Matches in SemOpenAlex for { <https://semopenalex.org/work/W2115580820> ?p ?o ?g. }
- W2115580820 endingPage "87" @default.
- W2115580820 startingPage "77" @default.
- W2115580820 abstract "DNA microarrays can provide information about the expression levels of thousands of genes simultaneously at the transcriptomic level, while conventional cell viability and cytotoxicity measurement methods provide information about the biological functions at the cellular level. Integrating these data at different levels provides a promising approach for evaluating or predicting how cells respond to chemical exposure. It is important to investigate the multi-scale biological system in a systematic way to better understand the gene regulation networks and signal transduction pathways involved in the cellular responses to environmental factors.Primary rat hepatocytes were exposed to cadmium acetate at 0, 1.25 and 2 microM. mRNA expression profiles at 0, 3, 6, 12 and 24 h were measured using the Affymetrix RatTox U34 GeneChip arrays. Simultaneously, cytotoxicity was assessed by lactase dehydrogenase leakage assay. Gene expression profiles at different time points were used to evaluate cytotoxicity at subsequent time points using partial least squares, and it was found that gene expression profiles at 0 h had the best prediction accuracy for the cytotoxicity observed at 12 h. Some biomarkers whose expression profiles showed strong relationship with cytotoxicity were identified and the underlying pathways were reconstructed to illustrate how hepatocytes respond to cadmium exposure. Permutation studies were also applied to assess the reliability of the predictive models.Matlab source code is available upon request and DNA microarray data are available at GEO (http://www.ncbi.nlm.nih.gov/geo)." @default.
- W2115580820 created "2016-06-24" @default.
- W2115580820 creator A5040900307 @default.
- W2115580820 creator A5043439155 @default.
- W2115580820 creator A5068886380 @default.
- W2115580820 creator A5070489210 @default.
- W2115580820 creator A5078638755 @default.
- W2115580820 creator A5088070159 @default.
- W2115580820 creator A5089216875 @default.
- W2115580820 date "2005-10-25" @default.
- W2115580820 modified "2023-10-01" @default.
- W2115580820 title "Integrating time-course microarray gene expression profiles with cytotoxicity for identification of biomarkers in primary rat hepatocytes exposed to cadmium" @default.
- W2115580820 cites W1566737177 @default.
- W2115580820 cites W1584846756 @default.
- W2115580820 cites W1597011288 @default.
- W2115580820 cites W1900912696 @default.
- W2115580820 cites W1966089218 @default.
- W2115580820 cites W1967053372 @default.
- W2115580820 cites W1968160256 @default.
- W2115580820 cites W1971059223 @default.
- W2115580820 cites W1971278063 @default.
- W2115580820 cites W1973614704 @default.
- W2115580820 cites W1975219953 @default.
- W2115580820 cites W1978941462 @default.
- W2115580820 cites W1979612645 @default.
- W2115580820 cites W1980084260 @default.
- W2115580820 cites W1983536745 @default.
- W2115580820 cites W1989539998 @default.
- W2115580820 cites W1991680649 @default.
- W2115580820 cites W1997656209 @default.
- W2115580820 cites W2005900169 @default.
- W2115580820 cites W2011424178 @default.
- W2115580820 cites W2012384644 @default.
- W2115580820 cites W2013485244 @default.
- W2115580820 cites W2016157964 @default.
- W2115580820 cites W2019001895 @default.
- W2115580820 cites W2019107186 @default.
- W2115580820 cites W2020286598 @default.
- W2115580820 cites W2021551481 @default.
- W2115580820 cites W2022441134 @default.
- W2115580820 cites W2024390537 @default.
- W2115580820 cites W2024591653 @default.
- W2115580820 cites W2025128734 @default.
- W2115580820 cites W2025206606 @default.
- W2115580820 cites W2025679749 @default.
- W2115580820 cites W2026403124 @default.
- W2115580820 cites W2034544271 @default.
- W2115580820 cites W2035530476 @default.
- W2115580820 cites W2043235003 @default.
- W2115580820 cites W2043483837 @default.
- W2115580820 cites W2045309540 @default.
- W2115580820 cites W2045336785 @default.
- W2115580820 cites W2045338351 @default.
- W2115580820 cites W2049312704 @default.
- W2115580820 cites W2049378629 @default.
- W2115580820 cites W2050268016 @default.
- W2115580820 cites W2057184774 @default.
- W2115580820 cites W2058357865 @default.
- W2115580820 cites W2060211824 @default.
- W2115580820 cites W2062832120 @default.
- W2115580820 cites W2073503722 @default.
- W2115580820 cites W2074227902 @default.
- W2115580820 cites W2076807689 @default.
- W2115580820 cites W2081981245 @default.
- W2115580820 cites W2085827128 @default.
- W2115580820 cites W2089718934 @default.
- W2115580820 cites W2093875241 @default.
- W2115580820 cites W2095809779 @default.
- W2115580820 cites W2096158882 @default.
- W2115580820 cites W2100560815 @default.
- W2115580820 cites W2100946087 @default.
- W2115580820 cites W2105089690 @default.
- W2115580820 cites W2106914354 @default.
- W2115580820 cites W2107956883 @default.
- W2115580820 cites W2109363337 @default.
- W2115580820 cites W2112360818 @default.
- W2115580820 cites W2117998622 @default.
- W2115580820 cites W2119273131 @default.
- W2115580820 cites W2122128696 @default.
- W2115580820 cites W2122649297 @default.
- W2115580820 cites W2123672572 @default.
- W2115580820 cites W2127117261 @default.
- W2115580820 cites W2129895818 @default.
- W2115580820 cites W2130114526 @default.
- W2115580820 cites W2132752481 @default.
- W2115580820 cites W2133037422 @default.
- W2115580820 cites W2133199783 @default.
- W2115580820 cites W2138550913 @default.
- W2115580820 cites W2140689677 @default.
- W2115580820 cites W2142524412 @default.
- W2115580820 cites W2160311563 @default.
- W2115580820 cites W2165093044 @default.
- W2115580820 cites W2166446427 @default.
- W2115580820 cites W2168117806 @default.
- W2115580820 cites W2169980516 @default.
- W2115580820 cites W2171062114 @default.
- W2115580820 cites W2172249877 @default.
- W2115580820 cites W2214362811 @default.