FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2116048028> ?p ?o ?g. }

• W2116048028 endingPage "16689" @default.
• W2116048028 startingPage "16683" @default.
• W2116048028 abstract "The transcription factor Oct-3/4 may be important in maintaining embryonic cells in an undifferentiated state. It is probably down-regulated at about the time that human chorionic gonadotropin (hCG) is first expressed in embryonic trophectoderm. Here we report that Oct-3/4 strongly inhibits the hCGβ subunit (hCGβ) promoter in JAr choriocarcinoma cells. Oct-3/4 reduced chloramphenicol acetyltransferase (CAT) reporter expression from the −305hCGβ promoter by about 90% in transient co-transfection assays, but had no effect on expression from the −249hCGβ promoter. The −305/−249 hCGβ fragment specifically bound synthetic Oct-3/4 protein as measured in electrophoretic mobility shift assays, and the Oct-3/4-binding site was localized around −270 by methylation interference footprinting. Site-directed mutagenesis of this binding site abolished Oct-3/4 repression. When stably transfected into JAr cells, Oct-3/4 reduced the amounts of both endogenous hCGβ messenger RNA and hCG protein to less than 10% of controls. We suggest that silencing of Oct-3/4 in trophectoderm is a prerequisite for hCG up-regulation in early human embryos at the time of maternal recognition of pregnancy. The transcription factor Oct-3/4 may be important in maintaining embryonic cells in an undifferentiated state. It is probably down-regulated at about the time that human chorionic gonadotropin (hCG) is first expressed in embryonic trophectoderm. Here we report that Oct-3/4 strongly inhibits the hCGβ subunit (hCGβ) promoter in JAr choriocarcinoma cells. Oct-3/4 reduced chloramphenicol acetyltransferase (CAT) reporter expression from the −305hCGβ promoter by about 90% in transient co-transfection assays, but had no effect on expression from the −249hCGβ promoter. The −305/−249 hCGβ fragment specifically bound synthetic Oct-3/4 protein as measured in electrophoretic mobility shift assays, and the Oct-3/4-binding site was localized around −270 by methylation interference footprinting. Site-directed mutagenesis of this binding site abolished Oct-3/4 repression. When stably transfected into JAr cells, Oct-3/4 reduced the amounts of both endogenous hCGβ messenger RNA and hCG protein to less than 10% of controls. We suggest that silencing of Oct-3/4 in trophectoderm is a prerequisite for hCG up-regulation in early human embryos at the time of maternal recognition of pregnancy." @default.
• W2116048028 created "2016-06-24" @default.
• W2116048028 creator A5024963760 @default.
• W2116048028 creator A5044756293 @default.
• W2116048028 date "1996-07-01" @default.
• W2116048028 modified "2023-09-28" @default.
• W2116048028 title "Silencing of the Gene for the β Subunit of Human Chorionic Gonadotropin by the Embryonic Transcription Factor Oct-3/4" @default.
• W2116048028 cites W1037454573 @default.
• W2116048028 cites W117387909 @default.
• W2116048028 cites W1510604115 @default.
• W2116048028 cites W1550774918 @default.
• W2116048028 cites W1557143803 @default.
• W2116048028 cites W1573499703 @default.
• W2116048028 cites W1584201864 @default.
• W2116048028 cites W1833742808 @default.
• W2116048028 cites W1932076246 @default.
• W2116048028 cites W1951882906 @default.
• W2116048028 cites W1965467790 @default.
• W2116048028 cites W1966284117 @default.
• W2116048028 cites W1971180826 @default.
• W2116048028 cites W1979981202 @default.
• W2116048028 cites W1984751884 @default.
• W2116048028 cites W2000148292 @default.
• W2116048028 cites W2004211510 @default.
• W2116048028 cites W2006499356 @default.
• W2116048028 cites W2010677182 @default.
• W2116048028 cites W2013802430 @default.
• W2116048028 cites W2017111977 @default.
• W2116048028 cites W2023116564 @default.
