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- W2115811082 abstract "Background: An anti-Jka was not detectable by the DiaMed-ID Micro Typing System but was strongly reactive when tested using other indirect antiglobulin test (IAT) methods. However, it was detectable in the DiaMed-ID system when the recommended diluent ID-Diluent 2, was replaced by other low ionic Strength saline (LISS) solutions. This led us to believe that the problem lay with the ID-Diluent 2. Methods: The anti-Jka was investigated using various low ionic strength diluents in the IAT against both polyspecific and monospecific antiglobulin reagents. We tested the ID-Diluent 2 for its ability to inhibit complement activation, and bind calcium. Results: The anti-Jka was shown to be a ‘complement-only’ binding antibody that reacted in all IATs performed except when tested with ID-Diluent 2. The ID-Diluent was shown to prevent haemolysis of ABO lytic antibodies, and further testing shows that it binds calcium. Conclusion: The use of a diluent which prevents haemolysis and binds calcium in an IAT system that contains anti-complement as well as anti-IgG must be questioned." @default.
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- W2115811082 date "1998-01-01" @default.
- W2115811082 modified "2023-10-17" @default.
- W2115811082 title "Complement-Binding Anti-Jka Not Detectable by DiaMed Gels" @default.
- W2115811082 cites W2022811119 @default.
- W2115811082 cites W2044983948 @default.
- W2115811082 doi "https://doi.org/10.1046/j.1423-0410.1998.7410053.x" @default.
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