Matches in SemOpenAlex for { <https://semopenalex.org/work/W2115802085> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W2115802085 endingPage "6118" @default.
- W2115802085 startingPage "6115" @default.
- W2115802085 abstract "The major cysteine protease of Trypanosoma cruzi, cruzain, has been previously expressed in Escherichia coli as a fusion polypeptide. The proteolytic processing events required to obtain active, mature cruzain from the recombinant expression system have been characterized using mutational analysis of the cloned gene. An inactive variant of cruzain (cruzain-C25A) revealed that the proteolytic cleavage of the COOH-terminal domain from the recombinant cruzain is independent of cruzain activity. This cleavage event, presumably performed by another protease, was reduced, although not completely eliminated, in a variant in which the cleavage recognition site was altered (cruzain-E219P). To obtain a homogeneous COOH terminus of the recombinant enzyme, a truncated form of cruzain (cruzain-delta c) was engineered by insertion of a stop codon in the gene at a site corresponding to autoproteolysis observed with the native enzyme, purified from epimastigotes. Diffraction quality crystals of recombinant cruzain (cruzain) and the truncated variant (cruzain-delta c) have been produced and characterized. Cruzain and cruzain-delta c were cocrystallized with the peptide fluoromethyl ketone (FMK) inhibitors, Z-Phe-Arg-FMK and Z-Phe-Ala-FMK, respectively, (where Z is benzyloxycarbonyl). The crystals are monoclinic, space group P2(1), with a = 45.5 A, b = 51.0 A, c = 45.7 A, and beta = 116.1 degrees. One cruzain molecule is present in the asymmetric unit. The crystallographic data reveal that the high resolution structure determination is feasible. This system will facilitate the three-dimensional structure determinations and biochemical analyses of cruzain and cruzain variants." @default.
- W2115802085 created "2016-06-24" @default.
- W2115802085 creator A5004786122 @default.
- W2115802085 creator A5050856602 @default.
- W2115802085 creator A5065094989 @default.
- W2115802085 creator A5075131235 @default.
- W2115802085 creator A5087001996 @default.
- W2115802085 date "1993-03-01" @default.
- W2115802085 modified "2023-10-03" @default.
- W2115802085 title "Production of crystallizable cruzain, the major cysteine protease from Trypanosoma cruzi." @default.
- W2115802085 cites W1541120458 @default.
- W2115802085 cites W1545290047 @default.
- W2115802085 cites W1549352256 @default.
- W2115802085 cites W1604181359 @default.
- W2115802085 cites W2005287646 @default.
- W2115802085 cites W2019981311 @default.
- W2115802085 cites W2045786862 @default.
- W2115802085 cites W2049770813 @default.
- W2115802085 cites W2050675641 @default.
- W2115802085 cites W2063459461 @default.
- W2115802085 cites W2063907318 @default.
- W2115802085 cites W2088919486 @default.
- W2115802085 cites W2180890615 @default.
- W2115802085 cites W4242257013 @default.
- W2115802085 doi "https://doi.org/10.1016/s0021-9258(18)53226-9" @default.
- W2115802085 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8454586" @default.
- W2115802085 hasPublicationYear "1993" @default.
- W2115802085 type Work @default.
- W2115802085 sameAs 2115802085 @default.
- W2115802085 citedByCount "91" @default.
- W2115802085 countsByYear W21158020852012 @default.
- W2115802085 countsByYear W21158020852013 @default.
- W2115802085 countsByYear W21158020852014 @default.
- W2115802085 countsByYear W21158020852016 @default.
- W2115802085 countsByYear W21158020852018 @default.
- W2115802085 countsByYear W21158020852019 @default.
- W2115802085 countsByYear W21158020852020 @default.
- W2115802085 countsByYear W21158020852021 @default.
- W2115802085 countsByYear W21158020852022 @default.
- W2115802085 countsByYear W21158020852023 @default.
- W2115802085 crossrefType "journal-article" @default.
- W2115802085 hasAuthorship W2115802085A5004786122 @default.
- W2115802085 hasAuthorship W2115802085A5050856602 @default.
- W2115802085 hasAuthorship W2115802085A5065094989 @default.
- W2115802085 hasAuthorship W2115802085A5075131235 @default.
- W2115802085 hasAuthorship W2115802085A5087001996 @default.
- W2115802085 hasBestOaLocation W21158020851 @default.
- W2115802085 hasConcept C112418997 @default.
- W2115802085 hasConcept C136764020 @default.
- W2115802085 hasConcept C159047783 @default.
- W2115802085 hasConcept C181199279 @default.
- W2115802085 hasConcept C185592680 @default.
- W2115802085 hasConcept C2776714187 @default.
- W2115802085 hasConcept C2777283782 @default.
- W2115802085 hasConcept C2777625466 @default.
- W2115802085 hasConcept C2779201268 @default.
- W2115802085 hasConcept C2779549974 @default.
- W2115802085 hasConcept C41008148 @default.
- W2115802085 hasConcept C55493867 @default.
- W2115802085 hasConcept C71928629 @default.
- W2115802085 hasConcept C86803240 @default.
- W2115802085 hasConcept C89423630 @default.
- W2115802085 hasConceptScore W2115802085C112418997 @default.
- W2115802085 hasConceptScore W2115802085C136764020 @default.
- W2115802085 hasConceptScore W2115802085C159047783 @default.
- W2115802085 hasConceptScore W2115802085C181199279 @default.
- W2115802085 hasConceptScore W2115802085C185592680 @default.
- W2115802085 hasConceptScore W2115802085C2776714187 @default.
- W2115802085 hasConceptScore W2115802085C2777283782 @default.
- W2115802085 hasConceptScore W2115802085C2777625466 @default.
- W2115802085 hasConceptScore W2115802085C2779201268 @default.
- W2115802085 hasConceptScore W2115802085C2779549974 @default.
- W2115802085 hasConceptScore W2115802085C41008148 @default.
- W2115802085 hasConceptScore W2115802085C55493867 @default.
- W2115802085 hasConceptScore W2115802085C71928629 @default.
- W2115802085 hasConceptScore W2115802085C86803240 @default.
- W2115802085 hasConceptScore W2115802085C89423630 @default.
- W2115802085 hasIssue "9" @default.
- W2115802085 hasLocation W21158020851 @default.
- W2115802085 hasOpenAccess W2115802085 @default.
- W2115802085 hasPrimaryLocation W21158020851 @default.
- W2115802085 hasRelatedWork W1549352256 @default.
- W2115802085 hasRelatedWork W1568483492 @default.
- W2115802085 hasRelatedWork W1999375537 @default.
- W2115802085 hasRelatedWork W2021556766 @default.
- W2115802085 hasRelatedWork W2092263395 @default.
- W2115802085 hasRelatedWork W2092697567 @default.
- W2115802085 hasRelatedWork W2103068685 @default.
- W2115802085 hasRelatedWork W2115802085 @default.
- W2115802085 hasRelatedWork W2141063039 @default.
- W2115802085 hasRelatedWork W2150558096 @default.
- W2115802085 hasVolume "268" @default.
- W2115802085 isParatext "false" @default.
- W2115802085 isRetracted "false" @default.
- W2115802085 magId "2115802085" @default.
- W2115802085 workType "article" @default.