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- W2115619308 abstract "Several of the aspartic acid protease inhibitors used to treat HIV infection increase basal lipolysis in adipocytes, but the cellular mechanisms leading to this augmentation are not well understood. We therefore studied the effects of chronic exposure to the HIV protease inhibitor, ritonavir, on the lipolytic cascade in 3T3-L1 adipocytes. Treatment of 3T3-L1 adipocytes with ritonavir for 14 d (during and after differentiation) enhanced basal, isoproterenol (Iso)-stimulated, and cAMP analog-stimulated lipolysis. Enhancement of lipolysis was observed after Iso at concentrations between 0.1 and 10 μm. Despite a significant decrease in cyclic nucleotide phosphodiesterase (PDE)3B activity and protein levels, there were no changes in Iso-stimulated intracellular cAMP, protein kinase A (PKA) expression, or PKA activity. Ritonavir-augmented lipolysis was also observed under conditions that reversed the effect on PDE3B activity via preincubation with 1 μm (-)-N6-(2-phenylisopropyl)adenosine. In ritonavir-treated cells, protein expression of the lipid droplet-protective protein, perilipin, was significantly decreased, whereas there was no change in hormone-sensitive lipase. Activation of ERK1/2 by Iso did not play a role in the augmentation. We conclude that ritonavir decreases PDE3B and perilipin protein expression and affects both basal and catecholamine-stimulated lipolysis in 3T3-L1 adipocytes primarily through actions at sites downstream of PKA." @default.
- W2115619308 created "2016-06-24" @default.
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- W2115619308 date "2005-06-01" @default.
- W2115619308 modified "2023-10-18" @default.
- W2115619308 title "Effects of the Human Immunodeficiency Virus-Protease Inhibitor, Ritonavir, on Basal and Catecholamine-Stimulated Lipolysis" @default.
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- W2115619308 cites W1504753880 @default.
- W2115619308 cites W1574301078 @default.
- W2115619308 cites W1593931056 @default.
- W2115619308 cites W1738497100 @default.
- W2115619308 cites W1757478802 @default.
- W2115619308 cites W1945140151 @default.
- W2115619308 cites W1967632729 @default.
- W2115619308 cites W1978444406 @default.
- W2115619308 cites W1980111447 @default.
- W2115619308 cites W1986618571 @default.
- W2115619308 cites W1994969848 @default.
- W2115619308 cites W1998661299 @default.
- W2115619308 cites W2001349528 @default.
- W2115619308 cites W2003403009 @default.
- W2115619308 cites W2004635464 @default.
- W2115619308 cites W2004927761 @default.
- W2115619308 cites W2010844841 @default.
- W2115619308 cites W2015990374 @default.
- W2115619308 cites W2017506602 @default.
- W2115619308 cites W2019203626 @default.
- W2115619308 cites W2021529748 @default.
- W2115619308 cites W2030170677 @default.
- W2115619308 cites W2031600325 @default.
- W2115619308 cites W2035690299 @default.
- W2115619308 cites W2039287713 @default.
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- W2115619308 cites W2051548651 @default.
- W2115619308 cites W2053701857 @default.
- W2115619308 cites W2055443182 @default.
- W2115619308 cites W2058217770 @default.
- W2115619308 cites W2063076247 @default.
- W2115619308 cites W2064714177 @default.
- W2115619308 cites W2070149223 @default.
- W2115619308 cites W2073919599 @default.
- W2115619308 cites W2077488136 @default.
- W2115619308 cites W2080400112 @default.
- W2115619308 cites W2086258149 @default.
- W2115619308 cites W2087867196 @default.
- W2115619308 cites W2089040602 @default.
- W2115619308 cites W2096519088 @default.
- W2115619308 cites W2100837269 @default.
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- W2115619308 cites W2110079579 @default.
- W2115619308 cites W2112533119 @default.
- W2115619308 cites W2114095434 @default.
- W2115619308 cites W2120021921 @default.
- W2115619308 cites W2121873915 @default.
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- W2115619308 cites W2142144836 @default.
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- W2115619308 cites W2154037649 @default.
- W2115619308 cites W2157450856 @default.
- W2115619308 cites W2158795933 @default.
- W2115619308 cites W2163617802 @default.
- W2115619308 cites W2164676747 @default.
- W2115619308 cites W2165286872 @default.
- W2115619308 cites W2168526937 @default.
- W2115619308 cites W2170345337 @default.
- W2115619308 cites W2170856126 @default.
- W2115619308 cites W2171030070 @default.
- W2115619308 cites W2171659436 @default.
- W2115619308 cites W2318289184 @default.
- W2115619308 cites W2324473093 @default.
- W2115619308 cites W2340572612 @default.
- W2115619308 cites W3139608143 @default.
- W2115619308 cites W4231429474 @default.
- W2115619308 cites W4238221516 @default.
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- W2115619308 doi "https://doi.org/10.1210/jc.2004-2194" @default.
- W2115619308 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1350765" @default.
- W2115619308 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15741249" @default.
- W2115619308 hasPublicationYear "2005" @default.
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