FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2116014345> ?p ?o ?g. }

• W2116014345 abstract "The conventional expansion of human mesenchymal stromal cells (hMSC) for tissue engineering or (pre-) clinical investigation includes the use of 10% fetal bovine serum (FBS). However, there exists immense lot-to-lot variability in FBS samples and time consuming as well as cost intensive lot pre-testing is essential to guarantee optimal hMSC proliferation and stem cells characteristics maintenance. Furthermore, lot-to-lot variability impedes the long-term consistency of research and comparability between research groups. Therefore, we investigated the use of defined, invariable, non-synthetic FBS in low serum culture conditions for isolation and expansion of hMSC.hMSC were isolated from bone marrow in Panserin 401 supplemented with growth factors and 2% MSC-tested or non-tested, defined, invariable, non-synthetic FBS and further cultivated in vitro. The surface marker expression, differentiation capacity as well as cell proliferation and cytotoxicity was analyzed and compared between serum samples.Cells isolated and cultivated with low concentrations of MSC-tested or non-tested FBS demonstrated no differences in surface marker expression or differentiation capacity. Proliferation of hMSC was equal in medium supplemented with either serum with no indication of cell death.The low serum concentration in Panserin 401 supplemented with growth factors enables the use of defined, invariable, non-synthetic FBS for the isolation and expansion of hMSC. The required hMSC characteristics like surface marker expression and differentiation capacity are maintained. Importantly, no differences in the cell proliferation could be detected. Therefore, using these low-serum culture conditions, the need for lot-to-lot pre-testing of FBS usually needed for optimal hMSC expansion is abolished leading to long-term consistency and comparability of results." @default.
• W2116014345 created "2016-06-24" @default.
• W2116014345 creator A5039092207 @default.
• W2116014345 creator A5040539092 @default.
• W2116014345 creator A5048118430 @default.
• W2116014345 creator A5049989504 @default.
• W2116014345 creator A5060216106 @default.
• W2116014345 date "2013-02-20" @default.
• W2116014345 modified "2023-09-24" @default.
• W2116014345 title "Eliminating the need of serum testing using low serum culture conditions for human bone marrow-derived mesenchymal stromal cell expansion" @default.
• W2116014345 cites W1967241081 @default.
• W2116014345 cites W1971672573 @default.
• W2116014345 cites W1976800341 @default.
• W2116014345 cites W1977689261 @default.
• W2116014345 cites W1985619283 @default.
• W2116014345 cites W1989503157 @default.
• W2116014345 cites W1990428234 @default.
• W2116014345 cites W1993514403 @default.
• W2116014345 cites W1995469568 @default.
• W2116014345 cites W1999536207 @default.
• W2116014345 cites W2025371271 @default.
• W2116014345 cites W2034004034 @default.
• W2116014345 cites W2035866104 @default.
• W2116014345 cites W2038775969 @default.
• W2116014345 cites W2072918197 @default.
• W2116014345 cites W2078100150 @default.
• W2116014345 cites W2082098401 @default.
• W2116014345 cites W2091610929 @default.
• W2116014345 cites W2097946334 @default.
• W2116014345 cites W2105800760 @default.
• W2116014345 cites W2109606739 @default.
• W2116014345 cites W2128484946 @default.
• W2116014345 cites W2139657753 @default.
• W2116014345 cites W2141532655 @default.
• W2116014345 cites W2147381131 @default.
• W2116014345 cites W2150892075 @default.
• W2116014345 doi "https://doi.org/10.1186/1475-925x-12-15" @default.
• W2116014345 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3599911" @default.
• W2116014345 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23425366" @default.
• W2116014345 hasPublicationYear "2013" @default.
• W2116014345 type Work @default.
• W2116014345 sameAs 2116014345 @default.
• W2116014345 citedByCount "16" @default.
• W2116014345 countsByYear W21160143452015 @default.
• W2116014345 countsByYear W21160143452016 @default.
• W2116014345 countsByYear W21160143452017 @default.
• W2116014345 countsByYear W21160143452019 @default.
• W2116014345 countsByYear W21160143452020 @default.
• W2116014345 countsByYear W21160143452021 @default.
• W2116014345 countsByYear W21160143452022 @default.
• W2116014345 countsByYear W21160143452023 @default.
• W2116014345 crossrefType "journal-article" @default.
• W2116014345 hasAuthorship W2116014345A5039092207 @default.
• W2116014345 hasAuthorship W2116014345A5040539092 @default.
• W2116014345 hasAuthorship W2116014345A5048118430 @default.
• W2116014345 hasAuthorship W2116014345A5049989504 @default.
• W2116014345 hasAuthorship W2116014345A5060216106 @default.
• W2116014345 hasBestOaLocation W21160143451 @default.
• W2116014345 hasConcept C136229726 @default.
• W2116014345 hasConcept C1491633281 @default.
• W2116014345 hasConcept C16685009 @default.
• W2116014345 hasConcept C16930146 @default.
• W2116014345 hasConcept C185592680 @default.
• W2116014345 hasConcept C198826908 @default.
• W2116014345 hasConcept C203014093 @default.
• W2116014345 hasConcept C2779404806 @default.
• W2116014345 hasConcept C2780007613 @default.
• W2116014345 hasConcept C28328180 @default.
• W2116014345 hasConcept C49892992 @default.
• W2116014345 hasConcept C502942594 @default.
• W2116014345 hasConcept C54355233 @default.
• W2116014345 hasConcept C55493867 @default.
• W2116014345 hasConcept C62112901 @default.
• W2116014345 hasConcept C71924100 @default.
• W2116014345 hasConcept C81885089 @default.
• W2116014345 hasConcept C86803240 @default.
• W2116014345 hasConcept C95444343 @default.
• W2116014345 hasConceptScore W2116014345C136229726 @default.
• W2116014345 hasConceptScore W2116014345C1491633281 @default.
• W2116014345 hasConceptScore W2116014345C16685009 @default.
• W2116014345 hasConceptScore W2116014345C16930146 @default.
• W2116014345 hasConceptScore W2116014345C185592680 @default.
• W2116014345 hasConceptScore W2116014345C198826908 @default.
• W2116014345 hasConceptScore W2116014345C203014093 @default.
• W2116014345 hasConceptScore W2116014345C2779404806 @default.
• W2116014345 hasConceptScore W2116014345C2780007613 @default.
• W2116014345 hasConceptScore W2116014345C28328180 @default.
• W2116014345 hasConceptScore W2116014345C49892992 @default.
• W2116014345 hasConceptScore W2116014345C502942594 @default.
• W2116014345 hasConceptScore W2116014345C54355233 @default.
• W2116014345 hasConceptScore W2116014345C55493867 @default.
• W2116014345 hasConceptScore W2116014345C62112901 @default.
• W2116014345 hasConceptScore W2116014345C71924100 @default.
• W2116014345 hasConceptScore W2116014345C81885089 @default.
• W2116014345 hasConceptScore W2116014345C86803240 @default.
• W2116014345 hasConceptScore W2116014345C95444343 @default.
• W2116014345 hasIssue "1" @default.
• W2116014345 hasLocation W21160143451 @default.
• W2116014345 hasLocation W21160143452 @default.
• W2116014345 hasLocation W21160143453 @default.

• next