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• W2116087032 abstract "ABSTRACT We report a search for small RNAs (sRNAs) in the low-GC, Gram-positive human pathogen Streptococcus pneumoniae . Based on bioinformatic analyses by Livny et al. (J. Livny, A. Brencic, S. Lory, and M. K. Waldor, Nucleic Acids Res. 34: 3484-3493, 2006), we tested 40 candidates by Northern blotting and confirmed the expression of nine new and one previously reported (CcnA) sRNAs in strain D39. CcnA is one of five redundant sRNAs reported by Halfmann et al. (A. Halfmann, M. Kovacs, R. Hakenbeck, and R. Bruckner, Mol. Microbiol. 66: 110-126, 2007) that are positively controlled by the CiaR response regulator. We characterized 3 of these 14 sRNAs: Spd-sr17 (144 nucleotides [nt]; decreased in stationary phase), Spd-sr37 (80 nt; strongly expressed in all growth phases), and CcnA (93 nt; induced by competence stimulatory peptide). Spd-sr17 and CcnA likely fold into structures containing single-stranded regions between hairpin structures, whereas Spd-sr37 forms a base-paired structure. Primer extension mapping and ectopic expression in deletion/insertion mutants confirmed the independent expression of the three sRNAs. Microarray analyses indicated that insertion/deletion mutants in spd-sr37 and ccnA exerted strong cis -acting effects on the transcription of adjacent genes, indicating that these sRNA regions are also cotranscribed in operons. Deletion or overexpression of the three sRNAs did not cause changes in growth, certain stress responses, global transcription, or virulence. Constitutive ectopic expression of CcnA reversed some phenotypes of D39 Δ ciaR mutants, but attempts to link CcnA to -E to comC as a target were inconclusive in ciaR + strains. These results show that S. pneumoniae , which lacks known RNA chaperones, expresses numerous sRNAs, but three of these sRNAs do not strongly affect common phenotypes or transcription patterns." @default.
• W2116087032 created "2016-06-24" @default.
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• W2116087032 date "2010-01-01" @default.
• W2116087032 modified "2023-10-16" @default.
• W2116087032 title "Identification and Characterization of Noncoding Small RNAs in <i>Streptococcus pneumoniae</i> Serotype 2 Strain D39" @default.
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• W2116087032 doi "https://doi.org/10.1128/jb.01204-09" @default.
• W2116087032 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2798261" @default.
• W2116087032 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19854910" @default.
• W2116087032 hasPublicationYear "2010" @default.
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