Testis weight, seminiferous tubule diameter, sperm count, sperm morphology, or annexin V staining were not different between WT and ePAB-/- mice. MSY2 expression was increased by 50% in ePAB-/- mice (P<0.05). Levels of other RNA-binding proteins tested were also elevated (10-40%) in testes of ePAB-/- mice but the differences were not statistically significant. We found that male ePAB-/- mice are fertile, and have normal testis histology and sperm parameters. Elevated MSY2 and possibly other RNA-binding proteins seem to compensate for the required RNA-binding activity in testes of ePAB-/- mice." @default.
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- W2115386301 date "2011-09-01" @default.
- W2115386301 modified "2023-09-30" @default.
- W2115386301 title "Embryonic poly(A) binding protein (ePAB)-deficient male mice have elevated MSY2 expression in testis" @default.
- W2115386301 doi "https://doi.org/10.1016/j.fertnstert.2011.07.339" @default.
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