Matches in SemOpenAlex for { <https://semopenalex.org/work/W2116075556> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2116075556 endingPage "786" @default.
- W2116075556 startingPage "782" @default.
- W2116075556 abstract "N.BstNBI is a nicking endonuclease that recognizes the sequence GAGTC and nicks one DNA strand specifically. The Type IIs endonuclease, MlyI, also recognizes GAGTC, but cleaves both DNA strands. Sequence comparisons revealed significant similarities between N.BstNBI and MlyI. Previous studies showed that MlyI dimerizes in the presence of a cognate DNA, whereas N.BstNBI remains a monomer. This suggests that dimerization may be required for double-stranded cleavage. To test this hypothesis, we used a multiple alignment to design mutations to disrupt the dimerization function of MlyI. When Tyr491 and Lys494 were both changed to alanine, the mutated endonuclease, N.MlyI, no longer formed a dimer and cleaved only one DNA strand specifically. Thus, we have shown that changing the oligomerization state of an enzyme changes its enzymatic function. This experiment also established a protocol that could be applied to other Type IIs endonucleases in order to generate more novel nicking endonucleases." @default.
- W2116075556 created "2016-06-24" @default.
- W2116075556 creator A5029621251 @default.
- W2116075556 creator A5074369192 @default.
- W2116075556 date "2001-09-01" @default.
- W2116075556 modified "2023-10-17" @default.
- W2116075556 title "Converting <i>Mly</i>I endonuclease into a nicking enzyme by changing its oligomerization state" @default.
- W2116075556 cites W11401856 @default.
- W2116075556 cites W1481680796 @default.
- W2116075556 cites W1977445167 @default.
- W2116075556 cites W1986493495 @default.
- W2116075556 cites W1991877881 @default.
- W2116075556 cites W2006417881 @default.
- W2116075556 cites W2035792726 @default.
- W2116075556 cites W2042870602 @default.
- W2116075556 cites W2042936031 @default.
- W2116075556 cites W2058598696 @default.
- W2116075556 cites W2080668718 @default.
- W2116075556 cites W2118514248 @default.
- W2116075556 cites W2125074941 @default.
- W2116075556 cites W2126237980 @default.
- W2116075556 cites W2139098136 @default.
- W2116075556 cites W2179631201 @default.
- W2116075556 cites W2475083301 @default.
- W2116075556 doi "https://doi.org/10.1093/embo-reports/kve175" @default.
- W2116075556 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1084030" @default.
- W2116075556 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11520857" @default.
- W2116075556 hasPublicationYear "2001" @default.
- W2116075556 type Work @default.
- W2116075556 sameAs 2116075556 @default.
- W2116075556 citedByCount "17" @default.
- W2116075556 countsByYear W21160755562015 @default.
- W2116075556 countsByYear W21160755562019 @default.
- W2116075556 crossrefType "journal-article" @default.
- W2116075556 hasAuthorship W2116075556A5029621251 @default.
- W2116075556 hasAuthorship W2116075556A5074369192 @default.
- W2116075556 hasBestOaLocation W21160755562 @default.
- W2116075556 hasConcept C100594029 @default.
- W2116075556 hasConcept C128319531 @default.
- W2116075556 hasConcept C151730666 @default.
- W2116075556 hasConcept C153911025 @default.
- W2116075556 hasConcept C175156509 @default.
- W2116075556 hasConcept C181199279 @default.
- W2116075556 hasConcept C2777028655 @default.
- W2116075556 hasConcept C43369102 @default.
- W2116075556 hasConcept C54355233 @default.
- W2116075556 hasConcept C552990157 @default.
- W2116075556 hasConcept C55493867 @default.
- W2116075556 hasConcept C86803240 @default.
- W2116075556 hasConceptScore W2116075556C100594029 @default.
- W2116075556 hasConceptScore W2116075556C128319531 @default.
- W2116075556 hasConceptScore W2116075556C151730666 @default.
- W2116075556 hasConceptScore W2116075556C153911025 @default.
- W2116075556 hasConceptScore W2116075556C175156509 @default.
- W2116075556 hasConceptScore W2116075556C181199279 @default.
- W2116075556 hasConceptScore W2116075556C2777028655 @default.
- W2116075556 hasConceptScore W2116075556C43369102 @default.
- W2116075556 hasConceptScore W2116075556C54355233 @default.
- W2116075556 hasConceptScore W2116075556C552990157 @default.
- W2116075556 hasConceptScore W2116075556C55493867 @default.
- W2116075556 hasConceptScore W2116075556C86803240 @default.
- W2116075556 hasIssue "9" @default.
- W2116075556 hasLocation W21160755561 @default.
- W2116075556 hasLocation W21160755562 @default.
- W2116075556 hasLocation W21160755563 @default.
- W2116075556 hasLocation W21160755564 @default.
- W2116075556 hasOpenAccess W2116075556 @default.
- W2116075556 hasPrimaryLocation W21160755561 @default.
- W2116075556 hasRelatedWork W1477238300 @default.
- W2116075556 hasRelatedWork W1990139422 @default.
- W2116075556 hasRelatedWork W2004357150 @default.
- W2116075556 hasRelatedWork W2038816407 @default.
- W2116075556 hasRelatedWork W2064613642 @default.
- W2116075556 hasRelatedWork W2088519907 @default.
- W2116075556 hasRelatedWork W2417996819 @default.
- W2116075556 hasRelatedWork W2418350746 @default.
- W2116075556 hasRelatedWork W2499266855 @default.
- W2116075556 hasRelatedWork W4230367570 @default.
- W2116075556 hasVolume "2" @default.
- W2116075556 isParatext "false" @default.
- W2116075556 isRetracted "false" @default.
- W2116075556 magId "2116075556" @default.
- W2116075556 workType "article" @default.