• W2116048028 cites W2024964870 @default.
• W2116048028 cites W2031555422 @default.
• W2116048028 cites W2036453322 @default.
• W2116048028 cites W2044794795 @default.
• W2116048028 cites W205188615 @default.
• W2116048028 cites W2113711232 @default.
• W2116048028 cites W2118290853 @default.
• W2116048028 cites W2124072977 @default.
• W2116048028 cites W2127199932 @default.
• W2116048028 cites W2149243417 @default.
• W2116048028 cites W2160826909 @default.
• W2116048028 cites W2258648827 @default.
• W2116048028 cites W2269901536 @default.
• W2116048028 cites W2278343092 @default.
• W2116048028 cites W233481338 @default.
• W2116048028 cites W248787529 @default.
• W2116048028 cites W74964901 @default.
• W2116048028 doi "https://doi.org/10.1074/jbc.271.28.16683" @default.
• W2116048028 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8663260" @default.
• W2116048028 hasPublicationYear "1996" @default.
• W2116048028 type Work @default.
• W2116048028 sameAs 2116048028 @default.
• W2116048028 citedByCount "91" @default.
• W2116048028 countsByYear W21160480282012 @default.
• W2116048028 countsByYear W21160480282013 @default.
• W2116048028 countsByYear W21160480282014 @default.
• W2116048028 countsByYear W21160480282015 @default.
• W2116048028 countsByYear W21160480282017 @default.
• W2116048028 countsByYear W21160480282019 @default.
• W2116048028 countsByYear W21160480282021 @default.
• W2116048028 crossrefType "journal-article" @default.
• W2116048028 hasAuthorship W2116048028A5024963760 @default.
• W2116048028 hasAuthorship W2116048028A5044756293 @default.
• W2116048028 hasBestOaLocation W21160480281 @default.
• W2116048028 hasConcept C104317684 @default.
• W2116048028 hasConcept C119056186 @default.
• W2116048028 hasConcept C134018914 @default.
• W2116048028 hasConcept C150194340 @default.
• W2116048028 hasConcept C153911025 @default.
• W2116048028 hasConcept C161733203 @default.
• W2116048028 hasConcept C2775948956 @default.
• W2116048028 hasConcept C54009773 @default.
• W2116048028 hasConcept C54355233 @default.
• W2116048028 hasConcept C71315377 @default.
• W2116048028 hasConcept C86803240 @default.
• W2116048028 hasConceptScore W2116048028C104317684 @default.
• W2116048028 hasConceptScore W2116048028C119056186 @default.
• W2116048028 hasConceptScore W2116048028C134018914 @default.
• W2116048028 hasConceptScore W2116048028C150194340 @default.
• W2116048028 hasConceptScore W2116048028C153911025 @default.
• W2116048028 hasConceptScore W2116048028C161733203 @default.
• W2116048028 hasConceptScore W2116048028C2775948956 @default.
• W2116048028 hasConceptScore W2116048028C54009773 @default.
• W2116048028 hasConceptScore W2116048028C54355233 @default.
• W2116048028 hasConceptScore W2116048028C71315377 @default.
• W2116048028 hasConceptScore W2116048028C86803240 @default.
• W2116048028 hasIssue "28" @default.
• W2116048028 hasLocation W21160480281 @default.
• W2116048028 hasOpenAccess W2116048028 @default.
• W2116048028 hasPrimaryLocation W21160480281 @default.
• W2116048028 hasRelatedWork W1983797650 @default.
• W2116048028 hasRelatedWork W2024974291 @default.
• W2116048028 hasRelatedWork W2082926700 @default.
• W2116048028 hasRelatedWork W2091058548 @default.
• W2116048028 hasRelatedWork W2350686596 @default.
• W2116048028 hasRelatedWork W2356576364 @default.
• W2116048028 hasRelatedWork W2387605231 @default.
• W2116048028 hasRelatedWork W2393900315 @default.
• W2116048028 hasRelatedWork W2413743288 @default.

• next