FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2116198813> ?p ?o ?g. }

• W2116198813 endingPage "8" @default.
• W2116198813 startingPage "461" @default.
• W2116198813 abstract "Histamine N-methyltransferase (HNMT) catalyzes the NT-methylation of histamine. The level of HNMT activity in human red blood cells is controlled by a common genetic polymorphism. We set out to clone and express a cDNA for HNMT from human tissue as a first step toward a determination of the molecular basis for this genetic polymorphism. The cloning strategy was based on possible sequence homology between rat and human kidney HNMT. Human kidney cDNA libraries were screened with the 885-nucleotide open reading frame of rat kidney HNMT cDNA. A 1.4-kilobase cDNA clone was isolated that contained two potential translation initiation codons, both in the same reading frame. The longest open reading frame of the human kidney cDNA clone contained 876 nucleotides and encoded a protein 292 amino acids in length. The amino acid sequence of this protein was 84% identical to that of rat kidney HNMT. The human kidney cDNA clone was transcribed in vitro and translated in a rabbit reticulocyte lystate system to yield a protein with an apparent molecular mass of 33 kDa, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The human kidney cDNA was also subcloned into the eukaryotic expression vector p91023(B). Partially purified HNMT isolated from the cytosol of GOS-1 cells transfected with this expression construct had biochemical properties similar to those of human kidney HNMT. Human renal cortical HNMT, partially purified human renal cortical HNMT, and partially purified transfected COS-1 cell HNMT had Km values for histamine and S-adenosyl-L-methionine, the two cosubstrates for the enzyme reaction, of 20, 13, and 14 microM and 2.0, 3.0, and 6.2 microM, respectively. IC50 values for the HNMT inhibitor amodiaquine were 0.50, 0.48, and 0.40 microM, respectively, for enzyme from these same three sources. Northern blot analyses performed with poly(A)+ RNA from a series of human tissues including kidney demonstrated three transcripts, approximately 1.3, 3.8, and 4.0 kilobases in length. Cloning of a cDNA for HNMT may now make it possible to determine the molecular basis for the HNMT genetic polymorphism in humans." @default.
• W2116198813 created "2016-06-24" @default.
• W2116198813 creator A5038929700 @default.
• W2116198813 creator A5051448032 @default.
• W2116198813 creator A5056312367 @default.
• W2116198813 creator A5069180291 @default.
• W2116198813 creator A5073064208 @default.
• W2116198813 creator A5089570861 @default.
• W2116198813 date "1994-03-01" @default.
• W2116198813 modified "2023-09-23" @default.
• W2116198813 title "Human histamine N-methyltransferase pharmacogenetics: cloning and expression of kidney cDNA." @default.
• W2116198813 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8145732" @default.
• W2116198813 hasPublicationYear "1994" @default.
• W2116198813 type Work @default.
• W2116198813 sameAs 2116198813 @default.
• W2116198813 citedByCount "13" @default.
• W2116198813 countsByYear W21161988132013 @default.
• W2116198813 countsByYear W21161988132016 @default.
• W2116198813 countsByYear W21161988132017 @default.
• W2116198813 countsByYear W21161988132019 @default.
• W2116198813 countsByYear W21161988132023 @default.
• W2116198813 crossrefType "journal-article" @default.
• W2116198813 hasAuthorship W2116198813A5038929700 @default.
• W2116198813 hasAuthorship W2116198813A5051448032 @default.
• W2116198813 hasAuthorship W2116198813A5056312367 @default.
• W2116198813 hasAuthorship W2116198813A5069180291 @default.
• W2116198813 hasAuthorship W2116198813A5073064208 @default.
• W2116198813 hasAuthorship W2116198813A5089570861 @default.
• W2116198813 hasConcept C104317684 @default.
• W2116198813 hasConcept C153911025 @default.
• W2116198813 hasConcept C167625842 @default.
• W2116198813 hasConcept C170493617 @default.
• W2116198813 hasConcept C187507357 @default.
• W2116198813 hasConcept C187882448 @default.
• W2116198813 hasConcept C189482833 @default.
• W2116198813 hasConcept C19924922 @default.
• W2116198813 hasConcept C2776885963 @default.
• W2116198813 hasConcept C34400551 @default.
• W2116198813 hasConcept C47289529 @default.
• W2116198813 hasConcept C55493867 @default.
• W2116198813 hasConcept C86803240 @default.
• W2116198813 hasConceptScore W2116198813C104317684 @default.
• W2116198813 hasConceptScore W2116198813C153911025 @default.
• W2116198813 hasConceptScore W2116198813C167625842 @default.
• W2116198813 hasConceptScore W2116198813C170493617 @default.
• W2116198813 hasConceptScore W2116198813C187507357 @default.
• W2116198813 hasConceptScore W2116198813C187882448 @default.
• W2116198813 hasConceptScore W2116198813C189482833 @default.
• W2116198813 hasConceptScore W2116198813C19924922 @default.
• W2116198813 hasConceptScore W2116198813C2776885963 @default.
• W2116198813 hasConceptScore W2116198813C34400551 @default.
• W2116198813 hasConceptScore W2116198813C47289529 @default.
• W2116198813 hasConceptScore W2116198813C55493867 @default.
• W2116198813 hasConceptScore W2116198813C86803240 @default.
• W2116198813 hasIssue "3" @default.
• W2116198813 hasLocation W21161988131 @default.
• W2116198813 hasOpenAccess W2116198813 @default.
• W2116198813 hasPrimaryLocation W21161988131 @default.
• W2116198813 hasRelatedWork W1985886875 @default.
• W2116198813 hasRelatedWork W1990070939 @default.
• W2116198813 hasRelatedWork W1990206343 @default.
• W2116198813 hasRelatedWork W2020470909 @default.
• W2116198813 hasRelatedWork W2029661895 @default.
• W2116198813 hasRelatedWork W2065739243 @default.
• W2116198813 hasRelatedWork W2074111310 @default.
• W2116198813 hasRelatedWork W2079592196 @default.
• W2116198813 hasRelatedWork W2116198813 @default.
• W2116198813 hasRelatedWork W2352079703 @default.
• W2116198813 hasVolume "45" @default.
• W2116198813 isParatext "false" @default.
• W2116198813 isRetracted "false" @default.
• W2116198813 magId "2116198813" @default.
• W2116198813 workType "article" @default.