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• W2115519265 endingPage "941" @default.
• W2115519265 startingPage "931" @default.
• W2115519265 abstract "ABSTRACT Balancing the flux of a heterologous metabolic pathway by tuning the expression and properties of the pathway enzymes is difficult, but it is critical to realizing the full potential of microbial biotechnology. One prominent example is the metabolic engineering of a Saccharomyces cerevisiae strain harboring a heterologous xylose-utilizing pathway for cellulosic-biofuel production, which remains a challenge even after decades of research. Here, we developed a combinatorial pathway-engineering approach to rapidly create a highly efficient xylose-utilizing pathway for ethanol production by exploring various combinations of enzyme homologues with different properties. A library of more than 8,000 xylose utilization pathways was generated using DNA assembler, followed by multitiered screening, which led to the identification of a number of strain-specific combinations of the enzymes for efficient conversion of xylose to ethanol. The balancing of metabolic flux through the xylose utilization pathway was demonstrated by a complete reversal of the major product from xylitol to ethanol with a similar yield and total by-product formation as low as 0.06 g/g xylose without compromising cell growth. The results also suggested that an optimal enzyme combination depends on not only the genotype/phenotype of the host strain, but also the sugar composition of the fermentation medium. This combinatorial approach should be applicable to any heterologous pathway and will be instrumental in the optimization of industrial production of value-added products." @default.
• W2115519265 created "2016-06-24" @default.
• W2115519265 creator A5014465828 @default.
• W2115519265 creator A5024169462 @default.
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• W2115519265 date "2013-02-01" @default.
• W2115519265 modified "2023-10-18" @default.
• W2115519265 title "Combinatorial Design of a Highly Efficient Xylose-Utilizing Pathway in Saccharomyces cerevisiae for the Production of Cellulosic Biofuels" @default.
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• W2115519265 doi "https://doi.org/10.1128/aem.02736-12" @default.
• W2115519265 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3568569" @default.
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• W2115519265 hasPublicationYear "2013" @default.
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