FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2115536254> ?p ?o ?g. }

• W2115536254 endingPage "35" @default.
• W2115536254 startingPage "31" @default.
• W2115536254 abstract "Current techniques of sperm preparation for in vitro fertilization or intrauterine insemination require centrifugation of human semen to separate spermatozoa from the seminal plasma. Centrifugation increases reactive oxygen species (ROS) formation in semen. Moreover, high levels of ROS are associated with sperm membrane injury through spontaneous lipid peroxidation, which may alter sperm function. We investigated the relationship between centrifugation variables (time and g-force) and ROS production to establish an optimal centrifugation protocol for sperm preparation techniques. Semen from 38 men (24 patients and 14 normal volunteers) was evaluated for the formation of ROS before centrifugation and after centrifugation at 200 g for 2 or 10 min and after 500 g for 2 or 10 min. The absence of white blood cells in semen which can also produce ROS was determined with the myeloperoxidase technique (Endtz test). All specimens were negative (< 1 x 10^6/ml) by the Endtz test. The formation of ROS was measured by chemiluminescence. ROS formation was regarded as high (positive) when the chemiluminescence response was at least 10 x 10^4 counted photons/min (cpm). The sperm concentration in each sample was adjusted to 15-20 x 10^6 cells/ml before analysis. Eight specimens (7 patients and 1 donor) exhibited high levels of ROS before centrifugation. All 8 showed further, significant increases in ROS formation regardless of g-force or time. The increase in ROS was significantly less when semen was centrifuged for 2 as compared to 10 min (p < 0.001). Six specimens previously ROS-negative became ROS-positive after centrifugation for 10 min at 200 and 500 g. We conclude that the time of centrifugation is more important than force for inducing ROS formation in semen. Based on these results, we recommend a shorter centrifugation period in the preparation of sperm for assisted reproductive techniques." @default.
• W2115536254 created "2016-06-24" @default.
• W2115536254 creator A5006625551 @default.
• W2115536254 creator A5016488532 @default.
• W2115536254 creator A5025063876 @default.
• W2115536254 creator A5034076640 @default.
• W2115536254 date "1995-01-01" @default.
• W2115536254 modified "2023-09-30" @default.
• W2115536254 title "A Method of Human Semen Centrifugation to Minimize the Iatrogenic Sperm Injuries Caused by Reactive Oxygen Species" @default.
• W2115536254 cites W1561433308 @default.
• W2115536254 cites W1684021943 @default.
• W2115536254 cites W1775349051 @default.
• W2115536254 cites W1912079946 @default.
• W2115536254 cites W1973256172 @default.
• W2115536254 cites W1983662609 @default.
• W2115536254 cites W1994291765 @default.
• W2115536254 cites W2003188074 @default.
• W2115536254 cites W2039839257 @default.
• W2115536254 cites W2046332402 @default.
• W2115536254 cites W2128718884 @default.
• W2115536254 cites W2141462357 @default.
• W2115536254 cites W2164809374 @default.
• W2115536254 cites W2272838734 @default.
• W2115536254 cites W2342601641 @default.
• W2115536254 cites W2408906108 @default.
• W2115536254 cites W2415568802 @default.
• W2115536254 cites W2415606380 @default.
• W2115536254 cites W2615205751 @default.
• W2115536254 doi "https://doi.org/10.1159/000475016" @default.
• W2115536254 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8521891" @default.
• W2115536254 hasPublicationYear "1995" @default.
• W2115536254 type Work @default.
• W2115536254 sameAs 2115536254 @default.
• W2115536254 citedByCount "137" @default.
• W2115536254 countsByYear W21155362542012 @default.
• W2115536254 countsByYear W21155362542013 @default.
• W2115536254 countsByYear W21155362542014 @default.
• W2115536254 countsByYear W21155362542015 @default.
• W2115536254 countsByYear W21155362542016 @default.
• W2115536254 countsByYear W21155362542017 @default.
• W2115536254 countsByYear W21155362542018 @default.
• W2115536254 countsByYear W21155362542019 @default.
• W2115536254 countsByYear W21155362542020 @default.
• W2115536254 countsByYear W21155362542021 @default.
• W2115536254 countsByYear W21155362542022 @default.
• W2115536254 countsByYear W21155362542023 @default.
• W2115536254 crossrefType "journal-article" @default.
• W2115536254 hasAuthorship W2115536254A5006625551 @default.
• W2115536254 hasAuthorship W2115536254A5016488532 @default.
• W2115536254 hasAuthorship W2115536254A5025063876 @default.
• W2115536254 hasAuthorship W2115536254A5034076640 @default.
• W2115536254 hasConcept C123765429 @default.
• W2115536254 hasConcept C132946820 @default.
• W2115536254 hasConcept C16685009 @default.
• W2115536254 hasConcept C185592680 @default.
• W2115536254 hasConcept C203014093 @default.
• W2115536254 hasConcept C2777005246 @default.
• W2115536254 hasConcept C2777589648 @default.
• W2115536254 hasConcept C2777688143 @default.
• W2115536254 hasConcept C2778093475 @default.
• W2115536254 hasConcept C2779234561 @default.
• W2115536254 hasConcept C2781087480 @default.
• W2115536254 hasConcept C48349386 @default.
• W2115536254 hasConcept C54355233 @default.
• W2115536254 hasConcept C55493867 @default.
• W2115536254 hasConcept C71924100 @default.
• W2115536254 hasConcept C7623868 @default.
• W2115536254 hasConcept C86803240 @default.
• W2115536254 hasConceptScore W2115536254C123765429 @default.
• W2115536254 hasConceptScore W2115536254C132946820 @default.
• W2115536254 hasConceptScore W2115536254C16685009 @default.
• W2115536254 hasConceptScore W2115536254C185592680 @default.
• W2115536254 hasConceptScore W2115536254C203014093 @default.
• W2115536254 hasConceptScore W2115536254C2777005246 @default.
• W2115536254 hasConceptScore W2115536254C2777589648 @default.
• W2115536254 hasConceptScore W2115536254C2777688143 @default.
• W2115536254 hasConceptScore W2115536254C2778093475 @default.
• W2115536254 hasConceptScore W2115536254C2779234561 @default.
• W2115536254 hasConceptScore W2115536254C2781087480 @default.
• W2115536254 hasConceptScore W2115536254C48349386 @default.
• W2115536254 hasConceptScore W2115536254C54355233 @default.
• W2115536254 hasConceptScore W2115536254C55493867 @default.
• W2115536254 hasConceptScore W2115536254C71924100 @default.
• W2115536254 hasConceptScore W2115536254C7623868 @default.
• W2115536254 hasConceptScore W2115536254C86803240 @default.
• W2115536254 hasIssue "1" @default.
• W2115536254 hasLocation W21155362541 @default.
• W2115536254 hasLocation W21155362542 @default.
• W2115536254 hasOpenAccess W2115536254 @default.
• W2115536254 hasPrimaryLocation W21155362541 @default.
• W2115536254 hasRelatedWork W1973007694 @default.
• W2115536254 hasRelatedWork W2063897841 @default.
• W2115536254 hasRelatedWork W2133709357 @default.
• W2115536254 hasRelatedWork W2170230964 @default.
• W2115536254 hasRelatedWork W2976439174 @default.
• W2115536254 hasRelatedWork W3030290948 @default.
• W2115536254 hasRelatedWork W3044546043 @default.
• W2115536254 hasRelatedWork W4256147281 @default.

• next