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• W2115793035 abstract "Oil palm tissue culture is subjected to indirect embryogenesis which involves five stages including mother palm selection and preparation, establishing an aseptic culture, multiplication of suitable propagules, formation of shoots and roots, and transfer to natural environment. This study aimed to investigate the effects of NAA concentrations on further improvement of oil palm (Elaeis guineensis) embryoid cultures of three selected Malaysian Palm Oil Board (MPOB) clones (PL 213, PL 209 and PL 220) and phytosterols production. Different concentrations (0, 0.5, 1.0, 2.0 mg L-1) of NAA were added to the MS basal medium and the cultures were incubated at 27 +/- 2 degreesC and 12 hours light/day photoperiod. Measurements on fresh weight of embryoids and number of shoots were recorded every two weeks throughout 16 weeks of experiment. The results indicated that PL 213 gave the highest mean of fresh weight (55.3+/-3.4 g) and number of shoots (123+/-4) in MS medium without NAA (control) followed by clones PL 209 and PL 220. There is a significant difference of clone and NAA concentration on the fresh weight of embryoid cultures but there is no significant effect of NAA concentration on the number of shoots. However, high concentration of NAA up to 2.0 mg L-1 had stimulated tissue necrosis and excretion of phenolic compounds into the culture medium. In the phytochemical study, clone PL 213 also produced the highest phytosterols in type and percentage. The useful sterol compounds that present in the extracts of PL 213 embryoid were gamma-sitosterols, beta-sitosterols, stigmasterol, campesterol, stigmasterol, 22,23-dihydro- and stigmasta-7, 22-dien-3-ol, (3.beta.,5.alpha.,22Z)-. It has been observed that higher concentration (1.0-2.0 mg L) of NAA gave poorer effects on the growth and the phytosterols production in all oil palm clones compared to control and 0.5 mg L-1 NAA used in this study." @default.
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• W2115793035 date "2014-01-01" @default.
• W2115793035 modified "2023-09-23" @default.
• W2115793035 title "The effects of different concentrations of NAA on oil palm (Elaeis guineensis) embryoid cultures and phytosterols production." @default.
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