Matches in SemOpenAlex for { <https://semopenalex.org/work/W2116202011> ?p ?o ?g. }
- W2116202011 endingPage "G802" @default.
- W2116202011 startingPage "G795" @default.
- W2116202011 abstract "We have shown that human intestinal smooth muscle cells produce IGF-I and IGF binding protein-3 (IGFBP-3). Endogenous IGF-I acts in autocrine fashion to stimulate growth of these cells. IGFBP-3 inhibits the binding of IGF-I to its receptor and thereby inhibits IGF-I-stimulated growth. In several carcinoma cell lines and some normal cells, IGFBP-3 regulates growth independently of IGF-I. Two mechanisms for this effect have been identified: IGFBP-3 can directly activate transforming growth factor-β (TGF-β) receptors or it can undergo direct nuclear translocation. The aim of the present study was to determine whether IGFBP-3 acts independently of IGF-I and to characterize the mechanisms mediating this effect in human intestinal smooth muscle cells. The direct effects of IGFBP-3 were determined in the presence of an IGF-I receptor antagonist to eliminate its IGF-I-dependent effects. Affinity labeling of TGF-β receptors (TGF-βRI, TGF-βRII, and TGF-βRV) with 125 I-labeled TGF-β1 showed that IGFBP-3 displaced binding to TGF-βRII and TGF-βRV in a concentration-dependent fashion. IGFBP-3 stimulated TGF-βRII-dependent serine phosphorylation (activation) of both TGF-βRI and of its primary substrate, Smad2(Ser 465/467 ). IGFBP-3 also caused IGF-I-independent inhibition of basal [ 3 H]thymidine incorporation. The effects of IGFBP-3 on Smad2 phosphorylation and on smooth muscle cell proliferation were independent of TGF-β1 and were abolished by transfection of Smad2 siRNA. Immunoneutralization of IGFBP-3 increased basal [ 3 H]thymidine incorporation, implying that endogenous IGFBP-3 inhibits proliferation. We conclude that endogenous IGFBP-3 directly inhibits proliferation of human intestinal smooth muscle cells by activation of TGF-βRI and Smad2, an effect that is independent of its effect on IGF-I-stimulated growth." @default.
- W2116202011 created "2016-06-24" @default.
- W2116202011 creator A5007881152 @default.
- W2116202011 creator A5057652778 @default.
- W2116202011 creator A5088499663 @default.
- W2116202011 date "2004-10-01" @default.
- W2116202011 modified "2023-10-17" @default.
- W2116202011 title "IGFBP-3 activates TGF-β receptors and directly inhibits growth in human intestinal smooth muscle cells" @default.
- W2116202011 cites W1581525584 @default.
- W2116202011 cites W1611419331 @default.
- W2116202011 cites W1920945358 @default.
- W2116202011 cites W1966905303 @default.
- W2116202011 cites W1977236952 @default.
- W2116202011 cites W1991414708 @default.
- W2116202011 cites W1991822698 @default.
- W2116202011 cites W1993535707 @default.
- W2116202011 cites W1993851041 @default.
- W2116202011 cites W1997806989 @default.
- W2116202011 cites W2001918792 @default.
- W2116202011 cites W2007006137 @default.
- W2116202011 cites W2012454322 @default.
- W2116202011 cites W2016259832 @default.
- W2116202011 cites W2020589003 @default.
- W2116202011 cites W2024707576 @default.
- W2116202011 cites W2025026333 @default.
- W2116202011 cites W2035653659 @default.
- W2116202011 cites W2037181830 @default.
- W2116202011 cites W2042663178 @default.
- W2116202011 cites W2050595371 @default.
- W2116202011 cites W2053296976 @default.
- W2116202011 cites W2054121324 @default.
- W2116202011 cites W2056718304 @default.
- W2116202011 cites W2075444548 @default.
- W2116202011 cites W2077956417 @default.
- W2116202011 cites W2080958662 @default.
- W2116202011 cites W2080977649 @default.
- W2116202011 cites W2088874555 @default.
- W2116202011 cites W2105779371 @default.
- W2116202011 cites W2111894013 @default.
- W2116202011 cites W2115417003 @default.
- W2116202011 cites W2117368084 @default.
- W2116202011 cites W2136575312 @default.
- W2116202011 cites W2140491527 @default.
- W2116202011 cites W2145887356 @default.
- W2116202011 cites W2154568166 @default.
- W2116202011 cites W2178919960 @default.
- W2116202011 cites W2181068111 @default.
- W2116202011 cites W2282727073 @default.
- W2116202011 cites W2337533984 @default.
- W2116202011 doi "https://doi.org/10.1152/ajpgi.00009.2004" @default.
- W2116202011 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15178549" @default.
- W2116202011 hasPublicationYear "2004" @default.
- W2116202011 type Work @default.
- W2116202011 sameAs 2116202011 @default.
- W2116202011 citedByCount "45" @default.
- W2116202011 countsByYear W21162020112012 @default.
- W2116202011 countsByYear W21162020112013 @default.
- W2116202011 countsByYear W21162020112014 @default.
- W2116202011 countsByYear W21162020112015 @default.
- W2116202011 countsByYear W21162020112016 @default.
- W2116202011 countsByYear W21162020112020 @default.
- W2116202011 countsByYear W21162020112021 @default.
- W2116202011 countsByYear W21162020112022 @default.
- W2116202011 crossrefType "journal-article" @default.
- W2116202011 hasAuthorship W2116202011A5007881152 @default.
- W2116202011 hasAuthorship W2116202011A5057652778 @default.
- W2116202011 hasAuthorship W2116202011A5088499663 @default.
- W2116202011 hasConcept C118131993 @default.
- W2116202011 hasConcept C11960822 @default.
- W2116202011 hasConcept C126322002 @default.
- W2116202011 hasConcept C128240485 @default.
- W2116202011 hasConcept C134018914 @default.
- W2116202011 hasConcept C153911025 @default.
- W2116202011 hasConcept C170493617 @default.
- W2116202011 hasConcept C2775960820 @default.
- W2116202011 hasConcept C54009773 @default.
- W2116202011 hasConcept C54355233 @default.
- W2116202011 hasConcept C55493867 @default.
- W2116202011 hasConcept C62112901 @default.
- W2116202011 hasConcept C71924100 @default.
- W2116202011 hasConcept C81885089 @default.
- W2116202011 hasConcept C86803240 @default.
- W2116202011 hasConcept C95444343 @default.
- W2116202011 hasConceptScore W2116202011C118131993 @default.
- W2116202011 hasConceptScore W2116202011C11960822 @default.
- W2116202011 hasConceptScore W2116202011C126322002 @default.
- W2116202011 hasConceptScore W2116202011C128240485 @default.
- W2116202011 hasConceptScore W2116202011C134018914 @default.
- W2116202011 hasConceptScore W2116202011C153911025 @default.
- W2116202011 hasConceptScore W2116202011C170493617 @default.
- W2116202011 hasConceptScore W2116202011C2775960820 @default.
- W2116202011 hasConceptScore W2116202011C54009773 @default.
- W2116202011 hasConceptScore W2116202011C54355233 @default.
- W2116202011 hasConceptScore W2116202011C55493867 @default.
- W2116202011 hasConceptScore W2116202011C62112901 @default.
- W2116202011 hasConceptScore W2116202011C71924100 @default.
- W2116202011 hasConceptScore W2116202011C81885089 @default.
- W2116202011 hasConceptScore W2116202011C86803240 @default.