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• W2115759544 abstract "There have been a number of major advances in molecular biology in the past few years and the aim of this review is to describe some of these advances, focusing on their benefits and limitations when applied to investigating pulmonary disorders. It is written with the practising pulmonary researcher in mind, not as an introduction for the uninitiated. Useful web addresses and a list of references are included to enable interested readers to examine each technique in detail.New technologies either improve existing techniques or develop new approaches to old questions in order to generate information more quickly, easily, accurately or in a more easily repeatable fashion then existing methods.1 Some of the most powerful new technologies include polymerase chain reaction (PCR) advances, “difference analysis” (that is, the discovery of different gene expression patterns between different cells), transgenic/gene knockout technology, and gene delivery to tissues/gene therapy.Since its introduction in the 1980s PCR has become a standard tool in biomedical research. The equipment (a thermal cycler) and reagents (thermostable polymerases, oligonucleotides, etc) required for PCR are widely available and relatively inexpensive. One advantage of PCR is its extreme sensitivity which makes possible the detection and analysis of low abundance DNAs. This is especially helpful when limited amounts of starting material are available, or when few copies of the target sequence are present. Applications of PCR include the cloning of known and novel genomic DNA and cDNA sequences, DNA sequencing, construction of mutant or chimeric DNAs, and quantification of mRNA and DNA. PCR is also used in certain methods of difference analysis### PCR BASED CLONING AND SEQUENCING METHODSPCR has been used to facilitate cloning of known DNAs and to allow for the identification of novel DNAs. It can be performed directly on genomic DNA and on cDNA produced from mRNA (reverse transcription PCR (RT-PCR)). This …" @default.
• W2115759544 created "2016-06-24" @default.
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• W2115759544 date "2000-04-01" @default.
• W2115759544 modified "2023-09-27" @default.
• W2115759544 title "Recent advances in molecular biological techniques and their relevance to pulmonary research" @default.
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• W2115759544 doi "https://doi.org/10.1136/thorax.55.4.329" @default.
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