Matches in SemOpenAlex for { <https://semopenalex.org/work/W2115538351> ?p ?o ?g. }
- W2115538351 endingPage "752" @default.
- W2115538351 startingPage "742" @default.
- W2115538351 abstract "Abstract High throughput sequencing methods are widely used in analyses of microbial diversity, but are generally applied to small numbers of samples, which precludes characterization of patterns of microbial diversity across space and time. We have designed a primer‐tagging approach that allows pooling and subsequent sorting of numerous samples, which is directed to amplification of a region spanning the nuclear ribosomal internal transcribed spacers and partial large subunit from fungi in environmental samples. To test the method for phylogenetic biases, we constructed a controlled mixture of four taxa representing the Chytridiomycota, Zygomycota, Ascomycota and Basidiomycota. Following cloning and colony restriction fragment length polymorphism analysis, we found no significant difference in representation in 19 of the 23 tested primers. We also generated a clone library from two soil DNA extracts using two primers for each extract and compared 456 clone sequences. Community diversity statistics and contingency table tests applied to counts of operational taxonomic units revealed that the two DNA extracts differed significantly, while the pairs of tagged primers from each extract were indistinguishable. Similar results were obtained using UniFrac phylogenetic comparisons. Together, these results suggest that the pig‐tagged primers can be used to increase ecological inference in high throughput sequencing projects on fungi." @default.
- W2115538351 created "2016-06-24" @default.
- W2115538351 creator A5008887936 @default.
- W2115538351 creator A5049581540 @default.
- W2115538351 creator A5062762227 @default.
- W2115538351 creator A5067030140 @default.
- W2115538351 creator A5068582634 @default.
- W2115538351 creator A5088076006 @default.
- W2115538351 creator A5090619209 @default.
- W2115538351 date "2008-06-28" @default.
- W2115538351 modified "2023-10-13" @default.
- W2115538351 title "Increasing ecological inference from high throughput sequencing of fungi in the environment through a tagging approach" @default.
- W2115538351 cites W1559067994 @default.
- W2115538351 cites W1598574080 @default.
- W2115538351 cites W182620622 @default.
- W2115538351 cites W1965937322 @default.
- W2115538351 cites W1968358911 @default.
- W2115538351 cites W1973439073 @default.
- W2115538351 cites W1994753577 @default.
- W2115538351 cites W2021438117 @default.
- W2115538351 cites W2043401730 @default.
- W2115538351 cites W2046217121 @default.
- W2115538351 cites W2060566127 @default.
- W2115538351 cites W2069794768 @default.
- W2115538351 cites W2070479548 @default.
- W2115538351 cites W2073032598 @default.
- W2115538351 cites W2075269024 @default.
- W2115538351 cites W2078872583 @default.
- W2115538351 cites W2090795003 @default.
- W2115538351 cites W2094591409 @default.
- W2115538351 cites W2101272820 @default.
- W2115538351 cites W2102555068 @default.
- W2115538351 cites W2105837826 @default.
- W2115538351 cites W2108424938 @default.
- W2115538351 cites W2113915829 @default.
- W2115538351 cites W2120108422 @default.
- W2115538351 cites W2120472610 @default.
- W2115538351 cites W2122880809 @default.
- W2115538351 cites W2128114769 @default.
- W2115538351 cites W2130889389 @default.
- W2115538351 cites W2132159473 @default.
- W2115538351 cites W2136233546 @default.
- W2115538351 cites W2139595361 @default.
- W2115538351 cites W2140341891 @default.
- W2115538351 cites W2140770632 @default.
- W2115538351 cites W2142013469 @default.
- W2115538351 cites W2145166062 @default.
- W2115538351 cites W2151141101 @default.
- W2115538351 cites W2156465688 @default.
- W2115538351 cites W2156980465 @default.
- W2115538351 cites W2158421363 @default.
- W2115538351 cites W2161817666 @default.
- W2115538351 cites W2162505054 @default.
- W2115538351 cites W2162578061 @default.
- W2115538351 cites W2164628641 @default.
- W2115538351 cites W2165065263 @default.
- W2115538351 cites W2166265186 @default.
- W2115538351 cites W2167571746 @default.
- W2115538351 cites W2170024099 @default.
- W2115538351 cites W2313677986 @default.
- W2115538351 cites W2415046415 @default.
- W2115538351 doi "https://doi.org/10.1111/j.1755-0998.2008.02094.x" @default.
- W2115538351 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21585882" @default.
- W2115538351 hasPublicationYear "2008" @default.
- W2115538351 type Work @default.
- W2115538351 sameAs 2115538351 @default.
- W2115538351 citedByCount "49" @default.
- W2115538351 countsByYear W21155383512012 @default.
- W2115538351 countsByYear W21155383512013 @default.
- W2115538351 countsByYear W21155383512014 @default.
- W2115538351 countsByYear W21155383512015 @default.
- W2115538351 countsByYear W21155383512016 @default.
- W2115538351 countsByYear W21155383512017 @default.
- W2115538351 countsByYear W21155383512018 @default.
- W2115538351 countsByYear W21155383512019 @default.
- W2115538351 countsByYear W21155383512021 @default.
- W2115538351 countsByYear W21155383512022 @default.
- W2115538351 crossrefType "journal-article" @default.
- W2115538351 hasAuthorship W2115538351A5008887936 @default.
- W2115538351 hasAuthorship W2115538351A5049581540 @default.
- W2115538351 hasAuthorship W2115538351A5062762227 @default.
- W2115538351 hasAuthorship W2115538351A5067030140 @default.
- W2115538351 hasAuthorship W2115538351A5068582634 @default.
- W2115538351 hasAuthorship W2115538351A5088076006 @default.
- W2115538351 hasAuthorship W2115538351A5090619209 @default.
- W2115538351 hasConcept C104317684 @default.
- W2115538351 hasConcept C125009961 @default.
- W2115538351 hasConcept C1436000 @default.
- W2115538351 hasConcept C151437367 @default.
- W2115538351 hasConcept C155794727 @default.
- W2115538351 hasConcept C193252679 @default.
- W2115538351 hasConcept C207812311 @default.
- W2115538351 hasConcept C2781212518 @default.
- W2115538351 hasConcept C42062724 @default.
- W2115538351 hasConcept C49105822 @default.
- W2115538351 hasConcept C51679486 @default.
- W2115538351 hasConcept C523546767 @default.
- W2115538351 hasConcept C54355233 @default.