Matches in SemOpenAlex for { <https://semopenalex.org/work/W2116344057> ?p ?o ?g. }
- W2116344057 endingPage "2219" @default.
- W2116344057 startingPage "2208" @default.
- W2116344057 abstract "Formins are conserved actin nucleators which promote the assembly of actin filaments for the formation of diverse actin structures. In fission yeast Schizosaccharomyces pombe, the formin cdc12p is required specifically in assembly of the actin-based contractile ring during cytokinesis. Here, using a mutational analysis of cdc12p, we identify regions of cdc12p responsible for ring assembly and localization. Profilin-binding residues of the FH1 domain regulate actin assembly and processive barbed-end capping by the FH2 domain. Studies using photobleaching (FRAP) and sensitivity to latrunculin A treatment show that profilin binding modulates the rapid dynamics of actin and cdc12p within the ring in vivo. Visualized by functional GFP-fusion constructs expressed from the endogenous promoter, cdc12p appears in a small number of cytoplasmic motile spot structures that deliver the formin to the ring assembly site, without detectable formation of an intermediate band of “nodes.” The FH3/DID region directs interphase spot localization, while an N-terminal region and the FH1-FH2 domains of cdc12p can target its localization to the ring. Mutations in putative DID and DAD regions do not alter regulation, suggesting that cdc12p is not regulated by a canonical autoinhibition mechanism. Our findings provide insights into the regulation of formin activity and the mechanisms of contractile ring dynamics and assembly." @default.
- W2116344057 created "2016-06-24" @default.
- W2116344057 creator A5009297379 @default.
- W2116344057 creator A5033446738 @default.
- W2116344057 creator A5062225126 @default.
- W2116344057 creator A5075839935 @default.
- W2116344057 creator A5081809572 @default.
- W2116344057 creator A5082789932 @default.
- W2116344057 date "2008-05-01" @default.
- W2116344057 modified "2023-10-15" @default.
- W2116344057 title "Regulation and Targeting of the Fission Yeast Formin cdc12p in Cytokinesis" @default.
- W2116344057 cites W1538692246 @default.
- W2116344057 cites W1585344957 @default.
- W2116344057 cites W1595495768 @default.
- W2116344057 cites W1600241919 @default.
- W2116344057 cites W1754544241 @default.
- W2116344057 cites W1950490249 @default.
- W2116344057 cites W1972250746 @default.
- W2116344057 cites W1972923493 @default.
- W2116344057 cites W1973870846 @default.
- W2116344057 cites W1980086614 @default.
- W2116344057 cites W1984063704 @default.
- W2116344057 cites W1989133939 @default.
- W2116344057 cites W1990265320 @default.
- W2116344057 cites W1990356394 @default.
- W2116344057 cites W1990850141 @default.
- W2116344057 cites W1994844322 @default.
- W2116344057 cites W1998143618 @default.
- W2116344057 cites W2008282204 @default.
- W2116344057 cites W2011343310 @default.
- W2116344057 cites W2017214363 @default.
- W2116344057 cites W2018480878 @default.
- W2116344057 cites W2019559746 @default.
- W2116344057 cites W2023434864 @default.
- W2116344057 cites W2024095351 @default.
- W2116344057 cites W2025175085 @default.
- W2116344057 cites W2027866287 @default.
- W2116344057 cites W2029042183 @default.
- W2116344057 cites W2030222262 @default.
- W2116344057 cites W2030768863 @default.
- W2116344057 cites W2032280683 @default.
- W2116344057 cites W2036535918 @default.
- W2116344057 cites W2037611941 @default.
- W2116344057 cites W2042069042 @default.
- W2116344057 cites W2047014275 @default.
- W2116344057 cites W2047853156 @default.
- W2116344057 cites W2048494335 @default.
- W2116344057 cites W2050301961 @default.
- W2116344057 cites W2056889742 @default.
- W2116344057 cites W2058377873 @default.
- W2116344057 cites W2059746552 @default.
- W2116344057 cites W2062852486 @default.
- W2116344057 cites W2063693682 @default.
- W2116344057 cites W2064071063 @default.
- W2116344057 cites W2066486192 @default.
- W2116344057 cites W2072788367 @default.
- W2116344057 cites W2077593465 @default.
- W2116344057 cites W2082677295 @default.
- W2116344057 cites W2083523423 @default.
- W2116344057 cites W2085308794 @default.
- W2116344057 cites W2090221607 @default.
- W2116344057 cites W2091060631 @default.
- W2116344057 cites W2094269474 @default.
- W2116344057 cites W2103966138 @default.
- W2116344057 cites W2106723342 @default.
- W2116344057 cites W2114580275 @default.
- W2116344057 cites W2121944671 @default.
- W2116344057 cites W2125028666 @default.
- W2116344057 cites W2127303707 @default.
- W2116344057 cites W2127735672 @default.
- W2116344057 cites W2129890878 @default.
- W2116344057 cites W2132088939 @default.
- W2116344057 cites W2136955958 @default.
- W2116344057 cites W2139582633 @default.
- W2116344057 cites W2142173778 @default.
- W2116344057 cites W2146099171 @default.
- W2116344057 cites W2147624113 @default.
- W2116344057 cites W2153290005 @default.
- W2116344057 cites W2155353685 @default.
- W2116344057 cites W2158045056 @default.
- W2116344057 cites W2160943729 @default.
- W2116344057 cites W2187601539 @default.
- W2116344057 cites W2342986259 @default.
- W2116344057 doi "https://doi.org/10.1091/mbc.e07-07-0731" @default.
- W2116344057 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2366869" @default.
- W2116344057 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18305104" @default.
- W2116344057 hasPublicationYear "2008" @default.
- W2116344057 type Work @default.
- W2116344057 sameAs 2116344057 @default.
- W2116344057 citedByCount "78" @default.
- W2116344057 countsByYear W21163440572012 @default.
- W2116344057 countsByYear W21163440572013 @default.
- W2116344057 countsByYear W21163440572014 @default.
- W2116344057 countsByYear W21163440572015 @default.
- W2116344057 countsByYear W21163440572016 @default.
- W2116344057 countsByYear W21163440572017 @default.
- W2116344057 countsByYear W21163440572018 @default.
- W2116344057 countsByYear W21163440572019 @default.