FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2115527117> ?p ?o ?g. }

• W2115527117 endingPage "501" @default.
• W2115527117 startingPage "501" @default.
• W2115527117 abstract "Copper plays essential roles in biology, but abnormal interactions are damaging. Reliable quantification of copper-protein interactions will underpin the molecular understanding of copper nutrition and toxicity. We have previously established two high affinity probes, Bathocuproine disulfonate (Bcs) and Bicinchoninate (Bca) anions, that are capable of in vitro quantification of Cu(i) binding with affinities from pico- to atto-molar concentrations. Quantitative probes are required for Cu(i) binding of lower affinity for proteins and peptides typically associated with neurodegenerative diseases. The present work evaluates two classic Fe(ii) ligands Ferene S (Fs) and Ferrozine (Fz) as quantitative probes for Cu(i). Both react with Cu(i) quantitatively to yield well-defined complex anions [Cu(I)(Fs)2](3-) (λmax = 484 nm, ε = 6700 cm(-1) M(-1)) and [Cu(I)(Fz)2](3-) (λmax = 470 nm, ε = 4320 cm(-1) M(-1)). These complexes are sensitive to aerial oxidation (E1/2∼ +0.36 V vs. SHE) and to substitution by other ligands (e.g., Cl(-), MeCN). However, they can be protected effectively under anaerobic conditions by suitable reductants and an excess of the free probe ligands. Formation constants β2 were determined by two approaches: direct metal ion titration and ligand competition. They provided estimates which differed by ∼3 orders of magnitude. The sources of these differences were examined carefully to consolidate the affinities of the two probes to a unified standard (10(15.1) M(-2) for Fz and 10(13.7) M(-2) for Fs). It is apparent that application of direct metal ion titrations to quantification of Cu(i) binding affinities is problematical and should be avoided. The four ligands Bcs, Bca, Fz and Fs in combination form a set of versatile probes for ligand competition experiments and are capable of detecting and differentiating an extended spectrum of Cu(i) binding affinities from nano- to atto-molar concentrations. Selected examples of quantification of weaker Cu(i) binding in proteins and peptides are provided, including that of an amyloid-β peptide." @default.
• W2115527117 created "2016-06-24" @default.
• W2115527117 creator A5020333330 @default.
• W2115527117 creator A5029281644 @default.
• W2115527117 creator A5045117180 @default.
• W2115527117 creator A5066854381 @default.
• W2115527117 creator A5076044886 @default.
• W2115527117 date "2013-01-01" @default.
• W2115527117 modified "2023-10-14" @default.
• W2115527117 title "Evaluation of quantitative probes for weaker Cu(i) binding sites completes a set of four capable of detecting Cu(i) affinities from nanomolar to attomolar" @default.
• W2115527117 cites W1791674767 @default.
• W2115527117 cites W1869259273 @default.
• W2115527117 cites W1966618264 @default.
• W2115527117 cites W1966732500 @default.
• W2115527117 cites W1969364897 @default.
• W2115527117 cites W1970503230 @default.
• W2115527117 cites W1980099457 @default.
• W2115527117 cites W1991039509 @default.
• W2115527117 cites W1997565922 @default.
• W2115527117 cites W1997854913 @default.
• W2115527117 cites W2006946612 @default.
• W2115527117 cites W2008516264 @default.
• W2115527117 cites W2013801677 @default.
• W2115527117 cites W2015564773 @default.
• W2115527117 cites W2019279182 @default.
• W2115527117 cites W2034520333 @default.
• W2115527117 cites W2039161703 @default.
• W2115527117 cites W2041055869 @default.
• W2115527117 cites W2041846728 @default.
• W2115527117 cites W2042897050 @default.
• W2115527117 cites W2042923123 @default.
• W2115527117 cites W2043104518 @default.
• W2115527117 cites W2044627978 @default.
• W2115527117 cites W2045969222 @default.
• W2115527117 cites W2046156129 @default.
• W2115527117 cites W2051986914 @default.
• W2115527117 cites W2052607444 @default.
• W2115527117 cites W2053327937 @default.
• W2115527117 cites W2062820102 @default.
• W2115527117 cites W2067322609 @default.
• W2115527117 cites W2072603543 @default.
• W2115527117 cites W2073057315 @default.
• W2115527117 cites W2081456258 @default.
• W2115527117 cites W2084123810 @default.
• W2115527117 cites W2087875520 @default.
• W2115527117 cites W2088616035 @default.
• W2115527117 cites W2091913250 @default.
• W2115527117 cites W2106301585 @default.
• W2115527117 cites W2119023359 @default.
• W2115527117 cites W2129731711 @default.
• W2115527117 cites W2139538779 @default.
• W2115527117 cites W2154010267 @default.
• W2115527117 cites W2315218909 @default.
• W2115527117 cites W2315760675 @default.
• W2115527117 cites W2316955898 @default.
• W2115527117 cites W2330361286 @default.
• W2115527117 cites W4243940701 @default.
• W2115527117 doi "https://doi.org/10.1039/c3mt00032j" @default.
• W2115527117 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23579336" @default.
• W2115527117 hasPublicationYear "2013" @default.
• W2115527117 type Work @default.
• W2115527117 sameAs 2115527117 @default.
• W2115527117 citedByCount "61" @default.
• W2115527117 countsByYear W21155271172013 @default.
• W2115527117 countsByYear W21155271172014 @default.
• W2115527117 countsByYear W21155271172015 @default.
• W2115527117 countsByYear W21155271172016 @default.
• W2115527117 countsByYear W21155271172017 @default.
• W2115527117 countsByYear W21155271172018 @default.
• W2115527117 countsByYear W21155271172019 @default.
• W2115527117 countsByYear W21155271172020 @default.
• W2115527117 countsByYear W21155271172021 @default.
• W2115527117 countsByYear W21155271172022 @default.
• W2115527117 countsByYear W21155271172023 @default.
• W2115527117 crossrefType "journal-article" @default.
• W2115527117 hasAuthorship W2115527117A5020333330 @default.
• W2115527117 hasAuthorship W2115527117A5029281644 @default.
• W2115527117 hasAuthorship W2115527117A5045117180 @default.
• W2115527117 hasAuthorship W2115527117A5066854381 @default.
• W2115527117 hasAuthorship W2115527117A5076044886 @default.
• W2115527117 hasBestOaLocation W21155271171 @default.
• W2115527117 hasConcept C116569031 @default.
• W2115527117 hasConcept C170493617 @default.
• W2115527117 hasConcept C178790620 @default.
• W2115527117 hasConcept C179104552 @default.
• W2115527117 hasConcept C184866935 @default.
• W2115527117 hasConcept C185592680 @default.
• W2115527117 hasConcept C199164860 @default.
• W2115527117 hasConcept C2780283098 @default.
• W2115527117 hasConcept C3018795828 @default.
• W2115527117 hasConcept C3019856697 @default.
• W2115527117 hasConcept C544153396 @default.
• W2115527117 hasConcept C544778455 @default.
• W2115527117 hasConcept C55493867 @default.
• W2115527117 hasConcept C71240020 @default.
• W2115527117 hasConcept C8010536 @default.
• W2115527117 hasConceptScore W2115527117C116569031 @default.
• W2115527117 hasConceptScore W2115527117C170493617 @default.

• next