FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2116023190> ?p ?o ?g. }

• W2116023190 abstract "In Present work, the main objective is to develop less time consuming protocol for genomic DNA isolation from leaves of Passiflora foetida. Optimized protocol is cost effective, as it avoided use of expensive liquid nitrogen. The important parameters of CTAB buffer composition such as Polyvinylpyrrolidone PVP40000 (without PVP, 1%, 2%, 3.5%, 4.0%, 4.5%, 5.0%), CTAB (w, 1%, 2%, 3%, 4%, 5%), water bath temperature (30°C to 70°C) and duration on water bath for half hr and one and half hr has been optimized. CTAB (2%), PVP (1%), water bath temperature (70%), duration on water bath (1 hr) has efficiently yielded DNA quality of 200-1782 μg/0.5gm from leaf, stem, root, tendril and flower. However, 168 μg - 1782 μg of DNA has been obtained from 0.5 g of leaf of Passiflora foetida. Polyphenol contamination has been overcome using 5M NaCl and PVP. Acetate has been used for obtaining double-stranded DNA in stabilized form. Current DNA extraction protocol takes maximum of four hours for completion, which is many time savings. RAPD-PCR reaction parameters such as DNA concentration (100ng), Primer concentration (2 μM), Dream Taq polymerase (2 U), annealing temperature (29°C) and number of cycles for amplification of DNA has been optimized. Primer fragment Akansha 7 shows high polymorphism of 7 fragments ranges from 200bp - 2500 bp. Current optimized protocol of DNA isolation is specifically for Passiflora foetida, which can be used for downstream molecular techniques." @default.
• W2116023190 created "2016-06-24" @default.
• W2116023190 creator A5010429031 @default.
• W2116023190 creator A5059051289 @default.
• W2116023190 creator A5074068791 @default.
• W2116023190 date "2014-08-23" @default.
• W2116023190 modified "2023-09-27" @default.
• W2116023190 title "Efficient genomic DNA extraction protocol from medicinal rich Passiflora foetida containing high level of polysaccharide and polyphenol" @default.
• W2116023190 cites W1541180063 @default.
• W2116023190 cites W1558897642 @default.
• W2116023190 cites W1610907703 @default.
• W2116023190 cites W178751556 @default.
• W2116023190 cites W1988382377 @default.
• W2116023190 cites W1993320561 @default.
• W2116023190 cites W2006807779 @default.
• W2116023190 cites W2051955794 @default.
• W2116023190 cites W2060158198 @default.
• W2116023190 cites W2062854622 @default.
• W2116023190 cites W2079232516 @default.
• W2116023190 cites W2082091267 @default.
• W2116023190 cites W2108399382 @default.
• W2116023190 cites W2127143720 @default.
• W2116023190 cites W2131107966 @default.
• W2116023190 cites W2166384468 @default.
• W2116023190 cites W2171114940 @default.
• W2116023190 cites W2171685225 @default.
• W2116023190 cites W4240413202 @default.
• W2116023190 doi "https://doi.org/10.1186/2193-1801-3-457" @default.
• W2116023190 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4153872" @default.
• W2116023190 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25191636" @default.
• W2116023190 hasPublicationYear "2014" @default.
• W2116023190 type Work @default.
• W2116023190 sameAs 2116023190 @default.
• W2116023190 citedByCount "21" @default.
• W2116023190 countsByYear W21160231902015 @default.
• W2116023190 countsByYear W21160231902017 @default.
• W2116023190 countsByYear W21160231902018 @default.
• W2116023190 countsByYear W21160231902019 @default.
• W2116023190 countsByYear W21160231902020 @default.
• W2116023190 countsByYear W21160231902021 @default.
• W2116023190 countsByYear W21160231902022 @default.
• W2116023190 countsByYear W21160231902023 @default.
• W2116023190 crossrefType "journal-article" @default.
• W2116023190 hasAuthorship W2116023190A5010429031 @default.
• W2116023190 hasAuthorship W2116023190A5059051289 @default.
• W2116023190 hasAuthorship W2116023190A5074068791 @default.
• W2116023190 hasBestOaLocation W21160231901 @default.
• W2116023190 hasConcept C104317684 @default.
• W2116023190 hasConcept C17757408 @default.
• W2116023190 hasConcept C178790620 @default.
• W2116023190 hasConcept C185592680 @default.
• W2116023190 hasConcept C2777563447 @default.
• W2116023190 hasConcept C2778004101 @default.
• W2116023190 hasConcept C2779510327 @default.
• W2116023190 hasConcept C31903555 @default.
• W2116023190 hasConcept C43617362 @default.
• W2116023190 hasConcept C49105822 @default.
• W2116023190 hasConcept C552990157 @default.
• W2116023190 hasConcept C55493867 @default.
• W2116023190 hasConcept C59822182 @default.
• W2116023190 hasConcept C70899900 @default.
• W2116023190 hasConcept C78063203 @default.
• W2116023190 hasConcept C86803240 @default.
• W2116023190 hasConceptScore W2116023190C104317684 @default.
• W2116023190 hasConceptScore W2116023190C17757408 @default.
• W2116023190 hasConceptScore W2116023190C178790620 @default.
• W2116023190 hasConceptScore W2116023190C185592680 @default.
• W2116023190 hasConceptScore W2116023190C2777563447 @default.
• W2116023190 hasConceptScore W2116023190C2778004101 @default.
• W2116023190 hasConceptScore W2116023190C2779510327 @default.
• W2116023190 hasConceptScore W2116023190C31903555 @default.
• W2116023190 hasConceptScore W2116023190C43617362 @default.
• W2116023190 hasConceptScore W2116023190C49105822 @default.
• W2116023190 hasConceptScore W2116023190C552990157 @default.
• W2116023190 hasConceptScore W2116023190C55493867 @default.
• W2116023190 hasConceptScore W2116023190C59822182 @default.
• W2116023190 hasConceptScore W2116023190C70899900 @default.
• W2116023190 hasConceptScore W2116023190C78063203 @default.
• W2116023190 hasConceptScore W2116023190C86803240 @default.
• W2116023190 hasIssue "1" @default.
• W2116023190 hasLocation W21160231901 @default.
• W2116023190 hasLocation W21160231902 @default.
• W2116023190 hasLocation W21160231903 @default.
• W2116023190 hasLocation W21160231904 @default.
• W2116023190 hasOpenAccess W2116023190 @default.
• W2116023190 hasPrimaryLocation W21160231901 @default.
• W2116023190 hasRelatedWork W1983772289 @default.
• W2116023190 hasRelatedWork W2064266905 @default.
• W2116023190 hasRelatedWork W2143351325 @default.
• W2116023190 hasRelatedWork W2146834312 @default.
• W2116023190 hasRelatedWork W2185219645 @default.
• W2116023190 hasRelatedWork W2363829343 @default.
• W2116023190 hasRelatedWork W2370995945 @default.
• W2116023190 hasRelatedWork W2382592496 @default.
• W2116023190 hasRelatedWork W4327807839 @default.
• W2116023190 hasRelatedWork W2182825373 @default.
• W2116023190 hasVolume "3" @default.
• W2116023190 isParatext "false" @default.
• W2116023190 isRetracted "false" @default.
• W2116023190 magId "2116023190" @default.

• next