FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2116328305> ?p ?o ?g. }

• W2116328305 endingPage "281" @default.
• W2116328305 startingPage "271" @default.
• W2116328305 abstract "Impaired polymorphonuclear neutrophil leukocyte (PMN) function around parturition has been associated with increased clinical mastitis in dairy cows. Rolling and attachment of PMN to the endothelium is the first step in the recruitment process and is accomplished by interaction between L-selectin on PMN and its ligand on endothelial cells. Furthermore, tyrosine phosphorylation is involved in the initiation of many PMN functions. The objective of this work was to determine changes in expression of L-selectin and tyrosine phosphorylation in the perinatal period. Eight clinically healthy Holstein cows were used as PMN donors at d-21, -14, -7,0 (calving), +1, +2, +7, +14, +28. Evaluation of L-selectin expression was carried out on activated and resting PMN. Anti-bovine L-selectin monoclonal antibody (MAB) and flow cytometric analysis were used to measure the percentage of PMN fluorescing and receptor expression (log mean fluorescent channel, LMFC). Activated and resting PMN showed similar trends in % PMN fluorescence and LM FC. The percentage of PMN fluorescing tended to decrease at parturition, followed by a significant increase at d +14 and +28 (P < 0.02). For LMFC a decrease was observed on d +1 followed by an increase through d +28 (P < 0.01). Protein tyrosine phosphorylation of lysates prepared from PMN isolated throughout the study was detected by electrophoresis and western blotting using anti-phosphotyrosine MAB. Several protein bands were tyrosine phosphorylated. Two of these bands (42-44 kDa and 90 kDa) varied in intensity over time. The intensity of the 42-44 kDa band gradually increased from d -7, peaked at d +7 (P < 0.03), and steadily decreased to d +28 (P < 0.02). Antibody to activated mitogen protein kinase reacted with the 42-44 kDa band. Reduced PMN function during the periparturient period could be related to reduced L-selectin adhesion molecules on the cell surface, and to modulation in the phosphorylation of functionally important molecules." @default.
• W2116328305 created "2016-06-24" @default.
• W2116328305 creator A5001085423 @default.
• W2116328305 creator A5003642180 @default.
• W2116328305 creator A5015857547 @default.
• W2116328305 creator A5047700911 @default.
• W2116328305 creator A5074804993 @default.
• W2116328305 creator A5075985810 @default.
• W2116328305 creator A5079570045 @default.
• W2116328305 date "2002-05-01" @default.
• W2116328305 modified "2023-10-17" @default.
• W2116328305 title "Evaluation of L-selectin expression and assessment of protein tyrosine phosphorylation in bovine polymorphonuclear neutrophil leukocytes around parturition" @default.
• W2116328305 cites W1460931276 @default.
• W2116328305 cites W1492387747 @default.
• W2116328305 cites W1538637439 @default.
• W2116328305 cites W1554506277 @default.
• W2116328305 cites W15859992 @default.
• W2116328305 cites W173581124 @default.
• W2116328305 cites W187946142 @default.
• W2116328305 cites W1966889578 @default.
• W2116328305 cites W1985719113 @default.
• W2116328305 cites W1999409067 @default.
• W2116328305 cites W2014239967 @default.
• W2116328305 cites W2016564263 @default.
• W2116328305 cites W2017604478 @default.
• W2116328305 cites W2028558313 @default.
• W2116328305 cites W2032494769 @default.
• W2116328305 cites W2053286013 @default.
• W2116328305 cites W2054050862 @default.
• W2116328305 cites W2079096224 @default.
• W2116328305 cites W2084089317 @default.
• W2116328305 cites W2084754776 @default.
• W2116328305 cites W2093547993 @default.
• W2116328305 cites W2100401567 @default.
• W2116328305 cites W2128256270 @default.
• W2116328305 cites W2141708077 @default.
• W2116328305 cites W2144220318 @default.
• W2116328305 cites W2152505433 @default.
• W2116328305 cites W2168566305 @default.
• W2116328305 cites W2183062661 @default.
• W2116328305 cites W2338553531 @default.
• W2116328305 cites W2344515317 @default.
• W2116328305 cites W2394816906 @default.
• W2116328305 cites W2403585792 @default.
• W2116328305 cites W2405602360 @default.
• W2116328305 cites W2412217786 @default.
• W2116328305 cites W2419632519 @default.
• W2116328305 cites W2438470838 @default.
• W2116328305 cites W2473564615 @default.
• W2116328305 cites W78441357 @default.
• W2116328305 cites W2436447095 @default.
• W2116328305 doi "https://doi.org/10.1051/vetres:2002015" @default.
• W2116328305 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12056478" @default.
• W2116328305 hasPublicationYear "2002" @default.
• W2116328305 type Work @default.
• W2116328305 sameAs 2116328305 @default.
• W2116328305 citedByCount "21" @default.
• W2116328305 countsByYear W21163283052012 @default.
• W2116328305 countsByYear W21163283052013 @default.
• W2116328305 countsByYear W21163283052014 @default.
• W2116328305 countsByYear W21163283052015 @default.
• W2116328305 countsByYear W21163283052017 @default.
• W2116328305 countsByYear W21163283052018 @default.
• W2116328305 countsByYear W21163283052021 @default.
• W2116328305 crossrefType "journal-article" @default.
• W2116328305 hasAuthorship W2116328305A5001085423 @default.
• W2116328305 hasAuthorship W2116328305A5003642180 @default.
• W2116328305 hasAuthorship W2116328305A5015857547 @default.
• W2116328305 hasAuthorship W2116328305A5047700911 @default.
• W2116328305 hasAuthorship W2116328305A5074804993 @default.
• W2116328305 hasAuthorship W2116328305A5075985810 @default.
• W2116328305 hasAuthorship W2116328305A5079570045 @default.
• W2116328305 hasBestOaLocation W21163283051 @default.
• W2116328305 hasConcept C104317684 @default.
• W2116328305 hasConcept C11960822 @default.
• W2116328305 hasConcept C126322002 @default.
• W2116328305 hasConcept C134018914 @default.
• W2116328305 hasConcept C153911025 @default.
• W2116328305 hasConcept C155619193 @default.
• W2116328305 hasConcept C159654299 @default.
• W2116328305 hasConcept C16685009 @default.
• W2116328305 hasConcept C170493617 @default.
• W2116328305 hasConcept C198677920 @default.
• W2116328305 hasConcept C203014093 @default.
• W2116328305 hasConcept C2776165026 @default.
• W2116328305 hasConcept C2776914184 @default.
• W2116328305 hasConcept C2777553839 @default.
• W2116328305 hasConcept C2777983669 @default.
• W2116328305 hasConcept C2779835222 @default.
• W2116328305 hasConcept C542903549 @default.
• W2116328305 hasConcept C553184892 @default.
• W2116328305 hasConcept C55493867 @default.
• W2116328305 hasConcept C71924100 @default.
• W2116328305 hasConcept C85265743 @default.
• W2116328305 hasConcept C86803240 @default.
• W2116328305 hasConceptScore W2116328305C104317684 @default.
• W2116328305 hasConceptScore W2116328305C11960822 @default.
• W2116328305 hasConceptScore W2116328305C126322002 @default.

• next