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• W2115728811 abstract "Overexpression of membrane proteins in Escherichia coli frequently leads to the formation of aggregates or inclusion bodies, which is undesirable for most studies. Ideally, one would like to optimize the expression conditions by monitoring simultaneously and rapidly both the amounts of properly folded and aggregated membrane protein, a requirement not met by any of the currently available methods. Here, we describe a simple gel-based approach with green fluorescent protein as folding indicator to detect well folded and aggregated proteins simultaneously. The method allows for rapid screening and, importantly, pinpointing the most likely bottlenecks in protein production." @default.
• W2115728811 created "2016-06-24" @default.
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• W2115728811 date "2008-04-15" @default.
• W2115728811 modified "2023-10-03" @default.
• W2115728811 title "Quality control of overexpressed membrane proteins" @default.
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• W2115728811 doi "https://doi.org/10.1073/pnas.0802190105" @default.
• W2115728811 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2311375" @default.
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